Rabdosia rubescens liquid extract and preparation method and application thereof

A technology of rubescens leaf and water extract, applied in the field of biomedicine, can solve the problems of increasing the risk of breast cancer, increasing the risk of venous thromboembolic diseases, softening breasts and the like

Active Publication Date: 2013-02-06
北京恒润泰生医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although estrogen is by far the most effective drug for treating osteoporosis in postmenopausal women, long-term use of hormone replacement therapy or estrogen replacement therapy will inevitably cause some complications, such as a significant decline in the bone formation surface,

Method used

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  • Rabdosia rubescens liquid extract and preparation method and application thereof
  • Rabdosia rubescens liquid extract and preparation method and application thereof
  • Rabdosia rubescens liquid extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The preparation of embodiment 1 oridonium leaf water extract

[0024] After washing 21g of dried Radix japonicus leaves with tap water, add 500ml of double distilled water and stir and extract at 80°C for 30 minutes. The clear liquid obtained by filtration was concentrated under reduced pressure at 40° C. to obtain 6.3 g of water extract of Radix radix leaves as a pale yellow solid. Grind into powder for later research use.

Embodiment 2

[0025] The HPLC-diode array fingerprint of embodiment 2 Radix radix leaf water extract

[0026] HPLC: Waters 2695Separations Module;

[0027] Detector: Waters 2996 diode array (PAD);

[0028] Kromasil C 18 Reversed-phase column (5μm, 250mm×4.6mm);

[0029] Guard column (5μm, 10mm×4.6mm);

[0030] Mobile phase: acetonitrile / water (containing 0.05% glacial acetic acid);

[0031] Mobile phase flow rate: 0.3ml / min;

[0032] Injection volume: 50μl;

[0033] Elution method: gradient elution (see Table 1 for the specific gradient)

[0034] Table 1 Gradient table of mobile phase for HPLC-diode array analysis of the water extract of Rubescens japonicus

[0035]

[0036] The 3D map of the chromatogram-ultraviolet absorption spectrum of the water extract of Dong Lingcao: see figure 1 .

[0037] The HPLC fingerprint of the water extract of Rubescens japonicus under 254nm detection: see figure 2 , 39 peaks were analyzed at one time, and the retention times of 30 peaks are lis...

Embodiment 3

[0040] Example 3 (-)ESI-Q-TOF-MS fingerprints of the water extract of Radix radix leaves

[0041] (-)ESI-Q-TOF-MS: using an optimized small molecule analysis method, the specific parameters are a) Source Type: ESI; b) Ion Polarity: Negative; c) Scan begin: 50m / z; d) Scan begin: 1500m / z; e) Set Capillary: 3500 V; f) Set Nebulizer: 0.8Bar; g) Set Dry Heater: 200℃; h) Set Dry Gas: 8.0ml / min; i) Funnel 1 RF: 300Vpp; j) Funnel 2RF: 300Vpp; k) ISCID Energy: 0eV; l) Hexapole RF: 200Vpp; m) Ion Energy: 5eV; n) Low Mass: 200m / z; o) Collision Energy: 14eV; p) Collision RF: 140Vpp ; q) Transfer Time: 110.8 μS; r) Pre Puls Storage: 8.7 μS; s) Source: 1400v, 2nA; t) Calibrate the mass spectrometer with sodium formate before each analysis.

[0042]Using the HPLC-diode array analysis and the well-explored high-performance liquid chromatography conditions and the system-optimized mass spectrometry conditions, the negative ion mode (-)ESI was used to conduct (-)ESI-Q-TOF-MS on the water extra...

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PUM

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Abstract

The invention relates to a rabdosia rubescens liquid extract and a preparation method thereof. The preparation method includes cleaning dry rabdosia rubescens with running water, adding double distilled water according to the proportion of 1:(20-28) (g:ml) and stirring and extracting for 10-60 minutes at the temperature of 60-100 DEG C, filtering to obtain clean liquid, concentrating the clean liquid at the temperature of 40 DEG C on a decompressing condition, and finally obtaining the dry powder of the rabdosia rubescens liquid extract. The invention further provides an analysis method of the rabdosia rubescens liquid extract, and further relates to application of the rabdosia rubescens liquid extract on ovariectomized rat model as an anti-osteoporosis agent.

Description

field of invention [0001] The present invention relates to the water extract of Boswellia leaves and its preparation method, to the HPLC-diode array / (-)ESI-MS-MS fingerprint of the water extract, and to the evaluation of the water extract on the ovariectomized female mouse model. Extract as an anti-osteoporosis agent. The invention belongs to the field of biomedicine. Background technique [0002] Osteoporosis is a multifactorial metabolic bone disease characterized by diffuse bone loss. More than 50% of postmenopausal women over the age of 50 experience fractures caused by osteoporosis at some point in their lives. Among the women with fractures caused by osteoporosis, more than 50% will take a long time to recover, which will inevitably lead to some sequelae including death. It is estimated that at least 20% of women who experience a fracture die. Preliminary surveys in Beijing and Shanghai show that the prevalence of osteoporosis in women over 60 years old is 40-50%. ...

Claims

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Application Information

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IPC IPC(8): A61K36/53A61P19/10A61K127/00
Inventor 彭师奇赵明彭莉姚清河
Owner 北京恒润泰生医药科技有限公司
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