Flavonoids from nervilia fordii and preparation method and use thereof

A technology of flavonoids and uses, applied in the field of medicine, can solve the problems of rare antibacterial experimental research, retention, and few evaluations of antibacterial activity, and achieve good medicinal prospects

Inactive Publication Date: 2013-01-23
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although there are many components with antibacterial activity in Chinese herbal medicines, many researches at present only stay at the antibacterial research level of Chinese herbal medicine decoction, crude extract and volatile oil, or one or two monomer components. There are few in vivo and in vitro antibacterial experiments of phytochemical components. Effective monomer compounds are extracted from them to determine their chemical structures, and combined with an appropriate antibacterial activity screening system, there are not many antibacterial activity evaluations on chemical components of traditional Chinese medicine.

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  • Flavonoids from nervilia fordii and preparation method and use thereof
  • Flavonoids from nervilia fordii and preparation method and use thereof
  • Flavonoids from nervilia fordii and preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Take 5 kg of dry whole herb of Nervilia fordii (Hance) Schltr., add 10 times the amount of 60% ethanol to reflux and extract 3 times, each time for 2 hours, combine the extracts, and use a rotary evaporator at a temperature lower than 45 ° C. After recovering ethanol under reduced pressure, 600 g of extract was obtained; the extract was suspended in water to obtain an aqueous suspension of extract, and the aqueous suspension of extract was extracted three times with cyclohexane, and the cyclohexane layers were combined; Ethyl extracts the aqueous extract suspension after extracting the extract aqueous suspension with cyclohexane three times, and combines the ethyl acetate layer; then uses n-butanol to extract the extract aqueous suspension with ethyl acetate The extract water suspension was extracted three times, and the n-butanol layers were combined; the cyclohexane layer solvent cyclohexane, the ethyl acetate layer solvent ethyl acetate, and the After the butanol lay...

Embodiment 2

[0054] Take 10kg of dried Nervilia fordii (Hance) Schltr. whole herb, add 7 times the amount of 80% ethanol to reflux and extract twice, each time for 3 hours, combine the extracts, and use a rotary evaporator at a temperature lower than 45°C After recovering ethanol under reduced pressure, 1200 g of extract was obtained. Suspend the extract in water to obtain an aqueous suspension of extract, extract the aqueous suspension of extract three times with cyclohexane, combine the cyclohexane layers; then extract the aqueous extract with cyclohexane with ethyl acetate The aqueous extract suspension after the suspension was extracted three times, and the ethyl acetate layer was combined; then, the aqueous extract suspension was extracted three times with n-butanol after the aqueous extract suspension was extracted with ethyl acetate, and the normal ethyl acetate layer was combined. butanol layer; use a rotary evaporator to recover the cyclohexane layer solvent cyclohexane, the ethyl...

experiment example 1

[0059] Compound 1, yellow powder, odorless, melting point: 154°C-156°C (methanol). High-resolution ESI-MS gives molecular ion peaks: [M+H + ]m / z 697.2689, the molecule is: C 31 h 36 o 18 , Molecular weight: 696.61. IR spectrum at 3393cm -1 There is a strong broad peak, suggesting that there is a hydroxyl group in the molecule, 1655cm -1 The strong absorption peaks at 1600 and 1499cm suggest that there is a carbonyl group in the structure -1 The absorption peaks suggest that there is a benzene ring in the structure. The chemical name of compound 1 is: (3,4,5-trihydroxy-6-(7-methoxy-5-hydroxy-2-(3-methoxy-4-(3,4,5-trihydroxy -6-Hydroxymethyl-tetrahydro-2H-pyran-2-acyloxy)phenyl)-4-oxo-4H-benzopyran-3-acyloxy)-tetrahydro-2H-pyran ) methyl acetate.

[0060] of the compound 1 H-NMR (600MHz, DMSO-d 6 ), δ12.52 (1H, s) is the characteristic signal of the 5-hydroxyl proton of flavone, δ6.81 (1H, d, J = 2.0Hz), 6.40 (1H, d, J = 2.0Hz) is the 5, 7 di Oxygen-substituted flavo...

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Abstract

The invention discloses flavonoids from nervilia fordii and a preparation method and use thereof. The flavonoids are two compounds: the first is methylrhamnetin-3-O-beta-glucopyranosyl-4'-O-beta-6'''-acetyl-glucoside, namely neRvilifoRdin in chemistry; the second is methylrhamnetin-4-O-beta-glucopyranosyl-3-O-beta-6''-acetyl-glucoside, namely nervilifordin F. The preparation method, which is based on dry whole nervilia fordii as raw material, comprises the following steps of: 1) preparing an extract; 2) preparing a cyclohexane layered extract, an ethyl acetate layered extract, n-butyl alcohol layered extract and water layered extract; and 3) preparing methylrhamnetin-3-O-beta-glucopyranosyl-4'-O-beta-6'''z-acetyl-glucoside and methylrhamnetin-4'-O-beta-glucopyranosyl-3-O-beta-6''-acetyl-glucoside. The flavonoids are used for preparing antibacterial and / or anti-inflammatory medicines.

Description

technical field [0001] The invention belongs to the technical field of medicine, in particular to flavonoids in blue skyflower and its preparation method and application. Background technique [0002] At present, although there are many antibiotics and chemically synthesized antibacterial drugs for clinical use, due to the extensive application of broad-spectrum antibacterial drugs and the widespread adoption of interventional diagnosis and treatment, the problem of bacterial drug resistance is becoming more and more serious, and the adverse reactions of drugs are also increasing. In addition, the development of chemically synthesized drugs is difficult, the cycle is long, and the toxicity and side effects are obvious. Therefore, the search for highly effective, low-toxic, and sensitive antibacterial drugs from natural drugs has attracted the attention of medical workers at home and abroad. In 2003, my country's use of integrated traditional Chinese and Western medicine to p...

Claims

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Application Information

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IPC IPC(8): C07H17/07C07H1/08A61K31/7048A61P29/00A61P31/00
Inventor 邱莉谢集照姚新生缪剑华谢云峰黄桂坤
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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