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Preparation method of sesquiterpenes saussurea lappa clarke alkaloid monomer

A technology for sesquiterpenes and alkaloids, which is applied in the field of natural product separation, can solve the problems such as no reports on the simultaneous preparation of sesquiterpenoid alkaloids in S. Ease of Industrialization

Inactive Publication Date: 2013-01-09
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the existing literature, there are relatively few reports on the research on the alkaloids in Cinnamonia, and there is no report on the method for the simultaneous preparation of sesquiterpene alkaloids in Cinnamonia

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take 50kg of fenugreek root and pulverize it into a coarse powder and put it into a microwave extraction tank, add 85% ethanol solution 10 times the weight of the raw material for microwave extraction for 20 minutes, and filter out the extract, repeat the extraction twice in this way, combine the extracts, and recover the filtrate under reduced pressure Obtain the crude extract, suspend and dissolve the crude extract in water, extract 4 times with 2 times the volume of ethyl acetate, concentrate under reduced pressure to obtain ethyl acetate extract, dissolve the ethyl acetate extract with methanol and apply The sample was sent to a 200-300-mesh medium-pressure magnesia column, and a mixed solvent of petroleum ether-acetone was used to carry out gradient elution according to the volume ratio of 20:1, 10:1, 5:1, 2:1, and 1:1. Collect the fractions in sections, combine the fractions containing the sesquiterpene alkaloids of fenugreek, recover the solvent to a small volume ...

Embodiment 2

[0021] Take 50kg of fenugreek root and pulverize it into a coarse powder and put it into a microwave extraction tank, add 70% ethanol solution 15 times the weight of the raw material for microwave extraction for 20 minutes, and filter out the extract, repeat the extraction for 3 times, combine the extracts, and recover the filtrate under reduced pressure Obtain the crude extract, suspend and dissolve the crude extract in water, extract 4 times with 1 times the volume of ethyl acetate, concentrate under reduced pressure to obtain ethyl acetate extract, dissolve the ethyl acetate extract with methanol and apply The sample was sent to a 200-300-mesh medium-pressure magnesia column, and a mixed solvent of petroleum ether-acetone was used to carry out gradient elution according to the volume ratio of 20:1, 10:1, 5:1, 2:1, and 1:1. Collect the fractions in sections, combine the fractions containing the sesquiterpene alkaloids of fenugreek, recover the solvent to a small volume under ...

Embodiment 3

[0023] Take 50kg of fenugreek root and pulverize it into a coarse powder and put it into a microwave extraction tank, add 80% ethanol solution 8 times the weight of raw materials for microwave extraction for 15 minutes, and filter out the extract, repeat the extraction for 3 times, combine the extracts, and recover the filtrate under reduced pressure Obtain the crude extract, suspend and dissolve the crude extract in water, extract 4 times with 4 times the volume of ethyl acetate, concentrate under reduced pressure to obtain ethyl acetate extract, dissolve the ethyl acetate extract with methanol and apply The sample was sent to a medium-pressure magnesium oxide column with a filler of 100-200 mesh, and the mixed solvent of petroleum ether-acetone was used for gradient elution according to the volume ratio of 20:1, 10:1, 5:1, 2:1, and 1:1. Collect the fractions in sections, combine the fractions containing the sesquiterpene alkaloids of fenugreek, recover the solvent to a small ...

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Abstract

The invention belongs to the filed of natural product separating, and provides a preparation method of sesquiterpenes saussurea lappa clarke alkaloid monomer. The preparation method comprises the steps of extracting with ethanol solution, extracting with organic solvent, carrying out middle-pressure magnesium oxide column chromatography, and purifying with preparative high performance liquid chromatography. The product prepared by the preparation method provided by the invention is high in purity, high in quality, simple in preparation method, large in preparation amount, and easy for industrialization.

Description

technical field [0001] The invention belongs to the field of separation of natural products, and in particular relates to a method for simultaneously preparing balsamic amine A and basilica amine B from balsa root by using high-performance liquid phase preparative chromatography. Background technique [0002] Asteraceae is a plant of Asteraceae Saussurea lappa The root of Clarke, also known as Muxiang and Guangmuxiang, has the effects of moving qi and dissolving stagnation, soothing the liver, and strengthening the stomach. It can treat qi stagnation, food accumulation, chest fullness, abdominal distension, vomiting and diarrhea. Contains a variety of volatile oils and alkaloid compounds in cloud wood incense. [0003] Ceramide A and Ceramide B are sesquiterpene alkaloid compounds isolated from the root of Ceruleus balm, both components have anti-ulcer effect, Ceramide A at 100mg / kg , the inhibition rate of gastric ulcer in rats induced by HCl / ethanol was 59.8%, and when ...

Claims

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Application Information

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IPC IPC(8): C07D405/06
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV
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