Molecular detection method for Cry1Ac toxin non-recessive resistance cadherin cytomere deletion mutation of cotton bollworm

Abstract

The invention discloses a molecular detection method for CrylAc toxin non-recessive resistance cadherin cytomere deletion mutation of cotton bollworm. The detection method comprises the following three steps: 1, extracting a genome DNA of a cotton bollworm sample respectively; 2, performing polymerase chain reaction (PCR) amplification on a specific cadherin cytomere segment; and 3, performing agarose gel electrophoresis on the PCR product, and precisely identifying the genotype according to an electrophoretogram to determine the resistance gene frequency of different population. According to the method, no test bollworm needs to raise, so that labor force and resources are saved; and the genotype of the cotton bollworm can be detected quickly and precisely, a single bollworm test only needs several hours, the collection and identification for the frequency of resistant individuals of field population only need several weeks, and the identification result can timely provide correct guide for the effective resistance management of the cotton bollworm.

Description

technical field [0001] The invention relates to biotechnology, and relates to a molecular detection method for non-recessive resistance cadherin cytoplasmic region deletion mutation of Cry1Ac toxin in cotton bollworm. technical background [0002] Transgenic Bt cotton expressing Cry1Ac toxin protein has been popularized and applied in my country since 1997. At present, it has been planted on a large scale in the cotton areas of the Yellow River Basin and the Yangtze River Basin, accounting for about 75% of the national cotton planting area on average. Due to the high-intensity selection pressure of large-scale promotion and application of transgenic Bt cotton, its target pest cotton bollworm (Helicoverpa armigera) has developed resistance to Cry1Ac toxin, which is the biggest threat to the use of Bt cotton. The current research shows that the mutation of the intestinal parietal cell cadherin gene in the cotton bollworm is the main reason for the resistance. Cadherins are a ...

AI Technical Summary

Problems solved by technology

However, the above four resistance monitoring methods have obvious shortcomings in the monitoring of the resistance of cotton bollworm to Bt cotton, such as the resistance gene type cannot be detected, it is difficult to determine the appropriate diagnostic dose, and the corresponding resistant strains must be available indoors, which is costly. , takes a long time, etc., resulting in inaccurate and delayed resistance monitoring, which seriously affects the development and implementation of subsequent effective resistance control

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