Preparation method of high-efficient degrading bacterium agent for phthalate ester environmental hormone
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A technology of phthalic acid and environmental hormones, applied in the field of environmental biology
Inactive Publication Date: 2013-04-17
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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Problems solved by technology
There is no literature report on the preparation of PAEs degrading bacteria by using sweet potato starch wastewater
Method used
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Embodiment 1
[0021] Embodiment 1: the preparation of phthalate degrading bacterial agent
[0022] (1) Medium preparation:
[0023] 1) Strain preservation medium (solid, 1L): sweet potato starch wastewater 1L, yeast extract 5g, sodium chloride 5g, agar 20g, adjust pH to 7.0-7.5;
[0025] 3) Seed medium (liquid, 10L): K 2 HPO 4 38g, KH 2 PO 4 15g, (NH 4 ) 2 SO 4 10g, MgCl 2 1.6g, CaCl 2 0.2g, Na 2 MoO 4 2H 2 O 0.024g, FeCl 3 0.018g, MnCl 2 2H 2 O 0.015g, DBP 5g, sweet potato starch wastewater 1L, add water to 10L, adjust pH=7.0-7.5.
[0026] 4) Fermentation medium (liquid, 200L): 200L of sweet potato starch wastewater, adjust the pH to 7.0-7.5;
[0027] All the above media were sterilized at 115°C for 15-30min.
[0028] 2. Strain activation: Pick PAEs-degrading bacteria to the strain preservation medium, streak and pick a single c...
Embodiment 2
[0032] Embodiment 2: the degradation effect of PAEs degrading bacteria agent to dibutyl phthalate
[0033] 1) Inoculate 1 ml of bacterial agent in 100 ml of seed medium containing 500 mg / L dibutyl phthalate; the one without inoculation is used as the control group; culture at 30° C. on a shaking table at 150 r / min.
[0034]2) After 40 hours, sample was extracted, and the content of dibutyl phthalate was determined by high performance liquid chromatography. The treatment group could not detect the presence of dibutyl phthalate, while the control group had almost no degradation.
Embodiment 3
[0035] Embodiment 3: The degradation effect of PAEs degrading bacteria agent to phthalic acid
[0036] 1) Inoculate 1 ml of bacterial solution in 100 ml of seed medium containing 300 mg / L phthalic acid; the one without inoculation is used as the control group; culture at 30° C., 150 r / min on a shaker.
[0037] 2) After 36 hours, the sample was extracted and the content of phthalic acid was determined by high performance liquid chromatography. The presence of phthalic acid was not detected in the treatment group, while the control group had almost no degradation.
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Abstract
The invention relates to a Gordonia sp. QH-11 bacterial strain which is separated from activated sludge and can efficiently degrade phthalate ester environmental hormone, provides a preparation method of the bacterium agent, and belongs to the field of environmental biotechnology. The bacterial strain is preserved in China General Microbiological Culture Collection Center with a preservation number of CGMCC No. 5276; the 16S rDNA gene sequence of the bacterial strain has been submitted to GenBank database with an accession number of JN641798. The production method of the bacterium agent comprises the following 5 steps of: culture medium preparation, bacterial strain activation, liquid seed preparation, liquid fermentation, and bacterium agent preparation, wherein the fermentation medium adopts sweet potato starch waste water as a main raw material. The degrading bacterium agent produced by the technical scheme of the invention has low production cost, can efficiently degrade phthalate ester environmental hormone in the environment, and can protect ecological environment and human health.
Description
technical field [0001] The invention belongs to the technical field of environmental biology, and relates to a bacterial strain (Gordonia sp. QH-11) capable of efficiently degrading phthalate-type environmental hormones, and a preparation method thereof. technical background [0002] Phthalate, also known as phthalate, abbreviated as PAEs, is a general term for esters formed by combining phthalic acid with corresponding alcohols. It is generally a colorless oily viscous liquid, which is difficult to dissolve in water and easily soluble Based on organic solvents, it is not easy to volatilize at room temperature, with low cost, many varieties and large output. It is the most common plasticizer in the plastic industry. Commonly used varieties in my country are: dioctyl phthalate (DOP), diisooctyl phthalate (DEHP), dibutyl phthalate (DBP), butyl benzyl phthalate (BBP) , Dinonyl phthalate (DNP), etc. In recent years, with the increase of industrial production and the use of plas...
Claims
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Application Information
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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/28
Inventor 白志辉金德才王萍常冬冬高莎庄国强
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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