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Wedelia prostrate polysaccharide as well as preparation method and application of wedelia prostrate polysaccharide

A technology of polysaccharide and chrysanthemum halothi, applied in application, tobacco, food science and other directions, can solve the problems of high cost, low yield, low purity, etc., achieve simple operation, high impurity removal rate, and ensure accuracy Effect

Active Publication Date: 2013-03-13
CHINA TOBACCO ZHEJIANG IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent application CN201110236229.1 discloses a method for extracting American ginseng polysaccharides, which is carried out according to the following steps: 1. Soak American ginseng in water, decoct, and filter while it is hot; 2. Decoct twice with water, combine the water Concentrate the decoction, place it for cooling; 3. Precipitate with alcohol, filter, volatilize the filter residue until it has no alcohol taste, dry it at 60-90°C, and pulverize it to obtain the American ginseng polysaccharide; this method uses water to extract the American ginseng polysaccharide, which reduces the cost. The extraction of American ginseng polysaccharides has high purity and high yield. The polysaccharide purity is above 38.8%, and the polysaccharide yield is above 4.7%. , technical problems of low yield
In view of the complexity of the polysaccharide structure, there are few studies on the specific structure of polysaccharides

Method used

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  • Wedelia prostrate polysaccharide as well as preparation method and application of wedelia prostrate polysaccharide
  • Wedelia prostrate polysaccharide as well as preparation method and application of wedelia prostrate polysaccharide
  • Wedelia prostrate polysaccharide as well as preparation method and application of wedelia prostrate polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Crush the dried whole-plant sample of Chrysanthemum chrysanthemum to 40 mesh, add a certain amount of distilled water according to the ratio of solid to liquid (mass ratio) 1:20, extract with boiling water at 100°C for 2 times, each time for 3 hours, and combine the 2 extracts to obtain the total Extraction solution;

[0043] After the total extract was cooled, it was centrifuged at a high speed (10,000 rpm) at 25°C for 15 minutes and vacuum filtered, then concentrated in vacuum to 1 / 10 of the volume of the original total extract, and an aqueous ethanol solution with a volume fraction of 95% was added to the concentrate to make The volume fraction of ethanol in the system reaches 50%, stir, stand still for 10 hours, centrifuge, collect the precipitate, freeze-dry to obtain the crude polysaccharide;

[0044] Dissolve the crude polysaccharide in water, put it on a DEAE-Sepharose Fast Flow ion column (column type is XK 26×100cm diethylaminoethyl sepharose ion exchange colu...

Embodiment 2

[0048] Crush the dried sample of the whole plant of Chrysanthemum chrysanthemum to 40 mesh, add a certain amount of distilled water according to the ratio of solid to liquid (mass ratio) 1:30, extract with boiling water at 100°C for 2 times, each time for 3 hours, and combine the 2 extracts to obtain the total Extraction solution;

[0049] After the total extract was cooled, it was centrifuged at a high speed (10,000 rpm) at 25°C for 15 minutes and vacuum filtered, then concentrated in vacuum to 1 / 20 of the volume of the original total extract, and an aqueous ethanol solution with a volume fraction of 95% was added to the concentrate to make The volume fraction of ethanol in the system reaches 60%, stir, stand still for 10 hours, centrifuge, collect the precipitate, freeze-dry to obtain the crude polysaccharide;

[0050] Dissolve the crude polysaccharide in water, put it on a DEAE-Sepharose Fast Flow ion column (column type is XK 26×100cm) ion exchange column, elute with disti...

Embodiment 3

[0056] Grind the dried whole plant of Chrysanthemum chrysanthemum to 80 mesh, add a certain amount of distilled water according to the ratio of solid to liquid (mass ratio) 1:40, extract with boiling water at 100°C for 2 times, each time for 3 hours, and combine the 2 extracts to obtain the total Extraction solution;

[0057] After the total extract was cooled, it was centrifuged at a high speed (10,000 rpm) at 25°C for 15 minutes and vacuum filtered, then concentrated in vacuum to 1 / 10 of the volume of the original total extract, and an aqueous ethanol solution with a volume fraction of 95% was added to the concentrate to make The volume fraction of ethanol in the system reaches 65%, stir, stand still for 10 hours, centrifuge, collect the precipitate, freeze-dry to obtain the crude polysaccharide;

[0058] Dissolve the crude polysaccharide in water, put it on a DEAE-Sepharose Fast Flow ion column (column type is XK 26×100cm) ion exchange column, elute with distilled water, co...

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Abstract

The invention discloses a wedelia prostrate polysaccharide as well as a preparation method and an application of the wedelia prostrate polysaccharide. The main structure of the wedelia prostrate polysaccharide is as follows: beta-D-fructan in 2-6 connection is taken as a main chain, and alpha-D-glucose connected with the main chain 1-2 is taken as an end group. The wedelia prostrate polysaccharide has good moisturizing effect and can be used as a moisturizer for cigarette tobacco. The invention also discloses a preparation method of the wedelia prostrate polysaccharide. The preparation method comprises the following extraction steps of sample drying, grinding, extraction, centrifugation, filtration, concentration, alcohol precipitation, and freeze-drying to obtain crude polysaccharide. The preparation method comprises the following separation and purification steps of: carrying out ion exchange column chromatography on the crude polysaccharide, collecting, carrying out freeze-drying, carrying out gel column chromatography, collecting, and carrying out freeze-drying to obtain pure polysaccharide. The preparation method is simple to operate, pollution-free, good in separation and purification effects, and high in impurity removal rate.

Description

technical field [0001] The invention relates to the field of plant polysaccharides, in particular to a chrysanthemum polysaccharide and its preparation method and application. Background technique [0002] Wedelia prostrata (Hook.Et Arn.) Hemsl. is a plant of the genus Wedelia in Asteraceae, which is widely distributed in Zhejiang. At present, the research is mainly on the diterpenoids, sesquiterpenes, flavonoids, and organic acids of chrysanthemum, but the research and application reports on its polysaccharides have not yet appeared. [0003] Polysaccharides are high molecular polymers composed of more than 10 monosaccharides linked by glycosidic bonds. The separation and purification of carbohydrate compounds is one of the difficulties in carbohydrate research. Different extraction and purification methods will not only directly affect the extracted polysaccharides The different properties of polysaccharides may also lead to changes in the active structure. Therefore, it ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A24B15/40
Inventor 杨君储国海黄芳芳蒋健张安强
Owner CHINA TOBACCO ZHEJIANG IND
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