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Method for producing neomycin sulfate

A neomycin sulfate and production method technology, applied in fermentation and other directions, can solve the problems of high labor intensity of operators, increased environmental protection pressure, loud noise, etc., and achieve the effect of facilitating material management, reducing material loss, and avoiding loss

Active Publication Date: 2013-05-01
YICHANG SANXIA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the unstable source of broken rice raw materials, the processing technology is relatively simple compared with other raw materials. During the processing process, the labor intensity of the operators is high, the noise is large, there is much dust, and the working environment is harsh.
At the same time, the particle size of rice flour after processing broken rice is relatively coarse, which reduces the degree of absorption and utilization of rice flour by microorganisms on the one hand; on the other hand, too much remaining dry matter brings greater difficulty to clean production and increases environmental protection of enterprises pressure

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A kind of production method of neomycin sulfate, concrete steps are:

[0036] 1) Take the sand from the sand tube and put it into sterile water, shake it well to make a spore suspension; absorb the spore suspension with a straw, apply 0.05ml / piece on the slant medium, and put it in the incubator Cultivate for 4-12 days, and the temperature is controlled at 28±2°C. After the slope is grown, put it in a refrigerator at 2-8°C for storage;

[0037] 2) Put glass beads into the slope obtained in step 1), shake it, knock down the spores on the slope, and then add an appropriate amount of sterile water to wash the spores, pour them into a sterilized glass bottle, and put them in 2-8 Store in refrigerator at ℃ for later use;

[0038] 3) Put the material obtained in step 2) into the sterilized seed tank for cultivation, the temperature is controlled at 35±5°C, the pressure of the tank is 0.02~0.1Mpa, and the cultivation is carried out for 35-45 hours to obtain mycelium;

[0039...

Embodiment 2

[0055] By weight, weigh 4.5 parts of starch, 2.6 parts of peanut cake powder, 0.4 part of soybean cake powder, 1.4 parts of glucose, 0.5 part of yeast powder and 1 part of inorganic salt, put them into the fermentation tank to prepare the fermentation base medium, add water and stir to dissolve Evenly, filter and sterilize, and then put the mycelia grown in step 3) into a fermenter for fermentation and cultivation. The fermentation temperature is 35±5°C, the tank pressure is 0.02~0.1Mpa, and the fermentation time is 130h;

[0056] All the other steps are the same as in Example 1.

Embodiment 3

[0065] By weight, weigh 5.5 parts of starch, 2.8 parts of peanut cake powder, 0.6 part of soybean cake powder, 1.4 parts of glucose, 0.5 part of yeast powder and 1 part of inorganic salt, put them into the fermentation tank to prepare the fermentation base medium, add water and stir to dissolve Evenly, filter and sterilize, and then put the mycelia grown in step 3) into the fermenter for fermentation and cultivation. The fermentation temperature is 35±5°C, the tank pressure is 0.02~0.1Mpa, and the fermentation time is 150h;

[0066] All the other steps are the same as in Example 1.

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PUM

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Abstract

The invention discloses a method for producing neomycin sulfage. The method comprises the steps that a spore suspension liquid is prepared by inoculating and culturing spores, then the spore suspension liquid is subjected to fermentation, absorption, desorption, concentration and refining, and the neomycin sulfate is formed. When a basal culture medium is prepared and fermented, starch is utilized to replace rice flour in the traditional processing in the proportion of 5%; and by means of the measure, the material loss can be reduced, the labor intensity is reduced, the work environment can be improved, and the production can be powerfully guaranteed.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals and relates to a production method of neomycin sulfate. Background technique [0002] In the production process of neomycin sulfate, a large amount of grain processing raw materials are used in the fermentation process, among which, rice flour obtained by crushing and sieving broken rice accounts for the largest proportion in the fermentation medium. Due to the unstable source of broken rice raw materials, the processing technology is relatively simple compared with other raw materials. During the processing process, the labor intensity of the operators is high, the noise is large, there is a lot of dust, and the working environment is harsh. At the same time, the particle size of rice flour after processing broken rice is relatively coarse, which reduces the degree of absorption and utilization of rice flour by microorganisms on the one hand; on the other hand, too much remaining dry matter brin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/52
Inventor 沈联兵何连明周云
Owner YICHANG SANXIA PHARMA
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