Extraction and refinement method for microbial oil
A microbial oil and microbial technology, applied in the direction of fat oil/fat refining, fat production, fat oil/fat production, etc., can solve the problem of reducing the content of polyunsaturated fatty acids in microbial oil, rare in industrial production, and the extraction effect is not optimistic, etc. problem, to avoid oxidative deterioration
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Embodiment 1
[0034] pre-fermentation
[0035] 1. Activation of the original strain: inoculate the Mortierella alpina strain preserved in an amber tube on a PDA slant medium, culture it at 28±1°C for 8 days, select a PDA medium plate with vigorous growth of hyphae and spores, and remove it. The hyphae and spores on the culture medium are made into spore suspension with sterile water;
[0036] 2 Seed culture: inoculate the spore suspension in the shake flask culture medium, shake and culture at 28±1°C for 48 hours, the shaking speed of the shaker is 200±20 rpm, the mass of the main raw material components in the liquid culture medium The percentage is glucose 4%, peptone 1%, yeast extract 3%, matrix distilled water, pH value is 7.2;
[0037] 3 Expanded seed cultivation: final fermentation tank volume is 50m 3 , so the selected seed pots are 10m 3 、1m 3 , inoculate 1.5L of shake flask seed fermentation liquid in step (2) to 1m 3 In the seed expansion fermenter, the mass percentages of th...
Embodiment 2
[0080] pre-fermentation
[0081] 1. Activation of the original strain: inoculate the Mortierella alpina strain preserved in an amber tube on a PDA slant medium, culture it at 28±1°C for 9 days, select a PDA medium plate with vigorous growth of hyphae and spores, and remove it. The hyphae and spores on the culture medium are made into spore suspension with sterile water;
[0082] 2 Seed culture: inoculate the spore suspension in the shake flask culture medium, shake and culture at 28±1°C for 48 hours, the shaking speed of the shaker is 200±20 rpm, the mass of the main raw material components in the liquid culture medium The percentage is glucose 5%, peptone 1.5%, yeast extract 2%, matrix distilled water, pH value is 7.0;
[0083] 3 Expanded seed cultivation: final fermentation tank volume is 200m 3 , so the seed tanks are 50m respectively 3 、5m 3 , 500L, inoculate 1L shake flask seed fermentation liquid in the seed expansion fermenter of 500L, cultivate 24h under the temper...
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