Preparation of targeted CT (computed tomography) contrast agent of second generation polyamide-amine dendrimer stabilized gold nanoparticle based on folate modification
A technology of amine dendrimers and dendrimers, which is applied in the field of preparation of targeted CT contrast agents, can solve the problems of no targeted CT contrast agents, and achieve enhanced contrast effects, easy operation, and mild preparation process Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0048] Take HAuCl 4 30mg, dissolved in distilled water, magnetic shock to fully dissolve it evenly, and prepare a solution with a concentration of 30mg / mL. Take the second generation polyamide-amine dendrimers (G2.NH 2 ) 150.00mg, dissolved in 15mL distilled water, and magnetically oscillated to fully dissolve and evenly. The solution was preheated in a 60 °C water bath for 20 min. Take HAuCl 4 Add 1.897mL of the solution to the preheated dendrimer solution. The temperature was maintained at 60° C., and the reaction was stirred by magnetic force for 3.0 h. After the reaction, the resulting solution was dialyzed against distilled water (6 times, 2 L / time). Then freeze-drying is carried out to obtain gold nanoparticles stabilized by dendrimers before acetylation, which are stored at -20°C.
Embodiment 2
[0050] Weigh 20.05 mg of folic acid and dissolve it in 5 mL of dimethyl sulfoxide. During magnetic stirring, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride solution (3.483 mL, 12.50 mg / mL) was added, and the reaction was stirred for 3.0 h. Take 100.00 mg of dendrimer-stabilized gold nanoparticles before acetylation prepared in Example 1 and dissolve them in 10 mL of dimethyl sulfoxide solution. Subsequently, 7.282 mL of the activated folic acid solution was added, and the reaction was performed with magnetic stirring for 3 days. After the reaction, the resulting solution was dialyzed against distilled water (6 times, 2 L / time). Then freeze-drying is carried out to obtain folic acid-modified dendrimer-stabilized gold nanoparticles before acetylation, which are stored at -20°C.
[0051] 1 The results of the H NMR spectrum showed that the proton peak of folic acid appeared between 6-9ppm, indicating that the folic acid was successfully modified on the surface of t...
Embodiment 3
[0053] Take 80.30 mg of folic acid-modified dendrimer-stabilized gold nanoparticles before acetylation prepared in Example 2, and dissolve them in 8 mL of distilled water. Add 0.245 mL of triethylamine and stir for 0.5 h. After that, 0.139 mL of acetic anhydride was added, and the reaction was stirred for 24 h. After the reaction, the reaction mixture was dialyzed with PBS buffer (3 times, 2 L / time) and distilled water (3 times, 2 L / time). Then freeze-drying is performed to obtain folic acid-modified dendrimer-stabilized gold nanoparticles, which are stored at -20°C.
[0054] 1 The results of the H NMR spectrum showed that the peak of acetylmethyl group (1.87ppm) appeared after the acetylation reaction, indicating that the acetylation reaction was successfully carried out (attached figure 1 ). The UV-Vis test results show that the absorption peak in the spectrum is located at around 525nm (attached figure 2 ). This indicates that after acetylation, the size of gold nano...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com