Microwave-assisted xylo-oligosaccharide preparation method

A xylo-oligosaccharide and microwave-assisted technology, which is applied in the field of microwave-assisted preparation of xylo-oligosaccharide, can solve the problems of difficult separation and purification, high production cost, and long time consumption, and achieve easy-to-obtain raw materials, large amounts of raw materials, and high product value high effect

Active Publication Date: 2013-03-13
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the above xylo-oligosaccharide preparation process and method, the method of directly degrading biomass has defects such as low efficiency, the obtained product contains more impurities, difficult separation and purification, and high production cost.
The enzymatic method is time-consuming, and xylan substrates from different sources require the use of different microbial xylanases.

Method used

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  • Microwave-assisted xylo-oligosaccharide preparation method
  • Microwave-assisted xylo-oligosaccharide preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A kind of microwave-assisted method for preparing xylo-oligosaccharides of the present embodiment comprises the following steps:

[0037] a. Extraction of xylan:

[0038] (a1) Weigh 100 g of 40-60 mesh bamboo powder, and reflux with 500 ml of toluene-ethanol (2:1, v / v) for 12 hours to remove lipids in the raw material;

[0039] (a2) Remove lignin from the degreased bamboo powder with 2000ml of 7.5% sodium chlorite solution at 75°C and pH 3.8 for 2 hours to prepare bamboo cellulose;

[0040] (a3) Extract cellulose with 2000ml 10% NaOH at room temperature for 12 hours, filter, adjust the pH of the filtrate to 5.5 with 6M HCl, and dialyze in a dialysis bag with a molecular weight cut-off of 3500Da for 3 days;

[0041] (a4) Freeze-drying to obtain xylan, the temperature is -40°C, the vacuum degree is 200mba, and the time is 24h;

[0042] b. Preparation of xylo-oligosaccharides: the xylo-oligosaccharides obtained in step a are added to 0.2M sulfuric acid, the solid-liquid ...

Embodiment 2

[0047] a. Extraction of xylan:

[0048] (a1) Weigh 100g of Populus tomentosa powder of 50-80 mesh, and reflux with 500ml of toluene-ethanol (2:1, v / v) for 6h to remove lipids in the raw material;

[0049] (a2) Degreasing poplar tomentosa powder with 2000ml of sodium chlorite solution with a mass fraction of 7.5% at 75°C and pH 3.8 for 2 hours to prepare poplar tomentosa cellulose;

[0050](a3) Extract cellulose with 2500ml 10% KOH at room temperature for 8 hours, filter, adjust the pH of the filtrate to 5.5 with acetic acid, and dialyze in a dialysis bag with a molecular weight cut-off of 3800Da for 3 days;

[0051] (a4) To obtain xylan by freeze-drying, the temperature is -50°C, the vacuum degree is 400mba, and the time is 30h;

[0052] b. Preparation of xylo-oligosaccharides: Add the xylo-oligosaccharides obtained in step a into 0.2M oxalic acid, the ratio of solid to liquid (g / ml) is 1:30, degrade at a temperature of 80°C for 40 minutes, and use a power of 800W at the same...

Embodiment 3

[0057] a. Extraction of xylan:

[0058] (a1) Weigh 100g of 40-60 mesh wheat straw powder, and reflux with 500ml of toluene-ethanol (2:1, v / v) for 9 hours to remove lipids in the raw material;

[0059] (a2) Remove lignin from defatted wheat straw powder with 2000ml of 6% sodium chlorite solution at 77°C and pH 3.6 for 2 hours to prepare wheat straw cellulose;

[0060] (a3) Use 2300ml of 8% KOH to extract the cellulose at room temperature for 10 hours, filter, adjust the pH of the filtrate to 5.5 with acetic acid, and then dialyze in a dialysis bag with a molecular weight cut-off of 2000Da for 2 days;

[0061] (a4) Xylan was obtained by vacuum drying at a temperature of 30°C and a vacuum of 0.8 MPa for 48 hours;

[0062] b. Preparation of xylo-oligosaccharides: Add the xylo-oligosaccharides obtained in step a into 0.1M formic acid, the ratio of solid to liquid (g / ml) is 1:25, degrade at a temperature of 90°C for 60 minutes, and use a power of 800W at the same time microwave tr...

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Abstract

The invention discloses a microwave-assisted xylooligosaccharide preparation method. The method comprises the steps of: a. extracting polyxylose from wood fiber raw material with an alkaline process; b. adding the polyxylose obtained from the step a into 0.1-0.4M acid, degrading for 10-90min at the temperature condition of 70-100 DEG C and treating by using microwave with power of 500-800W at the same time, wherein the massic volume (g/ml) of the polyxylose to the acid is 1:20-100; c. centrifuging or filtering for separating the degradation liquid obtainedin the step b, and neutralizing the supernate or filtrate; and d. carrying out freeze drying or vacuum drying on the neutralized supernate or filtrate to obtain xylooligosaccharide. The method is fast, high-efficiency, generates few side effects, and the purity of the prepared xylooligosaccharide is high.

Description

technical field [0001] The invention relates to the technical field of xylooligosaccharide preparation, in particular to a method for microwave-assisted preparation of xylooligosaccharide. Background technique [0002] Xylo-oligosaccharides (Xylo-oligosaccharides, XOS), also known as xylooligosaccharides, are composed of 2-7 xylose molecules linked by β-1,4 glycosidic bonds and have a linear or side chain structure. Polysaccharides, with a molecular weight of about 300-2000 Da, are generally formed by degradation of xylan. Xylo-oligosaccharides have unique physicochemical properties and physiological properties. In terms of physical and chemical properties, XOS has heat and acid resistance, low viscosity, can reduce water activity and prevent freezing. In terms of physiological properties, XOS is non-digestible but can be fermented Sugar is almost not degraded by the digestive juice in the human body, and cannot be directly used and absorbed by the human body, but it can be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C13K13/00
Inventor 边静肖霄孙润仓彭霄鹏彭锋
Owner BEIJING FORESTRY UNIVERSITY
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