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Preparation method of pig lung phospholipid

A technology of porcine lung phospholipids and pig lungs, which is applied in the direction of edible phospholipid compositions, food science, protein food ingredients, etc., can solve the problems of complicated preparation steps and difficult large-scale preparation of porcine lung phospholipids, and achieve the effect of increasing the added value of utilization

Inactive Publication Date: 2013-05-08
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

The preparation steps of these two methods are complicated, and it is difficult to prepare porcine lung phospholipids on a large scale.

Method used

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  • Preparation method of pig lung phospholipid

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Effect test

Embodiment 1

[0018] Add 5,000 grams of freshly washed pig lungs to 15,000 ml of deionized water for beating, pass through a 100-mesh sieve, heat to 35°C, add 250 grams of 1398 protease to react for 2 hours, cool to room temperature, centrifuge to remove slag, and enzymolysis solution Add 20000 milliliters of n-hexane for extraction, separate and obtain the n-hexane layer, desolvate, wash the desolvated matter with acetone until there is no oil, and obtain 10.5 grams of porcine lung phospholipids with a phospholipid content of 91.5%.

Embodiment 2

[0020] Add 5,000 grams of freshly washed pig lungs to 50,000 ml of deionized water for beating, pass through a 100-mesh sieve, heat to 35°C, add 250 grams of 1398 protease to react for 5 hours, cool to room temperature, centrifuge to remove slag, and enzymolysis solution Add 100,000 milliliters of n-hexane for extraction, separate and obtain the n-hexane layer, desolvate, wash the desolvated matter with acetone until there is no oil, and obtain 8.3 g of porcine lung phospholipids with a phospholipid content of 93.1%.

Embodiment 3

[0022] Add 5,000 grams of freshly washed pig lungs to 15,000 ml of deionized water for beating, pass through a 100-mesh sieve, heat to 35°C, add 250 grams of 1398 protease to react for 5 hours, cool to room temperature, centrifuge to remove slag, and enzymatic hydrolysis solution Add 30,000 milliliters of n-hexane for extraction, separate and obtain the n-hexane layer, desolvate, wash the desolvated matter with acetone until there is no oil, and obtain 12.1 g of porcine lung phospholipids with a phospholipid content of 90.7%.

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Abstract

Belonging to the field of pig lung processing, the invention relates to a preparation method of pig lung phospholipid. The method comprises: subjecting a pig lung and water to beating, adding protease to digest the slurry, conducting centrifugation, using a solvent to extract lipid, desolventizing the extracted lipid, and carrying out deoiling with acetone, thus obtaining the pig lung phospholipid with a phospholipid content greater than 90%.

Description

technical field [0001] The invention discloses a preparation method of pig lung phospholipid, belonging to the technical field of pig lung processing. Background technique [0002] Porcine lung phospholipids can effectively reduce the surface tension of alveoli, and have many important functions such as preventing atelectasis and pulmonary edema. Certain curative effect. Porcine lung phospholipid is one of the main lipid components of porcine lung alveoli, and its content in porcine lung tissue reaches 0.4%-0.8%. About 70% of porcine lung phospholipid is phosphatidylcholine, and dipalmitoylphosphatidylcholine Mainly. [0003] There are currently two main methods for the preparation of pig lung phospholipids. One is the method of perfusion with normal saline. The pig lungs are washed and extracted by perfusion with normal saline, and the crude product is obtained by differential gradient centrifugation. extraction, rotary evaporation and drying. Another method is the tiss...

Claims

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Application Information

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IPC IPC(8): C07F9/10
Inventor 史苏佳曹栋曹沛
Owner JIANGNAN UNIV
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