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Benzene composite degrading microbe and immobilized benzene composite microbial inoculum, and preparation method thereof

A technology of compound bacterial agent and benzene series, which is applied in the field of environmental pollution control, can solve the problems of large loss of microbial activity, easily broken carrier particles, large mass transfer resistance, etc., and achieves improved mechanical strength and chemical stability, and resistance to biodegradation. Enhanced performance and low mass transfer resistance

Inactive Publication Date: 2013-04-03
CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional immobilization method has the defects that the carrier particles are easily broken, the mass transfer resistance is large, the gas production is floating up, and the microbial activity is greatly lost.

Method used

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  • Benzene composite degrading microbe and immobilized benzene composite microbial inoculum, and preparation method thereof
  • Benzene composite degrading microbe and immobilized benzene composite microbial inoculum, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The breeding of embodiment 1 BTEX compound degrading bacterial agent

[0024] The breeding of BTEX compound degrading bacterial agent comprises the following steps:

[0025] (1) Screening of bacterial strains. Take 20 mL of landfill leachate, put it in a conical flask filled with glass beads and 80 mL of distilled water, cultivate it at 30°C and 115 rpm for 12 hours, and take 3 mL of bacterial suspension The liquid was inoculated in the screening medium containing 50 mg / L BTEX at a total concentration, and cultured at 30°C and 115 rpm for 7 days.

[0026](2) Take 3 mL of the bacterial solution obtained in step (1), centrifuge it at 10,000 rpm for 7 minutes, wash it 2-3 times with sterilized normal saline, inoculate it into the screening medium containing 50 mg / L BTEX, and incubate at 30°C, Cultured in a shaker at 115 rpm for 4 days, subcultured again, and domesticated 10 times in total. During the domestication process, the total concentration of BTEX increased seque...

Embodiment 2

[0040] Embodiment 2 The preparation method of immobilized benzene series complex bacterial agent

[0041] Material preparation:

[0042] Polyvinyl alcohol was prepared into a solution with a concentration of 250 g / L, and then glycerin was added until the volume fraction of glycerin was 1%, which was designated as solution A.

[0043] Prepare 1.0 mol / L of KH 2 PO 4 -K 2 HPO 4 The buffer solution, recorded as solution B, is set aside.

[0044] Sawdust is crushed into a particle size of 0.5 mm-1.0 mm as an immobilized carrier.

[0045] With the BTEX composite degrading bacteria prepared in Example 1, take 0.1 g of mixed bacteria and add them to 100 mL screening medium containing 200 mg / L BTEX, the final concentration of mixed bacteria is 1 g / L, and cultivate to OD 600 =0.8, then centrifuged at 12000×g for 10 min to collect the precipitate, washed 2-3 times with normal saline, and then centrifuged at 12000×g for 10 min to collect the precipitate;

[0046] The immobilizati...

Embodiment 3

[0050] Degradation effect of immobilized BTEX composite bacterial agent on BTEX in landfill leachate:

[0051] 1 g of the immobilized carrier prepared in Example 2 was placed in 100 mL of screening medium containing 200 mg / L BTEX, cultivated in a shaker at 30°C and 115 rpm, and the concentration of BTEX was measured every 12 hours, such as Table 3 shows.

[0052] Table 3. Degradation rate of BTEX by compound bacterial agents

[0053]

[0054] As shown in Table 3, the degradation rate of BTEX within 2 days of mixed bacteria was above 90%. Therefore, the immobilized BTEX composite bacterial agent obtained in the present invention has the characteristics of high efficiency and rapid degradation of BTEX, and is suitable for the removal of BTEX in landfill leachate and the treatment of water bodies polluted by BTEX.

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Abstract

The invention relates to a benzene composite degrading microbe and an immobilized benzene composite microbial inoculum, and a preparation method thereof. The benzene composite degrading microbe is a mixture of Pseudomonas putida RW10S2, Pseudomonas stutzeri, Pseudomonas putida ND6 and Pseudomonas putida BIRD-1. The benzene composite degrading microbe can be immobilized on a floatable degradable immobilized vector to obtain the immobilized benzene composite microbial inoculum. The preparation method is simple; the prepared immobilized benzene composite microbial inoculum has favorable degradation effect on benzenes, and the degradation rate for benzenes within two days is higher than 90%; and the immobilized benzene composite microbial inoculum can be widely used for removing benzenes in garbage leachate, and can also be used for treating benzene-polluted water bodies.

Description

technical field [0001] The invention relates to the field of environmental pollution control, in particular to a benzene series compound degradation bacterium, and also relates to an immobilized benzene series compound bacterial agent and a preparation method thereof. Background technique [0002] BTEX (benzene series) is a class of volatile monocyclic aromatic hydrocarbon compounds, including benzene (benzene), toluene (toluene), ethylbenzene (ethylbenzene) and xylene (xylene), mainly present in crude oil and petroleum products, As an industrial raw material, it is widely used in industries such as pesticides, plastics and fiber synthesis. Because BTEX itself is carcinogenic and mutagenic, and can produce biological toxicity even at very low concentrations, this class of compounds has become the focus of environmental concern. These compounds are volatile and easily enter the atmosphere, participate in photochemical reactions, promote the formation of ozone, and further ag...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/08C12N11/02C12R1/40C12R1/38
Inventor 周月明乔婧唐薇李斗尹雅洁王洋清雷禹黄亦存宋立岩
Owner CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
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