Kit for detecting glucose by using glucose dehydrogenase method and preparation method

Abstract

The invention discloses a kit for detecting glucose by using a glucose dehydrogenase method, and belongs to the kit for detecting glucose containing enzymes. The kit disclosed by the invention comprises 4 bottles of glucose dehydrogenase method reagents and a glucose standard solution. The kit disclosed by the invention comprises 8.0mmol / L of sodium oxalate dissolved in the phosphate buffer solution of which the concentration is 120.0mmol / L, 6000U / L of glucose dehydrogenase, 180U / L of mutarotase, 4.0 mmol / L of NAD + (Nicotinamide Adenine Dinucleotide), and 0.2% of ProClin300 preservative. In the determination, the sodium oxalate inhibits activities of endogenous lactate dehydrogenase and alpha-hydroxybutyrate dehydrogenase to control interfere reaction, so as to increase the specificity of the reaction, and improve the accuracy of detection results. The kit disclosed by the invention is less affected by a sample / reagent volume ratio during a detecting process, and the linear range is up to 35.0mmol / L. The use method of the kit is identical with the original glucose dehydrogenase method, so that the kit does not increase the burden on an experimenter, and almost does not increase the cost of the reagent, is economical, convenient and easy and is the kit for determining glucose by using glucose dehydrogenase method with high accuracy.

Description

technical field [0001] The invention belongs to an enzyme-containing glucose detection kit, in particular to a glucose dehydrogenase detection kit and a preparation method. Background technique [0002] There are as many as 12 known determination methods for glucose. Among these methods, some methods are susceptible to high or low deviation due to the interference of reducing substances and other sugars in the reaction solution and are rarely used. At present, the most commonly used method for detecting glucose in clinical laboratories at home and abroad is the enzymatic method. Using enzymes as reagents can improve the specificity. The glucose oxidase method is easily interfered by vitamin C, uric acid, urea, bilirubin, and creatinine. The substance does not interfere with the glucose dehydrogenase method. At present, the glucose dehydrogenase method kits used in various laboratories have different manufacturers, but the composition of the kits is basically similar. The bu...

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Problems solved by technology

[0015] In order to solve the problem of endogenous interference in the determination of glucose by the glucose dehydrogenase method in the prior art, the present invention provides a test kit for resisting endogenous interference and improving the accuracy of glucose determination by the glucose dehydrogenase method in clinical laboratories and Preparation

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