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Method for preparing nanofiber by electrostatic spinning of collagen aqueous solution

A collagen fiber, collagen technology, applied in the direction of single-component protein rayon, fiber processing, fiber chemical characteristics, etc., can solve the problems of growth factor inactivation, collagen degeneration, cell death, etc., to achieve stable physical and chemical properties, degeneration Low-level, non-cytotoxic effect

Inactive Publication Date: 2013-01-16
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Whether cells, cytokines or growth factors are added to the electrospinning process, or the spinning products are used as drugs, gene carriers, cell scaffolds and devices, the toxicity of solvents or residual solvents (such as various fluorinated solvents) cannot be ignored. , various methods of removing residual solvents from electrospinning products may lead to collagen denaturation, growth factor inactivation, cell death

Method used

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  • Method for preparing nanofiber by electrostatic spinning of collagen aqueous solution
  • Method for preparing nanofiber by electrostatic spinning of collagen aqueous solution
  • Method for preparing nanofiber by electrostatic spinning of collagen aqueous solution

Examples

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Effect test

Embodiment 1

[0036] The preparation method of collagen nanofiber comprises the following steps:

[0037] (1) Preparation of collagen electrospinning stock solution

[0038] Add 20mg of reconstructed collagen fibers with a molecular weight greater than or equal to 250KDa to 1g of an acidic aqueous solution whose pH value is adjusted to 2 with acetic acid, and dissolve it into a uniform solution at a temperature of 30°C using magnetic force or mechanical stirring to obtain an electrospinning stock solution ;

[0039] (2) Electrospinning of collagen nanofibers

[0040] The above electrospinning stock solution was subjected to electrospinning at an electric field strength of 360 KV / m; a spinneret hole diameter of 5 mm; a receiving distance of 9 cm; and a spinning rate of 0.2 ml / h to obtain collagen nanofibers.

Embodiment 2

[0042] The preparation method of collagen nanofiber comprises the following steps:

[0043] (1) Preparation of collagen electrospinning stock solution

[0044] Add 25mg of reconstructed collagen fibers with a molecular weight greater than or equal to 250KDa to 1g of an acidic aqueous solution whose pH value is adjusted to 2 with hydrochloric acid, and dissolve it into a uniform solution at a temperature of 20°C using magnetic force or mechanical stirring to obtain an electrospinning stock solution ;

[0045] (2) Electrospinning of collagen nanofibers

[0046]The above electrospinning stock solution was subjected to electrospinning at an electric field strength of 340 KV / m; a spinneret hole diameter of 6 mm; a receiving distance of 7 cm; and a spinning rate of 0.4 ml / h to obtain collagen nanofibers.

Embodiment 3

[0048] The preparation method of collagen nanofiber comprises the following steps:

[0049] (1) Preparation of collagen electrospinning stock solution

[0050] Add 100mg of reconstructed collagen fibers with a molecular weight greater than or equal to 250KDa to 1g of an acidic aqueous solution whose pH value is adjusted to 3 with citric acid, and dissolve it into a uniform solution at a temperature of 10°C with magnetic or mechanical stirring to obtain an electrospun fiber stock solution;

[0051] (2) Electrospinning of collagen nanofibers

[0052] The above electrospinning stock solution was electrostatically spun at an electric field strength of 280KV / m; a spinneret hole diameter of 7mm; a receiving distance of 8cm; and a spinning rate of 0.3ml / h to obtain collagen nanofibers.

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Abstract

The invention discloses a method for preparing nanofiber by electrostatic spinning of a collagen aqueous solution. The method is characterized in that 20 to 300 weight parts of reconstruction collagen fiber with molecular weight larger than or equal to 250 KDa are added in 1,000 weight parts of acid aqueous solution with the pH value regulated by acid to be 2 to 4, and are stirred and dissolved magnetically and mechanically to a uniform solution to obtain an electrostatic spinning raw solution at the temperature of 4 to 30 DEG C, and the electrostatic spinning raw solution is subjected to electrostatic spinning under the conditions that the electric field strength is 140 to 360 kV / m, the spinneret orifice diameter is 5 to 7 mm, and the spinning speed is 0.2 to 0.5 ml / h to obtain the collagen nanofiber. The preparation process of the collagen nanofiber is non-toxic and pollution-free, and the prepared collagen nanofiber has the advantages of low denaturation degree, stable physicochemical property, no cytotoxicity, biological activity and low cost.

Description

technical field [0001] The invention relates to a method for preparing nanofibers by electrostatic spinning of collagen aqueous solution, and belongs to the field of preparation of biomedical materials, tissue engineering scaffolds and drug slow-release materials. Background technique [0002] Electrospinning of collagen is one of the most interesting issues in spinning technology. Collagen is an important component of animal connective tissue, present in tissues and organs. As a natural macromolecule, it has a series of advantages such as promoting cell growth, low antigenicity, and biodegradability when used as a biological material. In the research and development of tissue engineering, drug sustained release, and medical devices, obtaining collagen nanofibers is attractive, and it is the basis of these research work or product development. Regrettably, the electrospinning of collagen has not yet found a satisfactory technical method. [0003] One of the key technologi...

Claims

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Application Information

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IPC IPC(8): D01F4/00D01F1/10D01D5/00
Inventor 蒋波万法盛李霞陈露李莉莉
Owner SICHUAN UNIV