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Preparation method of yeast beta-D-glucan

A technology of glucan and yeast, which is applied in the field of preparation of yeast β-D-glucan, can solve problems such as limited industrial scale production, environmental pollution, and damage to human health, and achieves significant economic benefits and environmental protection significance. The preparation conditions are mild and the effect of realizing industrial scale production

Active Publication Date: 2013-04-17
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many methods for preparing Saccharomyces cerevisiae β-D-glucan, most of them are limited in industrial scale production
At present, the method widely used in my country to industrialize the production of Saccharomyces cerevisiae β-glucan is mainly the alkaline method. Although the product prepared by the alkaline method has high purity, the alkaline method will destroy the structure of β-D-glucan and make the yield low. , and a large amount of alkali will pollute the environment and damage human health
The method of preparing high-purity yeast β-D-glucan suitable for industrial production by using mild conditions such as non-strong acid and strong alkali has not been reported yet.

Method used

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Experimental program
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Effect test

Embodiment 1

[0048] Embodiment 1, preparation of yeast β-D-glucan

[0049] (1) Autolysis: use Saccharomyces cerevisiae with high β-D-glucan content (FFLM2.0016, its preservation number is CGMCCNo.3730) as raw material, wash with water, centrifuge to remove impurities, and add water at a volume ratio of 1:5 to prepare Add NaCl to the suspension to make the final concentration 3%, place it in a constant temperature water bath shaker at pH 5.0 and 55°C to induce autolysis for 24 hours, raise the temperature to 85°C, keep it warm for 15 minutes to inactivate the enzyme activity, centrifuge, and wash with water Precipitate 3 times;

[0050] (2) High-temperature extraction: Add 0.02mol / L, pH7.5 phosphate buffer to the precipitate obtained in step (1) at a specific mass ratio of 1:5 to prepare a suspension, shake in a constant temperature water bath at 95°C Extract at high temperature in a container for 4 hours, centrifuge, wash and precipitate with water twice;

[0051] (3) High-pressure micro-j...

Embodiment 2

[0062] Embodiment 2, preparation of yeast β-D-glucan

[0063] (1) Autolysis: use Saccharomyces cerevisiae with high β-D-glucan content (FFLM2.0016, its preservation number is CGMCCNo.3730) as raw material, wash with water, centrifuge to remove impurities, and add water at a volume ratio of 1:20 to prepare Add NaCl to the suspension to make the final concentration 3%, place it in a constant temperature water bath shaker at pH 5.0 and 55°C to induce autolysis for 24 hours, raise the temperature to 100°C, keep it warm for 10 minutes to inactivate the enzyme, centrifuge, and wash with water Precipitate 3 times;

[0064] (2) High-temperature extraction: Add 0.02mol / L, pH7.5 phosphate buffer solution to the precipitate obtained in step (1) at a specific mass ratio of 1:20 to prepare a suspension, shake in a constant temperature water bath at 95°C Extract at high temperature in a container for 4 hours, centrifuge, wash and precipitate with water twice;

[0065] (3) High-pressure mi...

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Abstract

The invention disclose a preparation method of yeast beta-D-glucan. The method comprises the following steps that (1) a brewer yeast is added to water to form a suspension a; NaC1 is added to the suspension a; induced autolysis, enzymatic inactivation and then centrifugation are conducted; a sediment a is obtained; (2) a phosphate buffer is added to the sediment a to form a suspension b; a sediment b is obtained by extraction and centrifugation; (3) water is added to the sediment b to form a suspension c; the suspension c is subjected to microjet homogenization treatment and then centrifugation to form a sediment c; (4) a phosphate buffer b is added to the sediment c to form a suspension d; enzyme is added to the suspension d for enzymolysis; then enzymatic inactivation treatment is conducted; (5) an enzymatic hydrolysate treated in Step (4) is subjected to the microjet homogenization treatment and then centrifugation to form a sediment d; and (6) the sediment d is dehydrated and dried in sequence to form yeast beta-D-glucan. The method can realize comprehensive utilization of industrial by-products, namely waste beer yeasts and increase the utilization ratio and added values of the industrial by-products, and has significant economic benefits and environmental protection significance.

Description

technical field [0001] The invention relates to a preparation method of yeast β-D-glucan. Background technique [0002] β-D-glucan is a type of polysaccharide widely found in bacteria, fungi, algae and plants, and one of its main sources is Saccharomyces cerevisisae. Yeast β-D-glucan accounts for about 50% of the yeast cell wall, and its intramolecular polyols interact to form a dense triple helical structure with a diameter of generally 20-80 μm. Yeast β-D-glucan has many physiological functions such as enhancing immunity, anti-inflammation, antibacterial, anti-infection, anti-virus, anti-cancer, lowering cholesterol, preventing radiation, healing wounds, etc. It is a good biological effect regulator[ BRMs]. my country has abundant resources of Saccharomyces cerevisiae, such as waste beer yeast. In recent years, my country's beer industry has developed rapidly, and its annual output has exceeded 40 million tons. As one of the main by-products of the beer industry, beer w...

Claims

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Application Information

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IPC IPC(8): C08B37/02
Inventor 王强刘红芝刘丽胡晖高洁
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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