Tomato lipoxygenase family silent expression vector, and construction method and application thereof

Abstract

The invention discloses a tomato lipoxygenase family silent expression vector, and a construction method and application thereof. The silent expression vector contains a double-chain RNA (ribonucleic acid) expression unit, and comprises a promoter, a TomloxACD spliced gene forward segment, a TomloxACD spliced gene reverse segment and a terminator, wherein the TomloxACD spliced gene is spliced from TomloxA gene segment (SEQ ID No.1), TomloxC gene segment (SEQ ID No.2) and TomloxD gene segment (SEQ ID No.3). The silent expression vector is transformed into wild type tomato under the mediation of agrobacterium to obtain the transgenic positive plant; and the alpha-linolenic acid and linoleic acid content in the fruit is obviously increased as compared with that in the non-transgenic plant, which indicates that the silent expression vector can be used for breeding high-unsaturated-fatty-acid fruit tomato plants and has favorable application prospects in the aspects of improving the tomato fruit quality and enhancing the nutritive value of the tomato fruit.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and relates to a gene silencing expression vector, a construction method of the gene silencing expression vector, and an application of the gene silencing expression vector in plant breeding. Background technique [0002] tomato( Lycopersicon esculentum Mill.) is a kind of important crop of Solanaceae, is one of the most common fruits and vegetables cultivated in the whole world. Essential fatty acids are essential for the life activities of the body, but the body cannot synthesize them or the synthesis speed is too slow to meet the needs of the body. The polyunsaturated fatty acids that must be supplied by food mainly include α-linolenic acid and linoleic acid. Genetic engineering breeding has become an important branch in the field of plant breeding, showing great potential in the directional improvement of plant characteristics. In recent years, there are relatively many reports...

AI Technical Summary

Problems solved by technology

In recent years, there are relatively many reports on the use of transgenic technology to increase the resistance to diseases and insect pests, herbicides and salt and alkali resistance of tomato, but there is no report on the use of transgenic technology to increase the content of unsaturated fatty acids in tomato fruits.

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