F0F1-ATPase rotary molecular motor sensor kit for detecting Vibrio cholera

Abstract

An F0F1-ATPase rotary molecular motor sensor kit for detecting Vibrio cholera belongs to the field of the rapid detection of food microbes, and mainly solves an overlong time (about 5 days) problem of traditional detection methods. The kit is mainly characterized in that an F0F1-ATPase rotary molecular motor sensor on a chromatophore is utilized, an F0F1-ATP synthase can rapidly rotate, and the coupling between a catalytic site and proton transfer is established through rotating the F0F1-ATP synthase. An ompW probe is connected to the epsilon subunit of the ATP synthase, a sample to be measured and a negative contrast substance are respectively combined with a biosensor, the ATP outputs 20min after the ATP catalytic synthesis are compared, the ATP synthetic outputs can be measured through the amount of H<+> in the environment, and the H<+> amount can be obtained through the fluorescence intensity of H-DHPE. The kit can realize the rapid, sensitive and high-flux detection of the Vibrio cholera in the sample to be measured.

Description

technical field [0001] The invention relates to a rapid detection technology for food microorganisms. Background technique [0002] The traditional Vibrio cholerae detection method requires steps such as non-selective enrichment, selective enrichment, separation, screening and identification for each test item. It usually takes 4 days to issue a test report, which seriously affects the quality and shelf life of the goods. . Some new technologies such as PCR technology, immunological detection technology, biochip technology and other detection methods still rely on traditional detection steps, that is, they need to be enriched, which is time-consuming and labor-intensive. It usually takes several days to complete a detection. This not only increases the workload of the laboratory, but also is not conducive to the monitoring of the production process in the food industry, the quality control of the final product, and the management and control of food safety by government dep...

AI Technical Summary

Problems solved by technology

[0002] The traditional Vibrio cholerae detection method requires steps such as non-selective enrichment, selective enrichment, separation, screening and identification for each test item. It usually takes 4 days to issue a test report, which seriously affects the quality and shelf life of the goods.

Some new technologies such as PCR technology, immunological detection technology, biochip technology and other detection methods stil

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