Efficient limestone eroded bacillus megaterium NL-7 and application thereof
A technology of Bacillus megaterium and NL-7, which is applied in the field of microorganisms to achieve good application development prospects, reduce bulk density, and accelerate erosion into soil
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Embodiment 1
[0018] The acquisition and identification of embodiment 1 bacterial strain:
[0019] Bacterial colonies on the exposed rock surface of limestone (Mufu Mountain, Nanjing City, Jiangsu Province) were collected (see figure 1 ), using the dilution plate method, in a 28°C incubator, to screen the medium (5g sucrose, 2g Na 2 HPO 4 , 0.5g MgSO 4 ·7H 2 O, 0.005g FeCl 3 , 0.1g CaCO 3 , 1g limestone powder (cleaned Shogunate rock, passed through a 200-mesh sieve, see Table 1 for the main relevant chemistry), 18g agar powder, 1000mL distilled water) for about 48 hours, picked a single colony, repeatedly streaked and purified, and the obtained strain They were respectively inoculated on the slant of beef extract peptone solid medium, and stored in a refrigerator at 4°C to obtain pure strains. The main biological characteristics of the strain: cultured on beef extract peptone solid medium at 28°C, the bacterium is rod-shaped, the end is round, Gram staining is positive, there are sp...
Embodiment 2
[0023] Limestone erosion ability of embodiment 2 bacterial strains:
[0024] Use an inoculation loop to inoculate a small amount of pure strains into a 250mL Erlenmeyer flask filled with 50mL screening medium (the medium is the same as above, without adding agar powder), culture at 28°C and shake at 180r / min for 72h to obtain activated strains.
[0025] Take 2mL of the above bacterial solution and inoculate it in 50mL fermentation medium (15g sucrose, 0.2g MgSO 4 ·7H 2 O, 0.1g CaSO 4 2H 2 O, 0.2g NaCl, 0.2g yeast extract, 0.5g (NH 4 ) 2 SO 4 , 10g limestone powder, 1000mL distilled water, pH 7.0~7.5) in a 250mL Erlenmeyer flask, 25°C, 180r / min shaking culture.
[0026] On the 15th day of fermentation culture, pour the bacterial suspension into a sterilized centrifuge tube, centrifuge at 5000r / min at 4°C for 10min, draw the supernatant with a sterile syringe, and filter it through a bacterial filter (the pore size of the filter membrane is 0.22um). A sterile filtrate was...
Embodiment 3
[0033] The influence of embodiment 3 bacterial strains on spraying substrate:
[0034] After activating the strain (the method is the same as in Example 2), mix it into the spraying matrix (woodland soil, add humic acid organic fertilizer 25%, limestone powder (through 100 mesh sieve) 4%, spraying wood fiber 0.3% at 100mL / kg , water-retaining agent 0.3%, greening spraying adhesive 0.03%), the control was mixed with the same volume of medium in the spraying matrix, and the pot test was carried out (pot diameter 17cm, depth 15cm), placed in a greenhouse at 25°C Keep the spraying substrate moist. After 4 months, the bulk density of the sprayed substrate was tested by the ring knife method; the impact resistance of the sprayed substrate was measured by the CC Sobowski anti-shock tester; the shear resistance of the sprayed substrate was measured by the soil shear tester.
[0035] Soil bulk density reflects the comprehensive physical conditions of the soil such as texture, structure ...
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