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Method for synchronously realizing water-phase transfer and nucleus targeting of hydrophobic nanoparticles

A nanoparticle, hydrophobic technology, applied in nanotechnology, nanotechnology, chemical instruments and methods, etc., can solve the problems of inability to achieve water phase conversion, complex process, etc., and achieve the effect of easy operation, simple process and good dispersibility.

Inactive Publication Date: 2013-04-03
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that in the prior art it is impossible to transfer the water phase, endow the granule with nuclear targeting; and modify the process of the nucleus localization signal peptide on the surface of the granule. Methods for Aqueous Phase Transfer and Nucleus Targeting

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  • Method for synchronously realizing water-phase transfer and nucleus targeting of hydrophobic nanoparticles
  • Method for synchronously realizing water-phase transfer and nucleus targeting of hydrophobic nanoparticles

Examples

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Embodiment 2

[0020] Example 2, preparation of dodecyldimethylbenzyl ammonium chloride modified CdS nanoparticles.

[0021] Get 1ml of chloroform solution (concentration of CdS contained therein is 0.5mg / ml) of CdS nanocrystal (average particle diameter is 3.2nm) coated with oleic acid, under magnetic stirring, slowly add to 40ml dodecyl di methyl benzyl ammonium chloride in aqueous solution (the concentration of dodecyl dimethyl benzyl ammonium chloride in this solution is 0.2M), and then continue magnetic stirring for 24 hours. After 24 hours, use an ultrafiltration centrifuge tube with a molecular weight cut-off of 10 KDa to centrifuge several times (4000 rpm, 10 min / time) to remove excess dodecyldimethylbenzyl ammonium chloride. The final dodecyldimethylbenzylammonium chloride-modified CdS nanoparticles have good dispersion in water, and the average size is about 5.4nm. After co-cultivating CdS nanoparticles modified with dodecyldimethylbenzyl ammonium chloride with human liver cancer ...

Embodiment 3

[0022] Embodiment 3, magnetic Fe modified by dodecyl dimethyl benzyl ammonium chloride 3 o 4 Preparation of nanoparticles.

[0023] Magnetic Fe coated with oleic acid 3 o 4 1ml of chloroform solution of nanoparticles (average particle diameter is 5nm) (the magnetic Fe contained therein 3 o 4 The concentration is 5mg / ml), under mechanical stirring, slowly join in the aqueous solution of 40ml dodecyl dimethyl benzyl ammonium chloride (dodecyl dimethyl benzyl ammonium chloride in this solution concentration of 0.2M), then mechanical stirring was continued for 24 hours. After 24 hours, use an ultrafiltration centrifuge tube with a molecular weight cut-off of 10 KDa to centrifuge several times (4000 rpm, 10 min / time) to remove excess dodecyldimethylbenzyl ammonium chloride. The final dodecyl dimethyl benzyl ammonium chloride modified magnetic Fe 3 o 4The nanoparticles are well dispersed in water with an average size of about 7.6nm. And the dodecyl dimethyl benzyl ammonium ...

Embodiment 7

[0030] Example 7, CuInS modified by cetyltrimethylammonium bromide 2 - Preparation of ZnS nanoparticles

[0031] CuInS coated with oleic acid 2 -ZnS nanoparticles (average particle diameter is 50nm) in chloroform solution 1ml (CuInS contained therein 2 -ZnS concentration is 5mg / ml), under magnetic stirring, slowly join in the aqueous solution of 30ml cetyltrimethylammonium bromide (concentration of cetyltrimethylammonium bromide in this solution is 0.1M), and then continue magnetic stirring for 72 hours. After 72 hours, use an ultrafiltration centrifuge tube with a molecular weight cut-off of 10 KDa to centrifuge several times (4000 rpm, 10 min / time) to remove excess cetyltrimethylammonium bromide. The final cetyltrimethylammonium bromide modified CuInS 2 -ZnS nanoparticles are well dispersed in water with an average size of about 62nm. CuInS modified with cetyltrimethylammonium bromide 2 -ZnS nanoparticles were co-cultured with human umbilical vein endothelial HUVEC cel...

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Abstract

The invention relates to a method for synchronously realizing water-phase transfer and nucleus targeting of hydrophobic nanoparticles, and belongs to the technical field of nanometer materials. The method comprises the general steps of adding a proper amount of organic solution with the hydrophobic nanoparticles to the aqueous solution containing a surface active agent with a certain concentration; stirring for an appropriate time; and then separating to remove residual surface active agent molecules, wherein the utilized surface active agent molecules contain quaternary ammonium salts at the hydrophobic ends. According to the method, such a surface active agent is adopted and used for modifying the hydrophobic nanoparticles, thus the water-phase transfer of the hydrophobic nanoparticles can be realized, and the nanoparticles show remarkable effect of nucleus targeting without requiring any additional modifications or treatments after the water-phase transfer.

Description

technical field [0001] The invention introduces a method for simultaneously realizing water phase transfer and cell nucleus targeting of hydrophobic nanoparticles, and belongs to the technical field of nanometer materials. Background technique [0002] The rapid development of nanotechnology in today's world makes it possible to precisely control the positioning of nanoparticles in specific organs, specific cells, and even specific organelles in complex organisms. Among them, at the subcellular level, designing new functional nanomaterials with specific organelle targeting is challenging but of far-reaching significance. The nucleus is the most important organelle in the cell, and it is also the target of many anticancer drugs (such as doxorubicin, cisplatin, etc.). The preparation of nuclear-targeted functional nanomaterials will play an extremely important role in the in-depth study of major life activities in the nucleus and the development of new diagnostic and therapeu...

Claims

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Application Information

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IPC IPC(8): C01G11/02C01G49/08C01G15/00C01B19/00C01G21/21C01G9/08B22F1/02B82Y30/00
Inventor 曹传宝王美娜王艳丽白驹
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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