Probiotics and vitamin premix and mixture for middle pig
A technology of vitamin premixes and prebiotics, which is applied in applications, animal feeds, animal feeds, etc., can solve problems such as unsatisfactory effects, insufficient nutritional levels, unreasonable ratios of compound vitamins, etc., to improve animal production performance and improve Digestibility, immune function enhancement effect
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Embodiment 1
[0034] The preparation of each probiotic powder of embodiment 1
[0035] 1. Preparation of Bacillus subtilis CGMCC No.6511 bacterial powder
[0036] 1) Preparation of Bacillus subtilis seed solution: Inoculate the strains preserved on the slant of the test tube into a test tube containing 15 mL of culture medium, and cultivate in a constant temperature shaker at 37°C for 16-20 hours at a speed of 225 rpm until the number of viable bacteria reaches 10 7 CFU / mL, inoculate at 1% in a 250mL Erlenmeyer flask containing 100mL seed medium, culture in a constant temperature shaker at 37°C for 16-20h, the shaker speed is 225rpm, until the number of viable bacteria reaches 10 7 CFU / mL, used as seed solution for later use.
[0037] The seed medium is: glucose, 20.0g / L; peptone, 10.0gL; yeast powder, 4.0g / L; sodium chloride, 5gL; distilled water added to 1000mL, pH 7.0±0.2.
[0038] 2) Liquid submerged fermentation culture
[0039] A 5L vertical fermenter was selected for liquid fermen...
Embodiment 2
[0067] Every kilogram of prebiotic vitamin premix for pigs in the present invention consists of the following components:
[0068] 0.8% compound multi-dimensional;
[0069] 13.0% probiotic powder (Lactobacillus acidophilus: Bacillus subtilis=1:1);
[0070] 7.0% sodium butyrate preparation (sodium butyrate content is 30%);
[0071] 79.2% is corncob flour.
[0072] Among them, the compound multidimensional components contained in each kilogram of prebiotic vitamin premix for pigs are: vitamin A, 350,000 IU; vitamin D3, 100,000 IU; vitamin E, 1000 IU; vitamin K3, 216mg; vitamin B1, 72mg; Vitamin B2, 216mg; Vitamin B6, 108mg; Vitamin B12, 0.72mg; Niacin, 1440mg; Pantothenic acid, 900mg; Folic acid, 47.8mg; Biotin, 2.88mg;
[0073] The Lactobacillus acidophilus bacteria powder and the Bacillus subtilis bacteria powder prepared in the probiotic bacteria powder were prepared from Example 1.
Embodiment 3
[0075] Every kilogram of prebiotic vitamin premix for pigs in the present invention consists of the following components:
[0076] 1.0% composite multi-dimensional;
[0077] 12.0% Lactobacillus acidophilus powder;
[0078] 5.0% sodium butyrate preparation (sodium butyrate content is 30%);
[0079] 82.0% is a mixture of rice bran and corn cob flour (1:1).
[0080] Among them, the compound multidimensional components contained in each kilogram of prebiotic vitamin premix for pigs are: vitamin A, 400,000 IU; vitamin D3, 125,000 IU; vitamin E, 1250 IU; vitamin K3, 240mg; vitamin B1, 80mg; Vitamin B2, 240mg; Vitamin B6, 120mg; Vitamin B12, 0.8mg; Niacin, 1600mg; Pantothenic acid, 1000mg; Folic acid, 52mg; Biotin, 3.2mg;
[0081] Wherein the Lactobacillus acidophilus bacteria powder prepared in the probiotic bacteria powder is prepared from Example 1.
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