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Dog source bacillus licheniformis Y10 and application thereof

The technology of Bacillus licheniformis and strain is applied in the field of preventive veterinary medicine, which can solve problems such as inability to treat, difficult to cure, and difficult to cure diseases, and achieves good economic benefits, good bacteriostatic effect, and the effect of restoring intestinal function.

Inactive Publication Date: 2013-03-27
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the current situation that the abuse and prohibition of antibiotics lead to the situation that the disease is difficult to cure and the problem that the dog is difficult to cure or cannot treat the diarrhea caused by bacterial infection, the purpose of the present invention is to provide a strain with acid resistance, bile salt resistance and good bacteriostatic effect. Bacillus licheniformis Y 10 ; also provide the Bacillus licheniformis Y 10 The application in veterinary medicine or feed can be used to prevent and treat canine diarrhea, adjust the microecological balance of canine intestines, and improve the diversity of intestinal microorganisms

Method used

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  • Dog source bacillus licheniformis Y10 and application thereof
  • Dog source bacillus licheniformis Y10 and application thereof
  • Dog source bacillus licheniformis Y10 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: The acquisition and identification of "Bacillus licheniformis CCTCC 2011134" of the present invention, see figure 1

[0026] 1. "Bacillus licheniformis CCTCC 2011134" of the present invention (i.e. Bacillus licheniformis Y 10 ) separation, acquisition

[0027] 1.1 Strain Y 10 separation

[0028] Fresh fecal samples were collected from common, domestically healthy pet dogs located in Ya'an, Sichuan Province, China, and sent immediately to the laboratory for testing. Take 0.5 g of fresh fecal samples, put them into a conical flask with 49.5 ml of normal saline and glass beads, shake on a shaker at 37°C for 20 minutes, and then bathe in a constant temperature water bath at 60°C for 20 minutes. Take 0.5ml of diluted feces and make a ten-fold decremental dilution to 10 -5 . from 10 -3 ~10 -5 100 μL of each dilution was spread on ordinary nutrient agar medium, and incubated at a constant temperature of 37°C for 12h.

[0029] Microscopically examine individua...

Embodiment 2

[0051] Embodiment 2: " probiotic bacillus CCTCC 2011134 " of the present invention (being bacillus Y 10 )Applications

[0052] 1. Bacillus licheniformis Y of the present invention 10 Safety experiment

[0053] (1) Experimental animals: about 40 Kunming mice (half male and half female) weighing about 15 g were purchased from the Animal Experiment Center of West China Medical College, Sichuan University.

[0054] (2) Culture medium: nutrient broth medium (peptone 10g, beef extract 5g, sodium chloride 5g, 1000mL water, pH7.2, sterilized at 120°C for 30min)

[0055] (3) Preparation of bacterial agent: use nutrient agar plate, adopt solid plate culture method, collect bacterial cells, add appropriate amount of calcium carbonate carrier, dry bacterial agent at 60°C, and detect the number of viable bacteria in bacterial agent by dilution method.

[0056] (4) The making of mouse feed: according to the number of living bacteria of the bacterial agent, mix with a certain amount of m...

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Abstract

The invention discloses acid-proof cholate-resisting dog source bacillus licheniformis Y10 with acid resistance, cholate resistance and good antibacterial effect and application of the dog source bacillus licheniformis Y10 in prevention and treatment of dog enteritis and dysentery. The dog source bacillus licheniformis Y10 is obtained through separation, screening and identification, and the preservation number is CCTCC NO: M2011134; the bacillus licheniformis Y10 can be prepared into a micro-ecological agent to be used as a veterinary medicine and a dog food additive for regulating alteration of intestinal flora, increasing microbial diversity and immune organ index of dog intestinal tract, improving dog growth performance, effectively preventing dog dysentery, and providing a new concept for prevention of bacterial diseases of dog intestinal tract and a feasible alternate strain for development of the dog source micro-ecological agent. The dog source bacillus licheniformis Y10 is suitable for feed mills and large-scale dog-breeding kennels.

Description

technical field [0001] The invention relates to an isolated strain of Bacillus licheniformi (Bacillus licheniformi) Y which is acid-resistant, bile salt-resistant, has good antibacterial effect, can significantly improve the intestinal environment of dogs, and promotes micro-ecological balance so as to reduce diarrhea rate. 10 The invention and its application in preventing and treating dog diarrhea belong to the technical field of preventive veterinary medicine. Background technique [0002] Since the 1980s, probiotics have been widely used as biological veterinary drugs and feed additives in the prevention and treatment of enteritis and diarrhea in piglets, calves, lambs, poultry and other animals. At present, there are no domestically produced microecological preparations specifically for pet dogs. In clinical practice, human microecological preparations such as Zhengchangsheng and Probiotics are often used to regulate the balance of intestinal flora in dogs, but the effe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/74A61P1/12C12R1/10A61K35/742
Inventor 倪学勤曾东李洁萍曾燕舒刚景波彭焕伟
Owner SICHUAN AGRI UNIV
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