Benzene composite degrading microbe and immobilized benzene composite microbial inoculum, and preparation method thereof

A technology of compound bacterial agent and benzene series, which is applied in the field of environmental pollution control, can solve the problems of large loss of microbial activity, easily broken carrier particles, large mass transfer resistance, etc., and achieves improved mechanical strength and chemical stability, and resistance to biodegradation. Enhanced performance and low mass transfer resistance

Inactive Publication Date: 2013-04-03
CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional immobilization method has the defects that the carrier particles are easily broken, the

Method used

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  • Benzene composite degrading microbe and immobilized benzene composite microbial inoculum, and preparation method thereof
  • Benzene composite degrading microbe and immobilized benzene composite microbial inoculum, and preparation method thereof

Examples

Experimental program
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Effect test

Example Embodiment

[0023] Example 1 Breeding of BTEX composite degradation bacteria

[0024] The breeding of BTEX composite degrading bacteria agent includes the following steps:

[0025] (1) Screening of strains, take 20 mL of landfill leachate, place it in a triangular flask filled with glass beads and 80 mL of distilled water, incubate at 30°C, 115 rpm for 12 hours, and take 3 mL of bacterial suspension The solution was inoculated in a screening medium containing a total concentration of 50 mg / L BTEX, and cultured at 30°C and 115 rpm for 7 days.

[0026] (2) Take 3 mL of the bacterial solution obtained in step (1), centrifuge at 10000 rpm for 7 minutes, wash 2-3 times with sterile saline, and inoculate it into a screening medium containing 50 mg / L BTEX at 30°C, Cultivate in a shaker at 115 rpm for 4 days, pass again, and domesticate 10 times. During the domestication process, the total concentration of BTEX is gradually increased. The total concentration of BTEX in the first and second generations...

Example Embodiment

[0040] Example 2 Preparation method of immobilized benzene series compound bacterial agent

[0041] Material preparation:

[0042] Prepare polyvinyl alcohol into a solution with a concentration of 250 g / L, and then add glycerin until the volume fraction of glycerin is 1%, which is recorded as solution A.

[0043] Prepare 1.0 mol / L KH 2 PO 4 -K 2 HPO 4 Buffer solution, marked as solution B, for use.

[0044] The sawdust is crushed into a particle size of 0.5 mm-1.0 mm as an immobilized carrier.

[0045] The BTEX composite degrading bacteria prepared in Example 1, add 0.1g of the mixed bacteria to 100 mL of screening medium containing 200 mg / L BTEX, and the final concentration of the mixed bacteria is 1 g / L, and cultivate to OD 600 =0.8, then centrifuge at 12000×g for 10 min, collect the precipitate, wash 2-3 times with physiological saline, and then centrifuge at 12000×g for 10 min to collect the precipitate;

[0046] The immobilization method of BTEX complex bacteria agent includes the ...

Example Embodiment

[0049] Example 3

[0050] Degradation effect of immobilized BTEX compound bacteria on BTEX in landfill leachate:

[0051] Place 1 g of the immobilized carrier prepared in Example 2 in 100 mL of screening medium containing 200 mg / L BTEX, and incubate in a shaker at 30°C and 115 rpm, and measure the BTEX concentration every 12 hours. Table 3 shows.

[0052] Table 3. Degradation rate of BTEX by compound bacteria

[0053]

[0054] As shown in Table 3, the degradation rate of BTEX within 2 days of mixed bacteria is above 90%. Therefore, the immobilized BTEX composite bacterial agent obtained by the present invention has the characteristics of high efficiency and rapid degradation of BTEX, and is suitable for the removal of BTEX in the landfill leachate and the treatment of water bodies polluted by BTEX.

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Abstract

The invention relates to a benzene composite degrading microbe and an immobilized benzene composite microbial inoculum, and a preparation method thereof. The benzene composite degrading microbe is a mixture of Pseudomonas putida RW10S2, Pseudomonas stutzeri, Pseudomonas putida ND6 and Pseudomonas putida BIRD-1. The benzene composite degrading microbe can be immobilized on a floatable degradable immobilized vector to obtain the immobilized benzene composite microbial inoculum. The preparation method is simple; the prepared immobilized benzene composite microbial inoculum has favorable degradation effect on benzenes, and the degradation rate for benzenes within two days is higher than 90%; and the immobilized benzene composite microbial inoculum can be widely used for removing benzenes in garbage leachate, and can also be used for treating benzene-polluted water bodies.

Description

technical field [0001] The invention relates to the field of environmental pollution control, in particular to a benzene series compound degradation bacterium, and also relates to an immobilized benzene series compound bacterial agent and a preparation method thereof. Background technique [0002] BTEX (benzene series) is a class of volatile monocyclic aromatic hydrocarbon compounds, including benzene (benzene), toluene (toluene), ethylbenzene (ethylbenzene) and xylene (xylene), mainly present in crude oil and petroleum products, As an industrial raw material, it is widely used in industries such as pesticides, plastics and fiber synthesis. Because BTEX itself is carcinogenic and mutagenic, and can produce biological toxicity even at very low concentrations, this class of compounds has become the focus of environmental concern. These compounds are volatile and easily enter the atmosphere, participate in photochemical reactions, promote the formation of ozone, and further ag...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/08C12N11/02C12R1/40C12R1/38
Inventor 周月明乔婧唐薇李斗尹雅洁王洋清雷禹黄亦存宋立岩
Owner CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
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