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Chemiluminescence immunoassay system, as well as method and application thereof

A chemiluminescence immunoassay and analysis system technology, applied in the field of medical equipment and biological detection, can solve the problems of limited application flexibility and high price

Active Publication Date: 2014-09-10
GUANGDONG HEXIN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the chemiluminescent immunoassay instruments used in laboratory medicine are large-scale equipment. Although the test throughput is high, they are expensive and have limited application flexibility.

Method used

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  • Chemiluminescence immunoassay system, as well as method and application thereof
  • Chemiluminescence immunoassay system, as well as method and application thereof
  • Chemiluminescence immunoassay system, as well as method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Such as figure 1 As shown, a chemiluminescent immunoassay system includes a three-dimensional motion sampling platform, a temperature control module, a magnetic field control module, a fluid control unit and an optical detection module; a three-dimensional motion sampling platform, a temperature control module, a magnetic field control module and an optical detection module The modules are connected in sequence, and the fluid control unit is connected with the temperature control module;

[0064] The three-dimensional motion sampling platform is composed of a sample / reagent carrying tray 2, a displacement stage 1b, a lead screw and a drive motor 1a; the sample / reagent carrying tray 2 is arranged on the top of the displacement stage 1b, and the displacement stage 1b is controlled by the drive motor 1a down movement;

[0065] The temperature control module is provided with a PT-100 temperature sensor 8, a temperature controller 16, a relay 17, a cover plate 7 sequential...

Embodiment 2

[0074] The analysis method carried out using the chemiluminescence immunoassay system of embodiment 1 may further comprise the steps:

[0075] (1) Introduce 5 μL of mineral oil, 2 μL of magnetic bead suspension, and 5 μL of the serum sample to be tested into 12 parallel polytetrafluoroethylene capillaries in sequence; the length of the capillary used is 10 cm, and the inner diameter is 1000 μm; the surface of the magnetic beads used is immobilized with anti-tumor necrosis factor Beta antibody (anti-TNF Beta, mouse anti-human), magnetic beads dispersed in phosphate buffer solution, the mass volume percentage is 0.25g / mL; the control liquid section migrates to the temperature control area at a speed of 1cm / s, and is kept at 37°C for 2 minute;

[0076] (2) When the droplet moves to the magnetic field area, start the electromagnet above the capillary (magnetic field strength 150mT), and fix the magnetic beads on the capillary wall;

[0077](3) Control the micro-syringe to dischar...

Embodiment 3

[0084] The analysis method carried out using the chemiluminescence immunoassay system of embodiment 1 may further comprise the steps:

[0085] (1) Introduce 5 μL of mineral oil, 2 μL of magnetic bead suspension and 5 μL of the serum sample to be tested into two parallel glass capillaries in sequence; the length of the capillary used is 50 cm, and the inner diameter is 500 microns; Bead particles are dispersed in phosphate buffer solution with a mass volume percentage of 0.1g / mL; the control liquid section migrates to the temperature control zone at a speed of 1cm / s, and is kept at 20°C for 10 minutes;

[0086] (2) When the droplet moves to the magnetic field area, start the electromagnet above the capillary (magnetic field strength 150mT), and fix the magnetic beads on the capillary wall;

[0087] (3) Control the micro-syringe to discharge the waste liquid; control the micro-syringe to introduce the washing solution into the capillary (the composition is 0.1mol / L Na 2 HPO 4 ...

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Abstract

The invention discloses a chemiluminescence immunoassay system, as well as a method and an application thereof. The chemiluminescence immunoassay system comprises a fluid control unit as well as a three-dimensional motion sample feeding platform, a temperature control unit, a magnetic field control module and an optical detecting module, wherein the three-dimensional motion sample feeding platform, the temperature control unit, the magnetic field control module and the optical detecting module are connected with each other in sequence; and the fluid control unit is connected with the temperature control unit. The chemiluminescence immunoassay system disclosed by the invention has the advantages as follows: with a capillary tube as a reactor, reagent transportation and reaction are both carried out in a water-in-oil type liquid drop by using a capillary tube liquid drop technology, so that a stable reaction condition is kept, and crossed pollution between samples and reaction suppression caused by surface adsorption are effectively avoided. The chemiluminescence immunoassay system disclosed by the invention has the advantages of small size, high assay speed, high testing flux, automatic operation and flexibility in application and is suitable for analyzing single sample, batch samples and field rapid assay; medical demands of the masses can be preferably satisfied; meanwhile, the purchasing and operation cost of equipment is obviously reduced, and excellent social and economic benefits are obtained.

Description

technical field [0001] The invention belongs to the technical field of medical equipment and biological detection, and in particular relates to a chemiluminescence immunoassay system and its analysis method and application. Background technique [0002] Chemiluminescence immunoassay (CLIA) combines high-sensitivity chemiluminescence assay technology with high-specificity immune response for various antigens, haptens, antibodies, hormones, enzymes, fatty acids, vitamins and drugs, etc. detection and analysis technology. CLIA has the advantages of high sensitivity, strong specificity, low reagent price, stable reagents and valid period (6-18 months), stable and fast method, wide detection range, simple operation and high degree of automation. The fields of science, environment and material science have been promoted, especially in the field of laboratory medicine, which has formed a huge market for chemiluminescence immunoassay. [0003] Arakawe first reported CLIA in the mi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/538G01N21/76
Inventor 刘大渔
Owner GUANGDONG HEXIN BIOTECH
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