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Helicobacter pylori immunodominance epitope peptide and preparation method and application thereof

A Helicobacter pylori, dominant epitope technology, applied in the field of medical biology, can solve problems such as self-reactive epitopes cannot be ruled out

Inactive Publication Date: 2013-02-13
中国人民解放军第三军医大学药学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mass spectrometry is simple, time-saving and fast, but cannot exclude self-reactive epitopes and epitopes that cannot be processed and presented by antigen-presenting cells in their natural state
Although the software prediction method is simple and fast, the prediction results are often different from the real situation, which needs to be verified by experiments

Method used

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  • Helicobacter pylori immunodominance epitope peptide and preparation method and application thereof
  • Helicobacter pylori immunodominance epitope peptide and preparation method and application thereof
  • Helicobacter pylori immunodominance epitope peptide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: Step-overlap synthesis of 18 amino acid short peptides and preparation of mixed peptide library

[0046] Helicobacter pylori urease protein B subunit (UreB) has a full length of 569 amino acids, and its sequence is conserved among various strains. The recombinantly constructed Helicobacter pylori urease B subunit is a 1-569 polypeptide, which is derived from the 11637 international standard strain. The amino acid sequence of the B subunit of urease protein derived from Helicobacter pylori 11637 (number P69996) was retrieved from the UniProt protein database, and a short peptide of 18 amino acids was synthesized by stepping and overlapping from the first amino acid (synthesized with the assistance of Shanghai Jier Biochemical Company), a total of 93 strips (the last one is a 17 amino acid short peptide). The purity is greater than 70%. The synthetic peptide information is shown in Table 1. The synthesized peptides were dissolved in DMSO to a storage concen...

Embodiment 2

[0056] Example 2: Evaluation of immune effect and analysis of antibody response in different adjuvant groups

[0057] 1. Experimental protocol for animal immunization and challenge

[0058] Mice were immunized with rUreB antigen and different adjuvants. The specific immunization plan is as follows:

[0059] Experimental animals: BALB / c female mice aged 6-8 weeks.

[0060] Immunization methods: nasal drops, subcutaneous.

[0061] Immunization volume: intranasal, 8ul / monkey; subcutaneous, 200ul / monkey.

[0062] Experimental group:

[0063] ① rUreB (100ug / cause) + Freund's adjuvant (volume 1:1). Subcutaneous immunization 3 times (week 0, 2, 4).

[0064] ②rUreB (50ug / piece) + CpG (20ug / piece). Intranasal immunization 4 times (1st, 2nd, 3rd, 4th week).

[0065] ③rUreB (100ug / piece) + CpG (20ug / piece). Subcutaneous immunization 3 times (week 0, 2, 4).

[0066] ④ rUreB (100ug / piece) + AddaVax (volume 1:1). Subcutaneous immunization 3 times (week 0, 2, 4).

[0067] ⑤ rUr...

Embodiment 3

[0077] Example 3: Collection and Preservation of Lymphocytes in Mouse Spleen

[0078] The mouse spleen was isolated under aseptic conditions, ground, and made into a single cell suspension, and the buffy coat lymphocytes were collected after density gradient centrifugation of mouse lymphocyte separation medium. The isolated splenic lymphocytes were not used for in vitro culture, and the excess cells were adjusted to a cell concentration of 1×10 with cryopreservation solution. 7 / mL, add 1mL / tube into a cryopreservation tube, put it into a freezer box at -70°C overnight, and transfer it to liquid nitrogen for cryopreservation.

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Abstract

The invention relates to helicobacter pylori immunodominance epitope peptide and a preparation method and the application thereof. The amino acid sequence of the dominance epitope peptide is shown as SEQ ID NO: 97, 100, 108 and 115. A preparation method and application of the dominance epitope peptide in prevention or treatment of helicobacter pylori infection are further provided.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and relates to a helicobacter pylori immunodominant epitope peptide and a preparation method and application thereof. Background technique [0002] Helicobacter pylori (Hp) was discovered by Australian scholars Warren and Marshall in 1982. It colonizes in the local human gastric mucosa, causing more than 50% of the world's population to infect, becoming chronic gastritis, gastroduodenal ulcer and gastric mucosa-related lymphoid The main pathogenic factor of gastrointestinal diseases such as tissue lymphoma. At the same time, the World Health Organization has listed Hp as a kind of carcinogenic factor closely related to the occurrence of gastric cancer. At present, the multi-drug therapy of antibiotics and proton pump inhibitors is mainly used clinically for H. Vaccine immunization may become an effective means to completely eliminate Hp infection and treat related gastrointestinal ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61K39/02A61P31/04
Inventor 吴超邹全明李滨陈立杨武晨李海波章金勇赵卓
Owner 中国人民解放军第三军医大学药学院
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