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Preparation method of antimicrobial peptide loaded chitosan/gamma polyglutamic acid composite nanoparticles

A technology of composite nanoparticles and polyglutamic acid, which is applied in the field of medical engineering, can solve the problems of slow drug release, lower burst release rate, and not many nanoparticles, and achieve increased safety, reduced number of administrations, and simple operation Effect

Active Publication Date: 2013-04-17
山东润土节能环保工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are not many studies on the preparation of nanoparticles by chitosan and γ-polyglutamic acid through ion cross-linking. drug entrapment nanosystem

Method used

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  • Preparation method of antimicrobial peptide loaded chitosan/gamma polyglutamic acid composite nanoparticles
  • Preparation method of antimicrobial peptide loaded chitosan/gamma polyglutamic acid composite nanoparticles
  • Preparation method of antimicrobial peptide loaded chitosan/gamma polyglutamic acid composite nanoparticles

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Embodiment 1: the chitosan / γ-polyglutamic acid nanoparticle of load antimicrobial peptide to be prepared, specific weight percentage is: chitosan / γ-polyglutamic acid / antibacterial peptide=50 / 10 / 1, preparation The process is as follows:

[0023] ① Configuration of chitosan solution: Dissolve 50.0mg of chitosan (deacetylation degree is 85%, molecular weight: 150,000) in 100.0mL of 1% dilute acetic acid solution, stir at room temperature, and make 0.5mg / mL Chitosan acetate solution.

[0024] ② Mix 0.5mg / mL γ-polyglutamic acid (purity 92%, molecular weight 1 million) aqueous solution with 0.01mg / mL antimicrobial peptide aqueous solution at a volume ratio of 10:1, and keep stirring until faint blue fluorescence appears and stabilizes γ-polyglutamic acid / antimicrobial peptide composite nanoparticle solution is obtained; take an appropriate amount of γ-polyglutamic acid / antibacterial peptide composite nanoparticle solution, and drop it into a certain volume of chitosan dilute...

Embodiment 2

[0027] Embodiment 2: the chitosan / γ-polyglutamic acid nanoparticle of load antimicrobial peptide to be prepared, specific weight percent is: chitosan / γ-polyglutamic acid / antibacterial peptide=50 / 25 / 5, prepares The process is as follows:

[0028]① Configuration of chitosan solution: Dissolve 250.0 mg of chitosan (90% deacetylation degree, 880,000 molecular weight) in 100.0 mL of 2% dilute acetic acid solution, stir at room temperature, and prepare 2.5 mg / mL Chitosan acetate solution.

[0029] ②Mix 2.5mg / mL γ-polyglutamic acid aqueous solution (purity 95%, molecular weight 1.2 million) and 0.4mg / mL antimicrobial peptide aqueous solution in a volume ratio of 4:5, and keep stirring until blue fluorescence appears and exists stably , to obtain the γ-polyglutamic acid / antibacterial peptide composite nanoparticle solution; take an appropriate amount of γ-polyglutamic acid / antibacterial peptide composite nanoparticle solution, and drop it into a certain volume of chitosan acetate sol...

Embodiment 3

[0032] Embodiment 3: the chitosan / γ-polyglutamic acid nanoparticle of load antimicrobial peptide to be prepared, specific weight percentage is: chitosan / γ-polyglutamic acid / antibacterial peptide=50 / 50 / 50, prepare The process is as follows:

[0033] ① Configuration of chitosan solution: Dissolve 500.0 mg of chitosan (95% deacetylation degree, 1.5 million molecular weight) in 100.0 mL of 3% dilute acetic acid solution, stir at room temperature, and prepare 5.0 mg / mL Chitosan acetate solution.

[0034] ②Mix 5.0 mg / mL γ-polyglutamic acid aqueous solution (purity 99%, molecular weight 1.45 million) and 10.0 mg / mL antimicrobial peptide aqueous solution in a 2:1 volume ratio, and keep stirring until blue fluorescence appears and exists stably , to obtain the γ-polyglutamic acid / antibacterial peptide composite nanoparticle solution; take an appropriate amount of γ-polyglutamic acid / antibacterial peptide composite nanoparticle solution, and drop it into a certain volume of chitosan ac...

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Abstract

The invention discloses a preparation method of antimicrobial peptide loaded chitosan / gamma polyglutamic acid composite nanoparticles. The preparation method is characterized by comprising the following steps: (1) mixing 0.5-5.0mg / mL gamma-polyglutamic acid aqueous liquor with 0.01-10.0mg / mL antimicrobial peptide aqueous liquor, and continuously stirring till blue-fluorescence occurs and exists stably to obtain a gamma polyglutamic acid / antimicrobial peptide composite nanoparticle liquor; and (2) dropping the gamma polyglutamic acid / antimicrobial peptide composite nanoparticle liquor into the polyglutamic acid aqueous liquor, wherein the concentration of chitosan in the polyglutamic acid aqueous liquor is 0.5-5.0mg / mL and the volume concentration of acetic acid is 1-3%; continuously stirring till blue-fluorescence occurs and exists stably, freezing and drying precipitate to obtain the antimicrobial peptide loaded chitosan / gamma polyglutamic acid composite nanoparticles. According to the antimicrobial peptide loaded chitosan / gamma polyglutamic acid composite nanoparticles prepared by the invention, initial sudden release of antimicrobial peptid can be reduced, and the releasing time of a medicine is effectively prolonged, so that the antimicrobial peptide is efficiently used.

Description

(1) Technical field [0001] The invention relates to a preparation method of a long-acting and safe antibacterial nanometer material, in particular to a preparation method of chitosan / γ-polyglutamic acid composite nanoparticle loaded with antibacterial peptide, and belongs to the technical field of medical engineering. (2) Background technology [0002] Antimicrobial peptides, also known as antimicrobial peptides or peptide antibiotics, are widely distributed in animals and plants and are part of the natural immune defense system. Antimicrobial peptides not only have broad-spectrum antibacterial ability, but also have effects on fungi, viruses and cancer cells. In recent years, due to the increasingly serious problems of drug abuse, drug residues and bacterial drug resistance, which has aroused people's attention to food and the environment, more and more countries have begun to call for the prohibition of the abuse of antibiotics. Antimicrobial peptides have aroused great r...

Claims

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Application Information

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IPC IPC(8): A61K47/48A61K38/17A61K9/14A61P31/04
Inventor 孙燕柳永许尧兴符思达曹勇王磊
Owner 山东润土节能环保工程有限公司
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