Extraction and refinement method for microbial oil

A microbial oil and microbial technology, applied in the direction of fat oil/fat refining, fat production, fat oil/fat production, etc., can solve the problem of reducing the content of polyunsaturated fatty acids in microbial oil, rare in industrial production, and the extraction effect is not optimistic, etc. problem, to avoid oxidative deterioration

Active Publication Date: 2013-02-13
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many methods for leaching and extracting microbial oils, including the method of pressing mixed plant seeds mentioned in the Chinese patent with the publication number CN101133144A. Since the microbial cells are single cells, the individual pressure-bearing capacity is extremely strong, and it is difficult to squeeze only by pressing. The microbial oil in the microbial cells is squeezed out, so the patent uses a solvent leaching process in the later stage, but the operation process is too complicated, and at the same time plant seeds are added to reduce the content of polyunsaturated fatty acids in the microbial oil; the Chinese publication number is CN1447860A The patent proposes to collect microbial cells and dissolve the cell wall by enzymatic technology to separate microbial oils. Although this method can not use solvents, the extraction effect is not optimistic; CN1279154C mentions the use of carbon dioxide supercritical extraction technology to extract microbial oils, and At present, there are many reports on the application of supercritical extraction technology in the laboratory, but there are not many reports that can be applied to industrial production.
[0004] In addition, there is currently no research on microbial oil refining technology.

Method used

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  • Extraction and refinement method for microbial oil
  • Extraction and refinement method for microbial oil
  • Extraction and refinement method for microbial oil

Examples

Experimental program
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Effect test

Embodiment 1

[0034] pre-fermentation

[0035] 1. Activation of the original strain: inoculate the Mortierella alpina strain preserved in an amber tube on a PDA slant medium, culture it at 28±1°C for 8 days, select a PDA medium plate with vigorous growth of hyphae and spores, and remove it. The hyphae and spores on the culture medium are made into spore suspension with sterile water;

[0036] 2 Seed culture: inoculate the spore suspension in the shake flask culture medium, shake and culture at 28±1°C for 48 hours, the shaking speed of the shaker is 200±20 rpm, the mass of the main raw material components in the liquid culture medium The percentage is glucose 4%, peptone 1%, yeast extract 3%, matrix distilled water, pH value is 7.2;

[0037] 3 Expanded seed cultivation: final fermentation tank volume is 50m 3 , so the selected seed pots are 10m 3 、1m 3 , inoculate 1.5L of shake flask seed fermentation liquid in step (2) to 1m 3 In the seed expansion fermenter, the mass percentages of th...

Embodiment 2

[0080] pre-fermentation

[0081] 1. Activation of the original strain: inoculate the Mortierella alpina strain preserved in an amber tube on a PDA slant medium, culture it at 28±1°C for 9 days, select a PDA medium plate with vigorous growth of hyphae and spores, and remove it. The hyphae and spores on the culture medium are made into spore suspension with sterile water;

[0082] 2 Seed culture: inoculate the spore suspension in the shake flask culture medium, shake and culture at 28±1°C for 48 hours, the shaking speed of the shaker is 200±20 rpm, the mass of the main raw material components in the liquid culture medium The percentage is glucose 5%, peptone 1.5%, yeast extract 2%, matrix distilled water, pH value is 7.0;

[0083] 3 Expanded seed cultivation: final fermentation tank volume is 200m 3 , so the seed tanks are 50m respectively 3 、5m 3 , 500L, inoculate 1L shake flask seed fermentation liquid in the seed expansion fermenter of 500L, cultivate 24h under the temper...

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Abstract

The present invention relates to an extraction and refinement method for microbial oil. The method is characterized by comprising the following steps: (1) carrying out a post-treatment on a fermentation broth: after completing fermentation, carrying out a high temperature short time inactivation treatment on a fermentation broth, carrying out bacterial liquid separation to obtain wet bacterial, and drying; (2) carrying out microbial oil extraction: extracting microbial oil under a temperature condition of an extraction temperature of not more than 60 DEG C to obtain mixed oil, cooling to a temperature of -10 to 10 DEG C, filtering to remove impurities and colloid substances in the mixed oil, and carrying out vacuum desolution to obtain crude oil; and (3) carrying out refinement: carrying out alkali refinement, adding active white clay to the alkali refined system to carry out decoloration, adding silicon dioxide and active carbon to carry out absorption on hazards, filtering to remove the active white clay, the silicon dioxide and the active carbon, and carrying out deordorization at a temperature of 180-190 DEG C to obtain the finished product oil. The extraction and refinement method is suitable for microbial oil, and is an extraction and refinement method for effectively reducing hazard content in the oil in industrial production.

Description

technical field [0001] The invention relates to a method for extracting and refining microbial oil. Background technique [0002] Long-chain polyunsaturated fatty acids are closely related to human nutrition. Although adults can synthesize long-chain unsaturated fatty acids such as arachidonic acid and docosahexaenoic acid through precursors, for infants and young children, their synthesis ability is still lacking, so it is necessary to supplement arachidonic acid and docosahexaenoic acid in time. Docosahexaenoic acid and other long-chain unsaturated fatty acids to promote the development of the brain and retina of infants. To this end, the Joint Committee of the World Food and Agriculture Organization and the World Health Organization proposed adding arachidonic acid and docosahexaenoic acid to infant formula. [0003] At present, there are many methods for leaching and extracting microbial oils, including the method of pressing mixed plant seeds mentioned in the Chinese ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/02C11B1/10C11B1/00C11B3/06C11B3/10C11B3/00
Inventor 唐孝鹏汪志明周强刘宏荣
Owner CABIO BIOTECH WUHAN CO LTD
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