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Compound encapsulated lactic acid bacteria product and preparation method thereof

A technology for lactic acid bacteria and lactic acid bacteria fermentation liquid, applied in the field of lactic acid bacteria microcapsule products and their preparation, can solve problems such as poor temperature resistance and stability of lactic acid bacteria, and achieve the effects of avoiding bacterial activity loss, reducing production power costs, and improving tolerance

Active Publication Date: 2014-04-23
BEIJING HESWOF BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is: 1. solve the problem that the lactic acid bacterium prepared by the prior art has poor temperature resistance stability, the lactic acid bacterium prepared by the present invention is 85 ℃ heat-resistant 3 minutes, and survival rate is more than 90%

Method used

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  • Compound encapsulated lactic acid bacteria product and preparation method thereof
  • Compound encapsulated lactic acid bacteria product and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The preparation of embodiment 1 coated lactic acid bacteria

[0035] (1) Preparation of lactic acid bacteria fermentation broth

[0036] ①Preparation of primary seed liquid: take 1 bacterial cell preserved on a slant plane, inoculate it into the primary seed liquid medium, cultivate it in a shaker at 37±0.5°C and 180rpm / min for 8±1h after inoculation, and obtain a Grade Shake Flask Seed Solution.

[0037] The proportioning (weight-to-volume ratio W / V) method of the medium of the primary seed is as follows: glucose 1-2%, soybean peptone 1-3%, yeast extract 0.5-1%, magnesium chloride 0.05-0.1%, sodium chloride 0.2% -1%, calcium carbonate 0.2-0.5%, the filling amount of first-grade seeds is 40%, sterilized at 121°C for 30min, and ready for use.

[0038] ②Preparation of the secondary fermentation liquid: transfer the primary seed liquid to the secondary seed medium, the inoculation amount is 1-5%, the tank temperature after inoculation is controlled at 38±0.5°C, and the r...

Embodiment 2

[0059] Embodiment 2 coated lactic acid bacteria gastric acid resistance assay

[0060] The impact of gastric acid on the survival rate of microencapsulated coated lactic acid bacteria lactic acid bacteria is shown in Table 1. The residence time of food in the stomach of livestock and poultry animals is generally 1-2 hours. This experiment artificially simulates the gastric juice environment. When the pH value of the gastric juice is 4, after 120 minutes, the survival rate of lactic acid bacteria is 95%. 3. After 120 minutes, the survival rate of lactic acid bacteria is 86%. When the pH value of gastric juice is 2, after 120 minutes, the survival rate of lactic acid bacteria is still 83%.

[0061] Table 1 Effect of gastric acid on the survival rate of microencapsulated coated lactic acid bacteria

[0062]

[0063] Tests show that the microencapsulated coated lactic acid bacteria prepared by the invention has better gastric acid resistance, can pass through the stomach of an...

Embodiment 3

[0064] Embodiment 3 coating lactic acid bacteria bile-resistant determination

[0065] The effect of bile salts on the survival rate of microencapsulated coated lactic acid bacteria is shown in Table 2. As seen from Table 2, along with the raising of bile salt concentration, the prolongation of time, the survival rate of lactic acid bacteria constantly reduces, when bile salt concentration 0.3%, the survival rate of lactic acid bacteria after 3 hours is 89%, when bile salt concentration 0.5% , the survival rate of lactic acid bacteria was still 85% after 3 hours.

[0066] Table 2 The impact of bile salts on the survival rate of microencapsulated lactic acid bacteria

[0067]

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Abstract

The invention relates to a compound encapsulated lactic acid bacteria microcapsule product and a preparation method thereof. According to the encapsulated lactic acid bacteria microcapsule product, the temperature-resistant stability of lactic acid bacteria is improved. The preparation method of the encapsulated lactic acid bacteria is finished by a two-stage compound encapsulation process consisting of encapsulation before fermentation and encapsulation after fermentation, wherein the encapsulation before fermentation refers to the step of laying an encapsulation material on the surface layer of bacteria cells to form a film layer, so that the tolerance of the lactic acid bacteria is improved in the processing process; and the encapsulation after fermentation comprises the following steps of: centrifugally separating lactic acid bacteria solution after the fermentation is finished, adding a stable protective agent to the lactic acid bacteria solution, pelletizing the solution for forming, and encapsulating a material on pellets after spraying, thus forming film layers on the surface layers of lactic acid bacteria microcapsules, wherein the encapsulated material is an enteric-soluble natural high polymer material. The lactic acid bacteria provided by the invention is excellent in temperature-resistant stability and has high enteric-soluble slow release properties.

Description

technical field [0001] The invention relates to a compound-coated lactic acid bacterium, which belongs to the technical field of microbial feed additives, and relates to a lactic acid bacteria microcapsule product composed of a compound coating material, a bacteria protectant and lactic acid bacteria and a preparation method thereof. Background technique [0002] Due to the long-term, excessive and irrational use or abuse of antibiotics in livestock and poultry farming and feed, a series of problems have emerged, such as causing animal flora imbalance, disorder or ectopic transfer of conditional pathogenic bacteria, multi-resistant The production of drug-resistant strains, residues in livestock and poultry products (such as meat, eggs, milk) and environmental pollution, especially antibiotic-associated diarrhea and drug-resistant diarrhea, are seriously endangering the healthy development of animal husbandry, leading to Ineffective or increased dosage, a vicious circle. At ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/00
Inventor 李雪平
Owner BEIJING HESWOF BIOTECH CO LTD
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