Preparation method of miniaturized magnetic fluxgate biosensor for serum tumor marker detection

A fluxgate sensor and tumor marker technology, applied in the field of medical detection methods and magnetic sensing, can solve the problems of no miniaturized fluxgate sensor, low sensitivity of individual indicators, lack of flexibility, etc., and achieve no storage requirements, fast detection speed, and high detection sensitivity

Active Publication Date: 2015-04-22
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the shortcomings of these methods are the lack of flexibility, low sensitivity and poor specificity of single indicators.
Through literature and patent searches, no relevant research results were found on the use of miniaturized fluxgate sensors for the detection of serum tumor markers

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The whole preparation process is divided into the following steps:

[0049] (1) The specific production of the miniaturized fluxgate sensor can be realized by using the existing technology, such as the method described in the patent No. 200910046101.1, which will not be repeated here.

[0050] In this embodiment, the miniaturized fluxgate sensor includes a glass substrate, an exciting coil, a receiving coil, a magnetic core, pins, and polyimide insulating material, and two groups of connected three-dimensional magnetic cores are symmetrically wound on a closed rectangular magnetic core. Solenoid excitation coil, and a group of three-dimensional solenoid receiving coils are wound vertically with the excitation coil; the excitation coil is located on the glass substrate, and is formed by connecting the bottom coil and the top coil through connecting conductors, and the two ends of the excitation coil are connected to pins; The coil is located on the glass substrate, and i...

Embodiment 2

[0069] (1) The specific production of the miniaturized fluxgate sensor of the present invention can be realized by using existing technologies, such as the method described in Patent No. 200910046101.1, which will not be repeated here.

[0070] (2) Sputter a Cr / Au thin film on a glass substrate with a thickness of 500nm. Shake the photoresist, dry, expose, develop, etch the Cr / Au film, and remove the glue, so that the Au film is exposed to the outside.

[0071] (3) Preparation of biosensitive film on Au film. The biologically sensitive membrane is a self-assembled membrane with a thickness of nanometer scale, and the self-assembled membrane is formed by 11-mercaptoundecanoic acid and then activated by a mixed solution of EDC and NHS. details as follows:

[0072] ●Au film cleaning: wash with 1M NaOH aqueous solution for 10 minutes, then wash with 1M HCI aqueous solution for 10 minutes, and finally wash with water and absolute ethanol twice, dry at room temperature, and steril...

Embodiment 3

[0088] (1) The specific production of the miniaturized fluxgate sensor can be realized by using the existing technology, such as the method described in the patent No. 200910046101.1, which will not be repeated here.

[0089] (2) Sputter a Cr / Au thin film on a glass substrate with a thickness of 100 nm. Shake the photoresist, dry, expose, develop, etch the Cr / Au film, and remove the glue, so that the Au film is exposed to the outside.

[0090] (3) Preparation of biosensitive film on Au film. The biologically sensitive membrane is a self-assembled membrane with a thickness of nanometer scale, and the self-assembled membrane is formed by 11-mercaptoundecanoic acid and then activated by a mixed solution of EDC and NHS. details as follows:

[0091] ●Au film cleaning: wash with 1M NaOH aqueous solution for 10 minutes, then wash with 1M HCI aqueous solution for 10 minutes, and finally wash with water and absolute ethanol twice, dry at room temperature, and sterilize with ozone ult...

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Abstract

The present invention provides a preparation method of a miniaturized magnetic fluxgate biosensor for serum tumor marker detection. The method comprises the following steps: (1) adopting a MEMS process to prepare a miniaturized magnetic fluxgate sensor on a glass substrate; (2) carrying out Cr / Au film sputtering on the glass substrate, and carrying out spin photoresist coating, exposuring, developing, Cr / Au film etching, and photoresist removing, such that the Au film is exposed; (3) preparing a bio-sensitive film on the Au film; (4) immobilizing tumor marker monoclonal antibody; (5) carrying out antigen spotting; (6) immobilizing biotinylated antibody; and (7) immobilizing a magnetic tag. According to the present invention, the miniaturized magnetic fluxgate biosensor is applied in serum tumor marker detection, the detection is rapid, mass production is easily achieved, and the existing clinical application requirements can be met.

Description

technical field [0001] The invention relates to the technical fields of medical detection methods and magnetic sensing, in particular to a manufacturing method of a miniaturized fluxgate biosensor for detecting serum tumor markers. Background technique [0002] Malignant tumors are one of the major diseases that threaten human health and life today, and the incidence rate is increasing year by year. At present, the main clinical tumor detection methods include: [0003] [1] Laboratory examination: mainly includes routine examination of blood, urine, and stool, biochemical and immune examination, pathological examination, etc. [0004] [2] Radiological examination: mainly including X-ray fluoroscopy, X-ray film, X-ray contrast examination, CT scan, nuclear magnetic resonance imaging, etc. [0005] [3] Radionuclide inspection: that is, isotope inspection, including functional measurement inspection, scanning and gamma irradiation inspection, radioimmunoassay, etc. [0006] ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B81C1/00G01N27/72
Inventor 周勇雷冲雷剑
Owner SHANGHAI JIAO TONG UNIV
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