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Composite dressing of pig accellular dermal matrix and preparation method of composite dressing

An acellular dermis and collagen technology, applied in the field of medical skin tissue engineering, can solve the problems of collagen and matrix membrane damage, time-consuming and labor-intensive, and complicated processing procedures.

Inactive Publication Date: 2013-02-13
GUANGZHOU TRAUER BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It makes the processing process more complicated, and the processing time is relatively long. Compared with other chemical methods, the processing time is too long, time-consuming and labor-intensive.
And the balanced salt solution method will cause considerable damage to the collagen and matrix membrane in the dermis
[0008] The enzymatic digestion method also has defects such as complicated operation and difficult control

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for preparing a composite dressing of porcine acellular dermal matrix coated with collagen includes the following steps.

[0034] (1) Washing, specifically washing the skin sheet in PBS phosphate buffered saline solution.

[0035] (2) Separate the epidermis and dermis, specifically by soaking and digesting the skin sheet obtained in step (1) in DispaseII solution, decomposing type IV collagen and fibronectin, separating epidermis and dermis, and then washing in PBS phosphate buffered saline solution .

[0036] (3) Dedermal cells, specifically, the skin sheet obtained in step (2) is soaked in TritonX-100 solution to further remove dermal cell components, and then washed in PBS phosphate buffered saline solution.

[0037] (4) Ultrasonic cleaning, specifically, ultrasonic cleaning of the skin sheet obtained in step (3), and then soaking in PBS phosphate buffered saline solution for temporary storage.

[0038] (5) Collagen coating, specifically, the skin sheet obtained in s...

Embodiment 2

[0042] A method for preparing a composite dressing of porcine acellular dermal matrix coated with collagen specifically includes the following steps.

[0043] (1) Wash, wash the skin piece in PBS phosphate buffered saline solution for 10-15 minutes.

[0044] (2) Separate the epidermis and dermis, specifically by immersing the skin sheet obtained in step (1) in a Dispase II solution with a concentration of 2.0-3.0 U / mL, and digesting it at 3-6°C for 40-60 hours to decompose Type IV Collagen and fibronectin are separated from epidermis and dermis; then washed in PBS phosphate buffered saline solution for 10-15 minutes to clean.

[0045] (3) Dedermal cells, specifically, the skin sheet obtained in step (2) is soaked in a 0.3-0.8% TritonX-100 solution for 40-60 hours to further remove the dermal cell components, and then immerse it in PBS phosphate buffer Wash in salt solution for 10-15 minutes.

[0046] (4) Ultrasonic cleaning, specifically, placing the skin sheet obtained in step (3) i...

Embodiment 3

[0050] A method for preparing a composite dressing of porcine acellular dermal matrix coated with collagen includes the following steps.

[0051] (1) Wash, wash the skin piece in PBS phosphate buffered saline solution for 10-15 minutes.

[0052] (2) Separate the epidermis and dermis, specifically by immersing the skin sheet obtained in step (1) in a Dispase II solution with a concentration of 2.5 U / ml, and digesting it at 4°C for 48 hours to decompose type IV collagen and fibronectin. Separate the epidermis and dermis; then wash in PBS phosphate buffered saline solution for 10-15 minutes to clean.

[0053] (3) Dedermal cells, specifically, the skin sheet obtained in step (2) is soaked in 0.5% TritonX-100 solution for 48 hours to further remove dermal cell components, and then washed in PBS phosphate buffered saline solution 10-15 minutes.

[0054] (4) Ultrasonic cleaning, specifically, placing the skin sheet obtained in step (3) in an ultrasonic cleaning machine, shaking and washing ...

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PUM

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Abstract

The invention relates to a composite dressing of a pig accellular dermal matrix and a preparation method of the composite dressing. The preparation method comprises the following steps: (1) washing: washing a skin piece in a phosphoric acid buffer salt solution; (2) separating epidermis and dermis: soaking and digesting the skin piece in a Dispase II solution to be decomposed into IV type collagen and fiber mucoprotein, separating epidermis and dermis, and then washing in the phosphoric acid buffer salt solution; (3) removing dermal cells, namely, soaking the skin piece in a Triton X-100 solution to further remove dermal cell components, and then washing in the phosphoric acid buffer salt solution; (4) carrying out ultrasonic washing, and then soaking in the phosphoric acid buffer salt solution for temporary storage; and (5) coating collagen: soaking the skin piece in I type collagen for filling and sealing, or soaking the skin piece in the I type collagen for freeze drying, sealing and packaging freeze dried substances to obtain finished products. According to the invention, cells are thoroughly removed, and the composite dressing is complete in fiber support, strong in applicability and good in application effect.

Description

technical field [0001] The invention relates to the technical field of medical skin tissue engineering, in particular to a collagen-coated composite dressing of porcine acellular dermal matrix and a preparation method thereof. [0002] Background technique [0003] In recent years, with the development of industry, personal accident injuries are inevitably increasing. In addition, people's requirements for their own image in life and work are also increasing, so more and more people need skin repair. [0004] In the prior art, in the field of wound repair, it has been found that the dermis can promote tissue repair and reduce the formation of scars during the healing process. Therefore, the selection, extraction, preparation and application of dermal tissue has always been a research hotspot in the field of skin repair. [0005] However, since the dermal tissue is prone to a series of immune rejection during the repair process, the cause of the immune rejection mainly comes...

Claims

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Application Information

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IPC IPC(8): A61L15/40A61L15/32
Inventor 迟妍妍雷静钟翠红盛晓晓黄冬晶
Owner GUANGZHOU TRAUER BIOTECH
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