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Compound bacterium capable of degrading kitchen waste and preparation method of compound bacterium

A technology for kitchen waste and compound bacteria, which is applied in biochemical equipment and methods, microorganism-based methods, bacteria, etc., can solve the problems of high equipment and operating costs, increased processing costs, and large footprint, and achieves strong performance. Adaptability, inhibition of fungal diseases, inhibition of growth of pathogenic bacteria

Inactive Publication Date: 2013-02-13
北京世纪阿姆斯生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the main disadvantages of large and medium-sized treatment are: adding a large amount of moisture regulator to increase the treatment cost, easy to generate stench, and occupy a large area
Small-scale in-situ treatment technologies mostly use high-temperature processes at 60-80°C, and their main disadvantages are: small processing capacity, high equipment and operating costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] High-density culture of Bacillus subtilis, Saccharomyces cerevisiae, Aspergillus oryzae, Sporosporium thermophila and Geotrichum candida:

[0038] (1) Single bacterial agent adsorption carrier: rice bran, corncob powder, and bran are mixed evenly according to the mass ratio of 1:0.5:1, and used as the adsorption carrier of the bacterial agent.

[0039] (2) Bacillus subtilis: inoculate the Bacillus subtilis bacterial classification on the slant medium of purified culture to the shake flask that is equipped with the activation medium of 15% by volume ratio, and the formula of described activation medium is glucose 15g / L, peptone 10g / L, sodium chloride 3g / L and beef extract 0.3g / L were activated and cultivated in a shake flask with a culture temperature of 37°C and a shake flask rotation speed of 170rpm for 24 hours; It is in the fermenter that the volume ratio of seed inoculation is housed is 50% fermentation medium, and the volume inoculation amount is 1%, and the formu...

Embodiment 2

[0046] High-density culture of Bacillus subtilis, Saccharomyces cerevisiae, Aspergillus oryzae, Sporosporium thermophila and Geotrichum candida:

[0047] (1) Single bacterial agent adsorption carrier: rice bran, corn cob powder, and bran are mixed evenly according to the mass ratio of 3:1:3, and used as the adsorption carrier of the bacterial agent.

[0048] (2) Bacillus subtilis: inoculate the Bacillus subtilis bacterial classification on the slant culture medium of purifying culture to the shake flask that is equipped with the activation medium of 30% by volume ratio, and the formula of described activation medium is glucose 20g / L, peptone 15g / L, sodium chloride 7g / L, and beef extract 0.8g / L were activated and cultivated in a shake flask with a culture temperature of 37°C and a shake flask rotation speed of 180rpm for 24 hours; the activated and cultured Bacillus subtilis bacteria It is in the fermenter that the volume ratio of seed inoculation is housed is 70% fermentation...

Embodiment 3

[0055] High-density culture of Bacillus subtilis, Saccharomyces cerevisiae, Aspergillus oryzae, Sporosporium thermophila and Geotrichum candida:

[0056] (1) Single bacterial agent adsorption carrier: rice bran, corncob powder, and bran are mixed uniformly according to the mass ratio of 2:0.5:2, and used as the bacterial agent adsorption carrier.

[0057] (2) Bacillus subtilis: inoculate the Bacillus subtilis bacterial classification on the slant medium of purified culture to the shake flask that is equipped with the activation medium of 20% by volume ratio, and the formula of described activation medium is glucose 16g / L, peptone 12g / L, sodium chloride 4g / L and beef extract 0.5g / L were activated and cultivated in a shake flask with a culture temperature of 37°C and a shake flask rotation speed of 170rpm for 24 hours; It is in the fermenter that the volume ratio of seed inoculation is equipped with 60% fermentation medium, and the volume inoculation amount is 2%, and the formu...

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PUM

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Abstract

The invention discloses a compound bacterium capable of degrading kitchen waste and a preparation method of the compound bacterium. The preparation method comprises the following steps that strains of bacillus subtilis, saccharomyces cerevisiae, aspergillus, thermophilic sporotrichum and geotrichum candidum are cultured at a high density respectively, and then inoculated to shake flasks containing culture media with the volume ratios of 15-30% for active culture; the cultured strains are inoculated to fermentation tanks containing fermentation culture media with the volume ratios of 50-70% for fermentation enlarging culture; the cultured strains are absorbed by carriers respectively to be prepared into dried single microbial inoculums; and the following single microbial inoculums in parts by weight: 15-30 parts of bacillus subtilis single microbial inoculum, 10-25 parts of saccharomyces cerevisiae single microbial inoculum, 10-30 parts of aspergillus single microbial inoculum, 10-30 parts of thermophilic sporotrichum single microbial inoculum and 10-30 parts of geotrichum candidum single microbial inoculum, are mixed. The compound bacterium and the preparation method have the benefits of low productive investment, low manufacturing cost, convenience in use and good degrading effect.

Description

technical field [0001] The invention relates to the technical field of microbial bacterial agents, in particular to a food waste-degrading composite bacterium and a preparation method thereof. Background technique [0002] With the rapid development of social economy and the acceleration of urbanization process, the generation of urban domestic waste in my country continues to increase. Kitchen waste accounts for about 37% to 62% of domestic waste. Food waste contains a lot of sugar, starch, protein, and high salt and oil content. It is easy to rot and deteriorate, emits bad smell, spreads bacteria and viruses, and breeds mosquitoes. Flies have become the main pollutants in the city environment. "Gutter oil" and "garbage pigs" caused by kitchen waste have become public hazards in urban life. But from another point of view, food waste is rich in nutrients, high in organic matter and inorganic salt content, and can become a resource with certain value if it can be treated re...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18C12N1/14C12N11/02B09B3/00C12R1/125C12R1/865C12R1/69C12R1/645
Inventor 李春龙
Owner 北京世纪阿姆斯生物技术有限公司