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Extraction method of microalga grease

A technology of microalgae oil and extraction method, which is applied in the direction of oil/fat production, fat production, etc., can solve the problems of complex composition and many side reactions, and achieves the effect of facilitating diffusion, reducing process links and saving energy consumption.

Inactive Publication Date: 2013-04-17
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method does not describe oil extraction, but the process of preparing biodiesel by chemical catalysis. Due to the presence of a large number of cell lysates in the reaction, the composition is complex and there are many side reactions.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A kind of chlorella vulgaris was selected and cultured in a 10L tank for 15 days. At the later stage of microalgae culture, according to the established relationship between OD and cell dry weight, the concentration of microalgae was calculated to be 1.02g / L (dry weight count).

[0022] (1) Pretreatment of microalgae raw materials: Take 1L of microalgae culture solution, adjust the pH of the microalgae culture solution to 12 with NaOH, and let it stand for 30 minutes. The algae liquid at the bottom of the culture tank was taken for centrifugation, and the centrifugation speed was controlled to 5000rpm, and the centrifugation time was 5min.

[0023] (2) Oil extraction: Add 60ml of n-hexane to the algae mud, shake and mix well, put it into the microwave generator, turn on the microwave generator, use intermittent microwave treatment, the microwave frequency is 2450MHz, and the microwave power is set to 1000W. The temperature was set at 65°C and the time was 2h.

[0024]...

Embodiment 2

[0025] Embodiment 2 Utilize the method of the present invention to the extraction of botrytis oil

[0026] A kind of botrytis (Botryococcus braunii) was selected and cultured in a 10L tank for 20 days. At the later stage of microalgae culture, according to the established relationship between OD and cell dry weight, the concentration of microalgae was calculated to be 1.41g / L (dry weight basis ).

[0027] (1) Collection of microalgae cells: Take 1.5L microalgae culture solution, adjust the pH of the microalgae culture solution to 12 with NaOH, and let it stand for 30 minutes. The algae liquid at the bottom of the cultivation tank was taken for centrifugation, the centrifugal speed was 10000rpm, and the centrifugation time was 10min.

[0028] (2) Oil extraction: add 120ml of petroleum ether to the micro-sludge, shake and mix well, turn on the microwave generator, adopt intermittent microwave treatment, microwave frequency is 915MHz, set microwave power to 2000W, temperature to...

Embodiment 3

[0029] Embodiment 3 Utilize the present invention to the extraction of algal flour oil

[0030] (1) Pretreatment of dry microalgae powder: Weigh 2.5 g of diatom algae powder that has been oven-treated at 70°C for 24 hours, add 30 ml of methanol after cooling.

[0031] (2) Oil extraction: Add 60ml of n-hexane to the mixture of algae powder and methanol, shake and mix well, then turn on the microwave generator, adopt intermittent microwave treatment, the microwave frequency is 915MHz, set the microwave power to 2000W, and set the temperature to 60°C, the time is 2h.

[0032](3) Recovery of solvent: After the reaction, methanol and n-hexane are recovered respectively, and finally the oil mixture is obtained. The calculated oil yield is 24.8%.

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Abstract

The invention discloses an extraction method of microalga grease. The extraction method comprises the following steps of: with alga ooze or dry powdery microalgae as raw materials, smashing pretreated microalgae in a microwave radiation manner, and meanwhile, extracting the microalgae by using an organic solvent; and after the extraction is ended, separating all substances to obtain the microalga grease. The microwave frequency can be any one of 2450MHz, 915MHz and 896MHz, and the microwave power is set to be 1-50kW / L. The method provided by the invention has the advantages of simple operation process, short extraction time, low energy consumption, strong raw material adaptability, high microalga grease yield and the like.

Description

technical field [0001] The invention relates to a method for extracting microalgae oil. Background technique [0002] Microalgae refers to tiny algae, which belong to aquatic plants. They have the characteristics of wide distribution, large biomass, high photosynthetic efficiency, strong environmental adaptability, short growth cycle, and high biomass yield. Microalgae can use the photosynthetic reaction system, through the CO 2 The fixation of light energy into chemical energy is stored in the body cells in the form of organic matter such as oil or starch, and its solar energy conversion efficiency can reach 3.5%. Microalgae is a potential resource for the production of pharmaceuticals, fine chemicals and new fuels. With the shortage of resources in human society and the increasingly severe environmental problems, the use of microalgae for the development of biodiesel and some fossil energy substitutes can overcome the need for petroleum resources. The disadvantage of non...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/10C11B1/04
Inventor 张霖师文静李晓姝王领民
Owner CHINA PETROLEUM & CHEM CORP
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