Method for detecting content of ciprofloxacin

A technology of ciprofloxacin hydrochloride and detection method, applied in material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of sensitivity, stability distance, etc., and achieve the effects of good reproducibility, rapid analysis, and simple operation

Active Publication Date: 2013-02-13
HAINAN HERUI PHARMA
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Problems solved by technology

At present, people try to establish methods for detecting the content of ciprofloxacin hydrochloride by using spectrophotometry, fluorescence photometry, and electroanalysis methods. There is still a certain distance between the requirements of practical application

Method used

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Embodiment

[0012] (1) Under stirring conditions, slowly add 40 mL of anhydrous ethanol solution containing 10 mmol of aminothiophenol to 20 mL of anhydrous ethanol solution containing 10 mmol of terephthalaldehyde, and heat it in a water bath at 60°C for 4 hours to obtain Bright yellow precipitate, recrystallized with absolute ethanol, and dried in vacuum to obtain Schiff base powder of terephthalaldehyde condensed with o-aminothiophenol.

[0013] (2) Weigh out 0.002g of the Schiff base powder of terephthalaldehyde and o-aminothiophenol obtained in step (1), stir and dissolve it in 10 mL ultrapure water at room temperature, and configure it into terephthalaldehyde and o-aminobenzene. Thiophenol Schiff base aqueous solution, then pipette 50μL of this solution and slowly add it to 100μL of 1×10 -3 In mol / L manganese chloride aqueous solution, mix evenly to obtain the fluorescent probe solution.

[0014] (3) Place 50 μL of the fluorescent probe solution obtained in step (2) in 1 mL of HA with pH...

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Abstract

The invention discloses a method for detecting the content of ciprofloxacin, which comprises the steps of: mixing a ciprofloxacin solution and a fluorescent probe solution, detecting the fluorescent intensity of a mixture, calculating a differential value of fluorescence intensity before and after the mixing, and obtaining the content of ciprofloxacin according to the differentia value and a preset standard curve. The method provided by the invention applies the fluorescent probe solution, the fluorescent probe consists of a terephthalaldehyde-o-aniline thiophenol Schiff base solution and a manganese chloride aqueous solution, and the content of ciprofloxacin is detected through a fluorescence spectrophotometry due to the fact that the quenching function of ciprofloxacin on the fluorescent light of the fluorescent probe solution and positive correlation between the degree of the fluorescent quenching and the concentration of ciprofloxacin. The method provided by the invention is simple to operate, fast to analyze, stable in system and good in reproducibility.

Description

technical field [0001] The invention relates to a method for rapidly detecting the content of ciprofloxacin hydrochloride by using a fluorescent probe solution. Background technique [0002] Ciprofloxacin hydrochloride is a quinolone antibiotic drug, which has the characteristics of broad spectrum, high efficiency, low toxicity, complete oral absorption, wide distribution in the body, and is not easy to produce drug resistance. It is one of the best anti-infective drugs at present. However, too much ciprofloxacin ingested by humans will lead to excessive levels of transaminases and dehydrogenases in the body, which will bring overloaded absorption to the kidneys, and eventually lead to nephritis, liver necrosis and other diseases. The standard method for detecting the content of ciprofloxacin hydrochloride stipulated in Chinese Pharmacopoeia is high-performance liquid chromatography. High-performance liquid chromatography has the advantages of high sensitivity, high separati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 刘峥袁帅刘洁
Owner HAINAN HERUI PHARMA
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