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Intestinal epithelial cell line of pheretima aspergillum and construction method for intestinal epithelial cell line

A technology of epithelial cells and ring hairs, applied to animal cells, etc., can solve the problems of early cell apoptosis, slow division and growth, etc., and achieve the effect of increased number of surviving cells, strong vitality, and fast growth rate

Inactive Publication Date: 2014-04-02
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

"Journal of Guangzhou University of Traditional Chinese Medicine" published an article entitled "Study on the Primary Culture and Sterilization Method of Trichosantherea Cells" in Volume 28, No. 3, May 2011. Although the culture and sterilization method is also a common animal cell culture method, it is found in the culture process and subsequent observation that it has the defects of slow cell division and growth during passage, and is prone to early apoptosis defects.

Method used

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  • Intestinal epithelial cell line of pheretima aspergillum and construction method for intestinal epithelial cell line
  • Intestinal epithelial cell line of pheretima aspergillum and construction method for intestinal epithelial cell line
  • Intestinal epithelial cell line of pheretima aspergillum and construction method for intestinal epithelial cell line

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0028] Example 1 (Establishment of the intestinal epithelial cell line of Vermicularis paniculata)

[0029] 1. Reagents:

[0030] (1) Antibiotic combination solution: This combination solution is made by adding penicillin sodium, streptomycin sulfate and gentamicin sulfate to the LBSS solution and mixing. In the prepared antibiotic combination solution, the concentration of penicillin sodium is 200U / ml, the concentration of streptomycin sulfate is 400 μg / ml, the concentration of gentamicin sulfate is 300 U / ml, and the concentration of amphotericin B is 5 μg / ml.

[0031] (2) Thermolysin enzymatic hydrolysis solution: The enzymatic hydrolysis solution is made by adding Thermolysin powder to the HEPES solution with a concentration of 10 m mol / ml, filtering, sterilizing and mixing. The concentration of Thermolysin in the resulting Thermolysin hydrolysis solution is 25 μg / mL.

[0032] (3) Collagenase Type I enzymatic hydrolysate: The enzymatic hydrolysate is made by adding Collagenase T...

example 2

[0048] Example 2 (Effects of the intestinal epithelial cell line of Earthworm ginseng)

[0049] The samples and reference substances used in this example are as follows:

[0050] Sample: The intestinal epithelial cell line of P. serrata obtained in Example 1.

[0051] Reference substance: the method described in the article titled "Study on the method of primary culture and sterilization of the cells of P. paniculata" was published in the "Journal of Guangzhou University of Traditional Chinese Medicine", Volume 28, May 2011. Intestinal epithelial cell line.

[0052] 1. Count of viable cells:

[0053] (1) Reagents:

[0054] (1.1) Trypan blue staining solution: This staining solution is prepared by adding trypan blue powder into double-distilled water to form a 40g / L dyeing mother solution, which is diluted to 4g / L by adding PBS during use.

[0055] (1.2) MTT reagent: This reagent is to add MTT powder to the complete solution of PBS, filter with 0.22umL membrane to form MTT reagent with a ...

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Abstract

The invention relates to an intestinal epithelial cell line of pheretima aspergillum. The intestinal epithelial cell line is characterized in that the collection number of the intestinal epithelial cell line is CCTCC-C201294. The population of cells survived in the intestinal epithelial cell line of pheretima aspergillum is high, the cells are high in vitality, and the cell line is high in splitting and growth speed in the subculturing process and can be used for developing medicines or nutrient and health-care products.

Description

Technical field [0001] The invention relates to undifferentiated non-human cells, in particular to an earthworm intestinal epithelial cell line. Background technique [0002] Pheretima aspergillum (E. Perrier) comes from the genus Pheretima aspergillum, and is one of the four original animals included in the "Earth Dragon" section of the 2010 edition of the Pharmacopoeia of the People's Republic of China. Because it is mainly produced in Guangdong and Guangxi, it is called "Guangdilong". Due to its definite curative effect and meticulous processing of medicinal materials, it is recognized as the best quality in the industry. The varieties of Chinese medicine Dilong included in the Pharmacopoeia of the People’s Republic of China also include the dry body of Phetima pectinifera (Michaelsen), Pheretima guillelmi (Michaelsen), and the popular Pheretimav μlgaris (Chen). Called "Shanghai Dragon". Earthworm is cold in nature and salty in taste. It is returned to the liver, spleen and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/07
Inventor 李薇侯雪芹林小桦喻良文卢瑞珊吴文如
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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