Bacillus subtilis capable of producing high-yield protease and application of bacillus subtilis
A Bacillus subtilis, protein technology, applied in application, bacteria, microorganism-based methods, etc., can solve the problems of weak practical application effect, easy to contaminate bacteria, industrialization development limitation, etc. Effect
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[0013] Example 1
[0014] Obtaining of Bacillus subtilis Hzd-bs-001 and testing its culture conditions
[0015] 1. Screening and identification of Bacillus subtilis Hzd-bs-001
[0016] 1) Screening of Bacillus subtilis Hzd-bs-001
[0017] (1) Weigh 1g of soil from the soil of Tsingtao Brewery's long-term accumulation of beer lees and place it in 100ml of sterile water, stir evenly with a glass rod, and then shake on a shaker for 30 minutes to disperse the microbial cells, and let it stand for 20s~30s , Its aqueous solution is 10 -2 Diluent, and then pipette 1ml of the diluent into a 9ml test tube of sterile water, shake and mix, that is 10 -3 Diluent, and then draw 1ml 10 -3 The diluent is shaken and mixed in 9ml sterile water, which is 10 -4 Diluent, made 10 -2 , 10 -3 , 10 -4 Serial dilutions.
[0018] (2) Number the culture medium plate (3 repeats for each number), dip a loop of each series of dilutions with a sterile inoculation loop, and streak it on the solidified medium plate fo...
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[0031] Example 2
[0032] Salt tolerance test of strains
[0033] Bacillus subtilis Hzd-bs-001 was cultured in different salinity fermentation media at 5% inoculum under aseptic conditions, 230r / min, 35℃ shaking culture, and its OD600 value was measured every 2h, as shown in Table 2. Show. The test results show that Bacillus subtilis Hzd-bs-001 has strong salt tolerance and is suitable for feed production requirements.
[0034] Table 2 Salt tolerance test of Bacillus subtilis Hzd-bs-001
[0035]
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[0036] Example 3
[0037] Temperature tolerance test of strain
[0038] Bacillus subtilis Hzd-bs-001 was cultured in the same fermentation medium at a rate of 5% under aseptic conditions, 230r / min, and shaking at different temperatures, and its OD600 value was measured every 2h, as shown in Table 2. . The test results indicate that Bacillus subtilis Hzd-bs-001 grows most vigorously at 35°C, and also grows better at 45°C, which is suitable for feed production requirements.
[0039] Table 3 Temperature tolerance test of Bacillus subtilis Hzd-bs-001
[0040]
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