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Self-reinforced bi-crosslinking hyaluronic acid hydrogel and preparation method thereof

A hyaluronic acid and hydrogel technology, applied in the field of biomedical materials and tissue engineering, can solve the problems of lack of binding and controlled release of growth factors, non-degradable or poor degradation performance, biocompatibility problems, etc. The effect of regeneration and repair, improving mechanical properties and high water absorption

Active Publication Date: 2013-02-27
浙江天妍生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Inorganic nanoparticles and hydrophobic polymer micro-nanoparticles can effectively strengthen the hyaluronic acid hydrogel, but they cannot degrade or have poor degradation performance in the human body, and there are biocompatibility problems at the same time, and they have certain toxic effects on cells
In addition, the system also lacks the function of binding and controlling the release of growth factors

Method used

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  • Self-reinforced bi-crosslinking hyaluronic acid hydrogel and preparation method thereof
  • Self-reinforced bi-crosslinking hyaluronic acid hydrogel and preparation method thereof
  • Self-reinforced bi-crosslinking hyaluronic acid hydrogel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Add hyaluronic acid into deionized water to prepare a hyaluronic acid aqueous solution with a mass concentration of 0.002g / mL. Under magnetic stirring, add triethylamine (the molar quantity added is 20% of all the hydroxyl moles of hyaluronic acid), add tetrabutylammonium bromide after reacting for 1h (the molar quantity added is all the hydroxyl moles of hyaluronic acid 20% of the number of moles of hyaluronic acid), after reacting for 1 hour, add glycidyl methacrylate to the system (the molar amount added is 50 times the number of moles of carboxyl groups of hyaluronic acid), react at 20°C for 2 days, and keep warm at 60°C 1h, then dialyzed in 0.1mol / L sodium chloride solution and deionized water for 7d, and finally freeze-dried for 16h to obtain double bond-activated hyaluronic acid with a double bond substitution degree of 32.86%. like figure 1 Shown are hyaluronic acid and double bond activated hyaluronic acid 1 H NMR spectrum. like figure 1 As shown in the...

Embodiment 2

[0046] (1) Add hyaluronic acid into deionized water to prepare a hyaluronic acid aqueous solution with a mass concentration of 0.002g / mL. Under magnetic stirring, add triethylamine (the molar quantity added is 20% of all the hydroxyl moles of hyaluronic acid), add tetrabutylammonium bromide after reacting for 1h (the molar quantity added is all the hydroxyl moles of hyaluronic acid 20% of the number of moles of hyaluronic acid), after reacting for 1 hour, add glycidyl methacrylate to the system (the molar amount added is 10 times the number of moles of carboxyl groups of hyaluronic acid), react at 30°C for 2 days, and keep warm at 60°C 1h, then dialyzed in 0.1mol / L sodium chloride solution and deionized water for 7d, and finally freeze-dried for 12h to obtain double bond-activated hyaluronic acid with a double bond substitution degree of 2.8%.

[0047] (2) Dissolve hyaluronic acid in 0.2 mol / L sodium hydroxide solution to prepare a hyaluronic acid aqueous solution with a mass ...

Embodiment 3

[0050] (1) Add hyaluronic acid into deionized water to prepare a hyaluronic acid aqueous solution with a mass concentration of 0.002g / mL. Under magnetic stirring, add triethylamine (the molar quantity added is 20% of all the hydroxyl moles of hyaluronic acid), add tetrabutylammonium bromide after reacting for 1h (the molar quantity added is all the hydroxyl moles of hyaluronic acid 20% of the number of moles of hyaluronic acid), after reacting for 1 hour, add glycidyl methacrylate to the system (the molar amount added is 100 times the number of moles of carboxyl groups of hyaluronic acid), react at 25°C for 2 days, and keep warm at 60°C 1h, then dialyzed in 0.1mol / L sodium chloride solution and deionized water for 7d, and finally freeze-dried for 24h to obtain double bond-activated hyaluronic acid with a double bond substitution degree of 65.0%.

[0051] (2) Dissolve hyaluronic acid in 0.2 mol / L sodium hydroxide solution to prepare a hyaluronic acid aqueous solution with a mas...

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Abstract

The invention, belonging to the technical field of biomedical materials and tissue engineering, discloses a self-reinforced bi-crosslinking hyaluronic acid hydrogel and a preparation method thereof. The preparation method disclosed herein comprises the following steps: preparing double-bond activated hyaluronic acid, preparing hyaluronic acid microballoons, preparing double-bond activated hyaluronic acid microballoons, and preparing the self-reinforced bi-crosslinking hyaluronic acid hydrogel. The self-reinforced bi-crosslinking hyaluronic acid hydrogel prepared by the method is obtained by using double-bond activated hyaluronic acid microballoons as reinforced particles to react with the double-bond activated hyaluronic acid molecules, and has a bi-crosslinking network structure, wherein the diameter of the double-bond activated hyaluronic acid microballoons is 1-10 mum, the pore size of the bi-crosslinking hyaluronic acid hydrogel is 10-70 mum, and the double-bond substitution degree is 2.8-65%. Compared with a single crosslinking hyaluronic acid hydrogel, the hydrogel disclosed herein has better elastic energy, and well prolongs the sustainable control release time of bovine serum albumin.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials and tissue engineering, and in particular relates to a self-reinforced double-crosslinked hyaluronic acid hydrogel and a preparation method thereof. Background technique [0002] Human tissue defects and functional decline due to aging or accidents have seriously affected human health and quality of life. At present, the world spends hundreds of billions of dollars on tissue repair for patients every year, and due to insufficient tissue donors As a result, many patients cannot receive timely treatment. In recent years, the development of tissue engineering has provided an important way for tissue regeneration and functional recovery. Hyaluronic acid hydrogel has excellent properties such as inherent biocompatibility, high water absorption, injectability, and structural similarity to natural extracellular matrix, and is widely used in drug release and tissues such as cartilage, nerves...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08J3/28C08J3/24C08L5/08C08B37/08B01J13/02
Inventor 赵剑豪罗春红屠美曾戎容建华
Owner 浙江天妍生物科技有限公司
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