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Mixed enzyme for degrading celluloses

A technology for degrading cellulose and cellulose, applied in the direction of enzymes, enzymes, applications, etc., to achieve the effects of easy use, high effective content, and low price

Inactive Publication Date: 2013-04-03
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

At present, there is no product on the market that combines different cellulase enzymes to achieve efficient utilization of cellulase

Method used

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Experimental program
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Effect test

Embodiment Construction

[0010] Cellulose C used in the present invention 1 The enzyme is endoglucanase, which can act on the non-crystalline region inside cellulose, randomly hydrolyze β-1, 4 glycosidic bonds, cut off long-chain cellulose molecules, form small molecular cellulose, and decompose new non-crystalline reducing end. The second Cx enzyme is exoglucanase, which binds to the free end, acts on the non-reducing end to cut off the cellobiose unit, and decomposes a cellobiose molecule. The third cellulase is β2-glucosidase, which acts to hydrolyze cellobiose to form glucose molecules that can be absorbed by the animal body. These three cellulase enzymes can be selected from commercially available products, for example, products from Shanghai Chenyi Biotechnology Co., Ltd., Su Kehan ​​(Weifang) Bioengineering Co., Ltd., and Hersbit Biotechnology Co., Ltd. The present invention uses the pepsin-trypsin two-step digestion method to measure the digestibility of dry matter and crude protein and the ...

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Abstract

The invention provides a mixed enzyme for degrading celluloses. The mixed enzyme comprises a cellulose C1 enzyme, a cellulose Cx enzyme and a cellulose beta2 glucosaccharase in mass ratios of 3:3:2. In order to increase the effect of the mixed enzyme on degradation of the celluloses, bifidobacterium viable powder is added in the mixed enzyme. The mixed enzyme provided by the invention overcomes the disadvantage of incomplete functions of a single enzyme system, can increase the growth performance of animal production, nutrient digestibility and the cellulose using capability of animals and is multifunctional. The mixed enzyme provided by the invention is low in cost, convenient in use and easy to popularize in production practice. Animal experiments prove that the composite cellulase added in a feed can obviously increase the production performance of the animals, apparent digestibility of amino acids, ratio of feed to gain, and conventional nutrient digestibility.

Description

technical field [0001] The invention belongs to the field of animal husbandry feed processing, in particular to a mixed enzyme for degrading cellulose. Background technique [0002] Cellulase is one of the most commonly used enzyme preparations in livestock and poultry diets in my country. There are many companies that produce and sell cellulase. How to screen many cellulase products for different varieties and different growth stages? It is very necessary for pigs to choose economical and effective cellulase. Through long-term breeding and breeding of local pig breeds in my country, most of them have the advantages of being resistant to rough feeding and strong adaptability, and have formed the habit of using fibrous feed during the long-term feeding process. With the in-depth research on the physical and chemical properties and nutritional physiological effects of dietary fiber, it has been found that a certain level of dietary fiber can not only regulate the microecologic...

Claims

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Application Information

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IPC IPC(8): C12N9/42A23K1/165A23K1/16
Inventor 宋春阳姜建阳聂昌林倪良振远德龙
Owner QINGDAO AGRI UNIV
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