Monitoring system for in-vitro cell photostimulation array device

Abstract

The invention discloses a monitoring system for an in-vitro cell photostimulation array device, which is used for monitoring a culture state of photosensitive cells under photostimulation, and comprises a control module, a driving module, an LED (light-emitting diode) array and a monitoring module. The control module controls the driving module to drive the LED array to irradiate the photosensitive cells in a culture dish by groups and at regular time; the monitoring module monitors the culture state of the photosensitive cells in the culture dish, and feeds back the culture state to the control module; and the control module adjusts grouping and timing irradiation of the LED array, so as to ensure that the culture state in the culture dish is a set culture state. As the LED array is adopted for irradiating the photosensitive cells, the defect that a size of a whole culture system is too big due to the fact that a size of an irradiation source is too big is avoided. Furthermore, the monitoring module is adopted for monitoring the culture state of various in-vitro cells, so that the in-vitro cells can be cultured. Therefore, the monitoring system for the in-vitro array photostimulation device is small in size, and is suitable for monitoring in-vitro cell culture.

Description

【Technical field】 [0001] The invention relates to the field of optoelectronic technology, in particular to an in vitro cell photostimulation array device monitoring system. 【Background technique】 [0002] The "Optogenetic Technology System" technology (Optogenetic Technology) was first developed in the United States and Germany in the last three years. It is a brand-new biotechnology that integrates genetic engineering, electrophysiology, optics and electronic engineering. This technology is based on the method of gene therapy, using a virus vector to introduce a gene (ChR2) that can encode a light-sensitive cation channel protein cloned from the green alga Chlamydomonas reinhardtii into a specific cell subpopulation for expression. When stimulated by blue light (472nm), Na + The entry of ions into the cell causes the depolarization of the cell membrane and excites the cell. In addition, there is another light-regulated chloride ion pump protein gene NpHR extracted from gr...

AI Technical Summary

Problems solved by technology

However, so far, there is still a lack of a light-stimulating device for "light-gene technology system" for in vitro cultured cells at home and abroad.

At present, although optical fiber devices for various in vivo applications can achieve optical stimulation, the optical fiber stimulation uses a laser as a light source, and a waveform generator is required to adjust the stimulation mode, which is costly and takes up a large space, and cannot be combined with the current Various cell boxes on the market are used

It is only suitable for the photostimulation of a certain group of cells in the local tissue of the animal body, and it cannot be used for the stimulation of cultured cells in vitro

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