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Method for extracting perillaseed hydrophilic chemical component from perillaseed oil residue

A chemical composition, hydrophilic technology, applied in the directions of medical preparations containing active ingredients, non-central analgesics, plant/algae/fungus/moss ingredients, etc. effect of value

Active Publication Date: 2013-05-15
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Research on the medicinal value of perilla oil residue is still blank at home and abroad

Method used

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  • Method for extracting perillaseed hydrophilic chemical component from perillaseed oil residue
  • Method for extracting perillaseed hydrophilic chemical component from perillaseed oil residue

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1, extract rosmarinic acid from perilla oil residue

[0027] Take perilla oil residue 2kg, dry at 40-50°C, use ethanol aqueous solution with a volume fraction of 20% as the extractant, and soak in a constant temperature water bath at 80°C according to the material-to-solvent ratio of 1:30 (kg / L, w / v). Extract 2 times, 1h each time, and combine the extracts. Use 18% hydrochloric acid solution to adjust the pH value of the extract between 2.0-2.5, add ethyl acetate to extract 3 times according to the ratio of solid to liquid to solvent 5:3 (v / v), combine the extracts, and concentrate under reduced pressure , that is, the total extract of the hydrophilic components of perilla oil residue. Methanol was settled to 50mL, and the rosmarinic acid yield of 0.52% was measured by high performance liquid chromatography (the yield was calculated according to "the amount of rosmarinic acid / the quality of perilla oil residue", and the content determination method was with ...

Embodiment 2

[0028] Embodiment 2, extract rosmarinic acid from perilla oil residue

[0029] Get perilla oil residue 0.5kg, 40-50 ℃ of oven dry, be the mixed solvent that the volume ratio of 95% ethanol aqueous solution and alkali water (the borax aqueous solution of mass content 0.2%) volume ratio is 8: 2 is extraction agent, according to The ratio of material to solvent was 1:6 (kg / L, w / v), heated to reflux for 2 hours, filtered, and the filter residue was further refluxed and extracted with the above extractant for 3 times, each time for 1 hour, and the filtrates were combined and concentrated under reduced pressure. Use hydrochloric acid with a mass fraction of 18% to adjust the pH value of the concentrated solution to 4.5-5.0, then extract once with an equal volume of petroleum ether, discard the petroleum ether part; continue to adjust the pH value of the remaining solution to 3.0-3.5, and so on Extract 2 times with volumetric ethyl acetate, collect the ethyl acetate extract; adjust t...

Embodiment 3

[0030] Embodiment 3, extract rosmarinic acid from wild perilla seed (adulterated product, containing perilla seed) oil residue

[0031] Take 0.5kg of perilla oil residue, dry at 40-50°C, use a mixture of water-ethanol-1M hydrochloric acid (20:80:1, v / v / v) as the extractant, and use a material-to-solvent ratio of 1:30 ( kg / L, w / v), cold soaked overnight. Filter and collect the filtrate. Add the above extractant to the filter residue and continue to heat and reflux for extraction for 2 hours. The material-to-solvent ratio is 1:6 (kg / L, w / v). The combined filtrates were concentrated under reduced pressure. Extract once with an equal volume of petroleum ether, and discard the petroleum ether part. Then extract twice with ethyl acetate and n-butanol respectively, and combine the extracts. Concentrate under reduced pressure to obtain the total extract of the hydrophilic components of perilla oil residue. Methanol was fixed to 25mL, and the yield of rosmarinic acid was measured ...

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Abstract

The invention discloses a method for extracting perillaseed hydrophilic chemical components from perillaseed oil residue. The method employs perillaseed oil residue as a raw material to extract hydrophilic chemical components in the perillaseed. The method adopts specific methods including solvent extraction, organic solvent extraction and resin separation and purification. The obtained hydrophilic chemical components mainly comprise flavone, flavone glycosides, phenylpropionic acid, phenpropionate and glycosides such as rosmarinic acid and rosmarinic acid glycoside. Calculated by rosmarinic acid as a representative component, yield of the representative component in the perillaseed oil residue is higher or close to yield of the component in perillaseed; therefore, perillaseed oil residue can be used as a substitute for raw material for preparation of perillaseed hydrophilic component.

Description

technical field [0001] The invention relates to a method for extracting the hydrophilic chemical components of perilla from perilla oil residue. Background technique [0002] Perilla is Perilla frutescens (L.) Britt. [Perilla frutescens (L.) Britt.var.arguta (Benth.) Hand.-Mazz.], Perilla frutescens (L.) Britt. [Ocimum frutescens L.; Perilla frutescens (L.) Britt.var.typica Makino] and wild perilla P.frutescens (L.) Britt.var.purpurascens (Hayata) H.W.Li, Perilla frutescens (L.) Britt .var.crispa(Thunb.)Hand.-Mazz. The dry and mature fruit is a well-known resource for both medicine and food. Its α-linolenic acid content is the highest among the vegetable oils found so far, and it has extremely high Economic value and health function. Because the oil residue after oil extraction of perilla is often discarded or directly used as feed, which reduces the economic value of perilla, it is of great significance to research and develop the medicinal value of perilla oil residue. ...

Claims

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Application Information

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IPC IPC(8): A61K36/535A61K8/97A61K8/37A61K8/60A61K8/49A61P9/10A61P3/06A61P39/06A61P1/16A61P31/04A61P29/00A61P35/00A61P37/02A23L1/30A61K131/00
Inventor 陈爱亮管政
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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