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Poultry transfer factor nanoparticle and preparation method thereof

A technology of transfer factor and nanoparticles, which is applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, peptide/protein components, etc., can solve the problems of inability to fully exert the role of transfer factor, insufficient research and application of transfer factor, and low bioavailability, etc. problem, achieve the effect of improving poultry stability, improving bioavailability, and simple preparation process

Active Publication Date: 2013-03-20
河南牧翔生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But research on transfer factor is underutilized in veterinary medicine
Since transfer factor is a small peptide substance, it distributes quickly in the body, has a short action time, low bioavailability, and transfer factor can be degraded by hydrolytic enzymes, so it cannot fully play the role of transfer factor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Accurately weigh 0.7g of polyvinyl alcohol and 0.7g of poloxamer 188, dissolve them completely with 97.9g of distilled water, add 0.1g of poultry transfer factor, adjust the pH to 3, add 0.6g of n-butyl cyanoacrylate dropwise under magnetic stirring , continue stirring for 6 hours and then adjust the pH value to 7 to obtain a colloidal solution of light yellow poultry transfer factor n-butyl cyanoacrylate nanoparticles. Finally, the obtained solution is filtered through a 0.45um microporous membrane and then freeze-dried at -40°C , to obtain the freeze-dried powder of the present invention. The transfer factor freeze-dried powder of the present invention is analyzed and detected by a transmission electron microscope and a particle size analyzer, and the average particle diameter is 140nm, the particle size is uniform, the shape is round, and there is no adhesion.

Embodiment 2

[0023] Accurately weigh 2 g of polyvinyl alcohol and 1 g of poloxamer 188, dissolve them completely with 94.7 g of distilled water, add 0.3 g of poultry transfer factor, adjust the pH to 4, add 2 g of n-butyl cyanoacrylate drop by drop under magnetic stirring, and continue stirring After 12 hours, the pH value was adjusted to 10 to obtain a colloidal solution of light yellow poultry transfer factor n-butyl cyanoacrylate nanoparticles. Finally, the obtained solution was filtered through a 0.45um microporous membrane and then freeze-dried at -70°C to obtain Freeze-dried powder of the present invention. The transfer factor freeze-dried powder of the present invention is analyzed and detected by a transmission electron microscope and a particle size analyzer, and the average particle diameter is 129nm, the particle size is uniform, the shape is round, and there is no adhesion.

Embodiment 3

[0025] Accurately weigh 0.5 g of polyvinyl alcohol and 0.7 g of poloxamer 188, dissolve them completely with 98.2 g of distilled water, add 0.25 g of poultry transfer factor, adjust the pH to 3, add 0.9 g of n-butyl cyanoacrylate drop by drop under magnetic stirring , continue to stir for 5 hours and adjust the pH value to 6 to obtain a colloidal solution of light yellow poultry transfer factor n-butyl cyanoacrylate nanoparticles. Finally, the obtained solution is filtered through a 0.45um microporous membrane and then freeze-dried at -60°C , to obtain the freeze-dried powder of the present invention. The transfer factor freeze-dried powder of the present invention is analyzed and detected by a transmission electron microscope and a particle size analyzer, and the average particle diameter is 140nm, the particle size is uniform, the shape is round, and there is no adhesion.

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Abstract

The present invention belongs to the field of veterinary medicines, and specifically discloses poultry transfer factor nanoparticles and a preparation method thereof. The nanoparticles are made from the following raw materials by weight: 0.1-0.3% of poultry transfer factor, 0.6-2.6% of a carrier, 0.5-3% of a stabilizer, 0.6-1% of a surfactant, and 94-99.4% of water. After the stabilizer and the surfactant are completely dissolved in water, the poultry transfer factor is added, the pH value is adjusted to 3-4, n-butylcyanoacrylate is added under magnetic stirring, the stirring continues for 5-12h, the pH value is adjusted to 6-10, and a pale yellow transfer factor nanoparticle colloidal solution is obtained. The prepared solution is filtered with membrane and subsequently freeze-dried to prepare a freeze-drying powder under the condition of a temperature from -40 to -70 DEG C. The poultry transfer factor encapsulated by nanoparticles can prevent being degraded by pepsin, is conductive to intestinal absorption, can improve the stability of the poultry transfer factor, deliver drug targetedly, release drugs slowly, extend the time of drug action, and improve the bioavailability of the poultry transfer factor, and solves the shortcoming of low efficacy of oral transfer factor.

Description

technical field [0001] The invention belongs to the field of veterinary medicine, and relates to a new dosage form of avian transfer factor, in particular to a nanoparticle of avian transfer factor and a preparation method thereof. Background technique [0002] The addition of antibiotics to poultry feed and the high-volume use of drugs in disease prevention and control have resulted in drug residues in food animals, severely restricting the export of meat products, and harming human life and health. At present, food safety issues have become the focus of public health in the world today. [0003] Transfer factor has the functions of transmitting immune information, stimulating immune cell activity, regulating immune function, enhancing the body's phenanthrene-specific cellular immune function, etc., and because of its small molecular weight, non-toxicity, no species barrier, no antigenicity, and no allergic reaction It is an ideal biological immune enhancer and regulator i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/19A61K35/28A61K38/00A61P37/02A61P37/04A61K35/57
Inventor 胡帅梁军樊楠李坤鹏
Owner 河南牧翔生物科技有限公司
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