Preparation method for probiotics milk powder
A technology for probiotics and milk powder, applied in the field of food processing, can solve the problems of changing the original flavor of probiotics, inconvenient transportation and storage, etc., and achieve the effects of low equipment requirements, simple operation and high bacterial activity.
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[0029] Example 1
[0030] The preparation method of probiotic milk powder includes the following steps:
[0031] (1) Raw milk processing: the raw milk (cow milk) is sterilized at 135°C for 1 to 2 seconds at ultra-high temperature; then, it is concentrated under reduced pressure in a vacuum concentration tank, and the vacuum degree is maintained at 15.67×10 3 Pa, the temperature is maintained at 51°C, and concentrated milk is obtained after concentration.
[0032] (2) Bacterial cultivation: 3ml of probiotics (Streptococcus thermophilus) were inoculated into 300ml of fresh milk sterilized at 115°C for 10 minutes, and incubated at 37°C until curd.
[0033] (3) Centrifuge the obtained curd at 4°C and 6000r / min for 10 minutes to collect probiotic bacteria;
[0034] The obtained probiotic bacteria culture was added to concentrated milk in an amount of 4% by mass, and a protective agent was added to include leucine and vitamin B 12 The mass concentrations of glutamic acid and glutamic acid are...
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[0037] Example 2
[0038] The preparation method of probiotic milk powder includes the following steps:
[0039] (1) Treatment of raw milk: the raw milk is subjected to ultra-high temperature instantaneous sterilization at 120°C for 1 to 2 seconds; then, it is immediately concentrated under reduced pressure in a vacuum concentration tank, and the vacuum degree is maintained at 16.00×10 3 Pa, the temperature is maintained at 53°C, and concentrated milk is obtained after concentration, with a milk solid content of 40-50%.
[0040] (2) Bacterial cell culture: first prepare an enrichment medium, dissolve 10g of whey powder, 5g of yeast extract, 25g of corn starch, and 180g of cucumber extract in 1000ml of distilled water, mix well, and sterilize at 115℃ Bacteria 20min;
[0041] Inoculate 10ml of probiotic strains (Streptococcus thermophilus and Lactobacillus bulgaricus, mixed in a volume ratio of 1:1) in the above-mentioned enrichment medium, and culture at 38°C for 32 hours. The somatic...
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[0045] Example 3
[0046] The preparation method of probiotic milk powder includes the following steps:
[0047] (1) Treatment of raw milk: the raw milk is sterilized at 150°C for 1 to 2 seconds at ultra-high temperature; then, it is immediately concentrated under reduced pressure in a vacuum concentration tank, and the vacuum degree is maintained at 15.45×10 3 Pa, the temperature is maintained at 55°C, and concentrated milk is obtained after concentration, with a milk solid content of 40-50%.
[0048] (2) Bacterial cell culture: first prepare an enrichment medium, dissolve 10g of whey powder, 5g of yeast extract, 25g of corn starch, 100g of a mixture of white radish extract and carrot extract in 1000ml of distilled water, and mix well. , Sterilize at 115℃ for 20min;
[0049] Inoculate 10ml of probiotic strains (Lactobacillus bulgaricus) in the above-mentioned enrichment medium, culture at 37°C for 48h to obtain the bacterial culture solution, centrifuge the bacterial culture at 4°C, ...
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