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Culture medium for pseudomonas aeruginosa

A Pseudomonas aeruginosa, culture medium technology, applied in bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of poor culture conditions, not taking into account the limited components of Pseudomonas, and the small number of microorganisms, and achieve the goal of culturing Short time, high phenol tolerance, high vitality effect

Inactive Publication Date: 2013-01-23
ZHONGBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The essence of the above problems is that the number of microorganisms is small and the culture conditions are poor.
The reason is that the current research on the culture medium mainly focuses on the conventional nutrients such as carbon sources, nitrogen sources and inorganic salts required for the growth of the strains, and does not take into account the limiting components required for the growth of Pseudomonas in the culture medium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Weigh MnCl respectively 2 0.48g, MoSO 4 0.44g, FeSO 4 0.6g, purine base 1.4g, VB 2 7.36g and 1.12g of acetamide were added into 1000mL of deionized water, and fully dissolved to obtain a solution of trace elements and growth factors.

[0019] Take 7.6g of glucose, 3.3g of urea, 2.64g of dipotassium hydrogen phosphate, 0.86g of potassium dihydrogen phosphate, 1g of magnesium sulfate, 4.5g of sodium chloride, 1mL of trace element and growth factor solution, add deionized water and stir to dissolve completely, Make up to 1000 mL with deionized water, and adjust the pH range of the medium to 6.5-7.5 to obtain a medium for Pseudomonas aeruginosa.

Embodiment 2

[0021] Weigh MnCl respectively 2 0.52g, MoSO 4 0.5g, FeSO 4 0.63g, purine base 2.35g, VB 2 8.38g and 0.81g of acetamide were added into 1000mL of deionized water, and fully dissolved to obtain a solution of trace elements and growth factors.

[0022] Take 8g of glucose, 3.5g of urea, 2.78g of dipotassium hydrogen phosphate, 0.72g of potassium dihydrogen phosphate, 0.7g of magnesium sulfate, 4.1g of sodium chloride, 1mL of trace element and growth factor solution, add deionized water and stir to dissolve completely, Make up to 1000 mL with deionized water, and adjust the pH range of the medium to 6.5-7.5 to obtain a medium for Pseudomonas aeruginosa.

Embodiment 3

[0024] Weigh MnCl respectively 2 0.55g, MoSO 4 0.48g, FeSO 4 0.58g, purine base 1.95g, VB 2 6.93g and 0.99g of acetamide were added into 1000mL of deionized water, fully dissolved, and a solution of trace elements and growth factors was obtained.

[0025] Take glucose 9.3g, urea 3.8g, dipotassium hydrogen phosphate 2.84g, potassium dihydrogen phosphate 0.96g, magnesium sulfate 0.8g, sodium chloride 3.6g, trace element and growth factor solution 1mL, add deionized water and stir to dissolve completely , make up to 1000 mL with deionized water, and adjust the pH range of the medium to 6.5-7.5 to obtain a medium for Pseudomonas aeruginosa.

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Abstract

The invention relates to a culture medium for pseudomonas aeruginosa. Per 1000mL of culture medium contains 7-10g of glucose, 2-4g of urea, 2-3g of dipotassium phosphate, 0.5-2g of monopotassium phosphate, 0.5-1.5g of magnesium sulfate, 3-5g of sodium chloride, 1-3mL of microelement and growth factor solution and the balance of deionized water, wherein the microelement and growth factor solution contains 3.5-4.5mmol / L of MnCl2, 2.0-2.8mmol / L of MoSO4, 3.6-4.6mmol / L of FeSO4, 10-20mmol / L of purine base, 18-23mmol / L of VB2 and 12-20mmol / L of acetamide. By adopting the culture medium, favorable growth environment is provided for pseudomonas aeruginosa, the culture time of pseudomonas aeruginosa is reduced, the tolerance of strains is high, the adaptive capacity is high, the degradation efficiency of phenol reaches above 96%, and the degradation efficiency of chlorobenzene reaches above 93%.

Description

technical field [0001] The invention belongs to the technical field of microorganism cultivation, and relates to a microorganism culture medium, in particular to a culture medium for cultivating Pseudomonas aeruginosa. Background technique [0002] Phenol-containing wastewater is a kind of industrial wastewater with a wide range of pollution and a large amount of discharge, which is harmful to agricultural production and the growth and reproduction of animals and plants. If it is discharged without treatment, it will threaten human health. When the intake of phenol reaches a certain value, it will cause acute poisoning, specifically manifested as headache, fatigue, blurred vision, and edema. At present, there are mainly physical, chemical and biological methods for treating phenolic wastewater at home and abroad. There are many chemical and physical methods to treat phenolic wastewater, but the problem of secondary pollution is common. Biological methods, especially the us...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/385
Inventor 贾万利李颖赵文英
Owner ZHONGBEI UNIV