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Method for rapidly recovering punicalagin from pomegranate rind extraction waste based on solvent effect

A technology of punicalagin and pomegranate peel, which is applied in a field of rapid recovery of punicalagin, which can solve the problems of high difficulty, low punicalagin, poor water solubility and dispersibility, etc.

Active Publication Date: 2021-09-14
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the poor water solubility and dispersibility of the precipitate, and the content of punicalagin is lower than that of the pomegranate peel extract, it is difficult to quickly enrich the punicalagin in the precipitate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] a. Take the pomegranate peel and crush it into a coarse powder, add 10 times the concentration of 40% ethanol, extract once at a temperature of 71 ° C, filter for 3 hours, combine the filtrate to recover ethanol under reduced pressure, and concentrate to the equivalent of crude drug per mL 0.15-0.2g concentrated solution; add the concentrated solution to the treated HPD300 macroporous adsorption resin column, absorb for 3 hours, first wash with deionized water until the color of the effluent is light, and then use 2 times the column volume 60% ethanol was eluted, the alcohol-containing eluate was collected, the ethanol was recovered, concentrated under reduced pressure and vacuum-dried, pulverized, and passed through a 80-mesh sieve to obtain the pomegranate peel polyphenol extract;

[0017] b. Weigh 1096g of pomegranate peel extract, dissolve it in 8L of water, and ultrasonically dissolve it. Put it in a refrigerator at a temperature of 4°C for 10 hours, pour out the su...

Embodiment 2

[0021] a. Take the pomegranate peel and crush it into a coarse powder, add 15 times the concentration of 40% ethanol, extract twice at a temperature of 75°C for 1 hour each time, filter, combine the filtrate to recover ethanol under reduced pressure, and concentrate to the equivalent of Concentrate of 0.15-0.2g of crude drug; add the concentrate to the treated HPD300 macroporous adsorption resin column, absorb for 5 hours, first wash with deionized water until the color of the effluent is light, and then use 3 times eluting with 60% ethanol by column volume, collecting the eluent containing alcohol, recovering ethanol, concentrating under reduced pressure and vacuum drying, pulverizing, passing through a 100-mesh sieve to obtain the pomegranate peel polyphenol extract;

[0022] b. Weigh 1096g of pomegranate peel polyphenol extract, dissolve it in 8L of water, and ultrasonically dissolve it. Put it in a refrigerator at a temperature of 4°C for 10 hours, pour out the supernatant,...

Embodiment 3

[0026]a. Take the pomegranate peel and crush it into a coarse powder, add 18 times the concentration of 40% ethanol, extract 3 times at a temperature of 78°C, each time for 1 hour, filter, combine the filtrate to recover ethanol under reduced pressure, and concentrate to the equivalent of Concentrate of 0.15-0.2g of crude drug; add the concentrate to the treated HPD300 macroporous adsorption resin column, absorb for 8 hours, first wash with deionized water until the color of the effluent is light, and then use 4 times eluting with 60% ethanol in the column volume, collecting the eluent containing alcohol, recovering ethanol, concentrating under reduced pressure, drying in vacuum, pulverizing, and passing through a 80-120 mesh sieve to obtain the pomegranate peel polyphenol extract;

[0027] b. Weigh 1096g of pomegranate peel extract, dissolve it in 8L of water, and ultrasonically dissolve it. Put it in a refrigerator at a temperature of 4°C for 10 hours, pour out the supernatan...

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PUM

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Abstract

The invention relates to a method for rapidly recovering punicalagin from pomegranate rind extraction waste based on a solvent effect. According to the method, based on a solvent effect, methanol water, acetonitrile water or formic acid water is used as a dispersing solvent to disperse a precipitate (namely pomegranate rind extraction waste) generated in a precipitation pretreatment process of a pomegranate ring extract, and then a sample is injected to a reversed-phase chromatographic column for separation; and a solvent effect is formed in the separation process by controlling the volume and the elution strength of a sample injection solvent and the elution strength of a mobile phase. Large-polarity punicalagin is rapidly eluted at a solvent peak, and small-polarity impurities are retained on a reversed-phase chromatographic column, so that a separation effect is achieved. According to the method, the pomegranate rind waste with the punicalagin content of 12% can be rapidly purified into a punicalagin-rich component with the punicalagin content range of 18-40%. The method provided by the invention is simple and rapid, has high purification efficiency, and provides powerful support for recycling the pomegranate rind extraction waste obtained by precipitation pretreatment in the pomegranate rind extract.

Description

technical field [0001] The present invention relates to a method for quickly recovering punicalagin from pomegranate peel waste extraction waste based on solvent effect. Based on the characteristics of solvent effect in liquid chromatography, the method can realize rapid separation of compounds with large polarity differences. And it is used to rapidly enrich punicalagin in the waste extracted from pomegranate peel, thus providing a strong support for the high-value comprehensive utilization of pomegranate peel. Background technique [0002] Liquid chromatography is a high-efficiency separation method developed in modern times, and has been widely used in biopharmaceutical and other fields. Solvent effects are a common negative problem in the use of liquid chromatography. During the chromatographic separation process, when the elution strength of the injection solvent is higher than that of the mobile phase, some samples may not be effectively retained on the chromatographi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/74
CPCG01N30/02G01N30/06G01N30/74G01N2030/062G01N2030/065
Inventor 阿吉艾克拜尔·艾萨孙光映
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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