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Treatment method for flocculate of heparin sodium enzymatic hydrolysate

A technology of heparin sodium enzymolysis solution and treatment method, which is applied in separation methods, chemical instruments and methods, precipitation separation, etc., can solve problems such as resin poisoning and poor adsorption effect, and achieve the effect of reducing difficulty

Inactive Publication Date: 2013-03-27
RUGAO BAXIN CASING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the enzymolysis solution contains more impurities, such as protein, nucleic acid, mycelium, pigment, etc., which will affect the subsequent ion exchange process and may cause poisoning of the resin, resulting in poor adsorption effect.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Add 3-5wt% chitosan to the heparin sodium enzymatic hydrolysis solution, stir the mixed solution at a speed of 100-150r / min for 15-30min, leave it at room temperature for 5-6h, and send it into a centrifuge for centrifugal filtration at a centrifugal speed of 2000-3000r / min, the time is 10min, and the supernatant containing heparin sodium is obtained after removing solid impurities.

[0014] Detection and analysis: the recoveries of proteins, pigments, nucleic acids and mycelia in this example were 51.3%, 14.5%, 53.1% and 83.9%, respectively.

Embodiment 2

[0016] Same as Example 1, the difference is that the above-mentioned chitosan is replaced by deacetylated chitosan derivatives.

[0017] Detection and analysis: the recoveries of proteins, pigments, nucleic acids and mycelia in this example were 62.5%, 10.2%, 58.2% and 87.3%, respectively.

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PUM

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Abstract

The invention discloses a treatment method for flocculate of heparin sodium enzymatic hydrolysate. The treatment method is characterized by comprising the following steps: preparing the heparin sodium enzymatic hydrolysate by adopting a conventional method, adding 3-5wt% of flocculant into the heparin sodium enzymatic hydrolysate, agitating the mixed liquor for 15-30min at the speed of 100-150r / min, standing for 5-6h at room temperature, sending into a centrifugal machine for centrifugal filtering for 10min at the centrifugal speed of 2000-3000r / min, and removing solid impurities to obtain heparin sodium-containing supernatant. The treatment method has the advantages that macromolecular matters of protein, nucleic acid, mycelium and the like in the enzymatic hydrolysate can be flocculated and precipitated by conducting flocculation processing to the heparin sodium enzymatic hydrolysate, thereby laying a foundation for the following separation process, and reducing the following impurity removal difficulty.

Description

technical field [0001] The invention relates to a flocculation treatment method of heparin sodium enzymatic hydrolysis solution. Background technique [0002] Heparin sodium is a mucopolysaccharide sulfate anticoagulant drug, mainly used in the treatment of acute myocardial infarction, can relieve unstable angina, antithrombotic and blood fat, lowering cholesterol. Heparin was first discovered in the liver in 1916, and then found to be more abundant in the small intestinal mucosa of pigs. Therefore, people began to extract heparin sodium from pig small intestinal mucosa, using pig small intestinal mucosa as raw material, including raw material processing, enzymatic extraction, resin adsorption, elution, alcohol precipitation and drying to obtain crude heparin sodium, and then through refining process to obtain heparin Sodium boutique. [0003] In the above process, the extraction effect of the crude heparin sodium directly affects the yield and quality of the refined produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10B01D21/01
Inventor 顾永军
Owner RUGAO BAXIN CASING
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