Method for extracting and separating ginkgolide B from ginkgo leaves

A technology of ginkgolide and ginkgo biloba, applied in the direction of organic chemistry, etc., can solve the problems of ginkgolide B, such as complex process, difficult industrial production, and high cost, and achieve the effects of good dispersibility, small solvent loss, and low cost

Active Publication Date: 2014-12-03
黑龙江天宏药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The purpose of the present invention is to provide a method for extracting and separating ginkgolide B from ginkgo leaves in order to solve the problems of complex extraction process of ginkgolide B, high cost, low purity, low yield and difficulty in industrial production in the existing method

Method used

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Examples

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Effect test

specific Embodiment approach 1

[0027] Specific embodiment one: a kind of method of extracting and separating ginkgolide B from ginkgo leaf of the present embodiment is as follows:

[0028] 1. Take ginkgo biloba leaves, add 10 to 15 times the mass of ethanol solution with a mass percentage of 50% to 95%, heat and reflux for 1 to 3 hours, filter, collect the filtrate, concentrate under reduced pressure to remove ethanol, and obtain concentrated solution A;

[0029] 2. Add water to the concentrated solution A obtained in step 1 to dissolve until the concentrated solution A contains 0.1-0.3 g / mL of crude drug, then place it at room temperature for 24-48 hours, take the upper layer, and set aside;

[0030] 3. Concentrate the supernatant liquid obtained in step 2 under reduced pressure until the crude drug content is 0.5~1.5g / mL, then pass through the macroporous resin column at a speed of 0.5~3BV / h, and first elute with water at a speed of 0.5~3BV / h, Then use ethanol solution with a mass percentage of 50%~95% to...

specific Embodiment approach 2

[0039] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the ginkgo leaf described in step one is ginkgo leaf coarse powder. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0040] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the macroporous resin described in step three is one or more of NKA-9, ADS-2, SA-1, SA-2 A mixture composed in any ratio. Others are the same as in the first or second embodiment.

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Abstract

The invention discloses a method for extracting and separating ginkgolide B from ginkgo leaves. The method comprises the following steps of: extracting the ginkgo leaves with ethanol; performing reduced-pressure concentration of the extracting solution to obtain extract; adding water to dilute the extract, and adsorbing with a macroporous resin column; eluting with ethanol; performing reduced-pressure concentration on the eluent to obtain thick paste; uniformly mixing the thick paste with a diluent, and drying; packing the dry paste, eluting with ethyl acetate, and concentrating the ethyl acetate eluent until the smell of ethyl acetate does not exist; adding ethanol for dissolution; crystallizing with ethanol to obtain coarse crystal; and re-crystallizing the coarse crystal with ethanol and ethyl acetate to obtain the ginkgolide B with purity greater than or equal to 98%. According to the method provided by the invention, the technology is simple, the production cost is relatively low, the product purity is high, the yield is relatively high, and the method is favorable for industrial production.

Description

technical field [0001] The invention relates to a method for extracting ginkgolide B. Background technique [0002] Ginkgolide is currently one of the most powerful and specific platelet activating factor (PAF) receptor blockers. Ginkgolide B has the strongest blocking effect on PAF receptor among ginkgolides, which can be eliminated in a dose-dependent manner. Platelet aggregation caused by PAF, thereby preventing the formation of thrombus. Pharmacological studies have shown that ginkgolide B has anti-allergic, anti-inflammatory, anti-shock effects, and also has protective effects on ischemic injury and organ transplant rejection. Ginkgolide B is relatively low in the commercially available Ginkgo biloba extract and has a specific structure. It is difficult to obtain high-purity and high-yield Ginkgolide B through extraction and separation. [0003] The inventors have consulted domestic and foreign literature, and the authorized inventions or patents directly related to t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D493/22
Inventor 宁树一魏凯马仁强周清
Owner 黑龙江天宏药业股份有限公司
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