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Method for preparing nano microcrystalline cellulose by hydrolyzing phyllostachys edulis fiber with cellulase

A technology of cellulase and moso bamboo fiber, which is applied in the field of pulp and papermaking engineering, can solve the problems of high price, long growth cycle, insufficient supply of fiber raw materials, etc., and achieve the alleviation of environmental pollution, expansion of the range of raw materials, and important economic and social benefits Effect

Inactive Publication Date: 2015-05-20
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to overcome the problems of insufficient supply of fiber raw materials for the preparation of nano-microcrystalline cellulose, high price, and long growth cycle, resource utilization of my country's rich moso bamboo resources, and low-pollution enzymatic hydrolysis to prepare high-value-added renewable nano-new materials, the present invention The purpose is to provide a method for preparing nano-microcrystalline cellulose by hydrolyzing moso bamboo fiber with cellulase

Method used

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  • Method for preparing nano microcrystalline cellulose by hydrolyzing phyllostachys edulis fiber with cellulase
  • Method for preparing nano microcrystalline cellulose by hydrolyzing phyllostachys edulis fiber with cellulase

Examples

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Embodiment 1

[0021] 1) Deleafing, washing, and drying the moso bamboo, crushing it with a plant grinder, and filtering it through a 60-mesh sieve to obtain moso bamboo powder with a particle size less than or equal to 0.25 mm;

[0022] 2) The moso bamboo powder obtained in step 1) was reacted in 8.0wt% KOH solution at 85°C for 120min under mechanical stirring, the mass ratio of moso bamboo powder to KOH solution was 1:15, repeated twice, and washed with distilled water Net residual alkali, obtain moso bamboo fiber;

[0023] 3) Using 5.0wt% NaClO solution under the action of mechanical stirring, the bamboo fiber obtained in step 2) was bleached at 70°C for 90 minutes. The mass ratio of moso bamboo fiber to NaClO solution was 1:10, repeated 4 times, and washed with distilled water Residual chlorine to obtain high-purity moso bamboo fiber;

[0024] 4) The high-purity moso bamboo fiber obtained in step 3) was placed in a solution of extracellular cellulase produced by Trichoderma reesei at pH...

Embodiment 2

[0027] 1) Deleafing, washing, and drying the moso bamboo, crushing it with a plant grinder, and filtering it through a 60-mesh sieve to obtain moso bamboo powder with a particle size less than or equal to 0.25 mm;

[0028] 2) React the moso bamboo powder obtained in step 1) in a 7.0wt% KOH solution at 90°C for 150 minutes under mechanical stirring, the mass ratio of the moso bamboo powder to the KOH solution is 1:12, repeat twice, and wash with distilled water Net residual alkali, obtain moso bamboo fiber;

[0029] 3) Using 4.0wt% NaClO solution under the action of mechanical stirring, the moso bamboo fibers obtained in step 2) were bleached at 80°C for 120 minutes, the mass ratio of moso bamboo fibers to NaClO solution was 1:8, repeated 4 times, and washed with distilled water Residual chlorine to obtain high-purity moso bamboo fiber;

[0030]4) The high-purity moso bamboo fiber obtained in step 3) was placed in a solution of extracellular cellulase produced by Trichoderma r...

Embodiment 3

[0033] 1) Deleafing, washing, and drying the moso bamboo, crushing it with a plant grinder, and filtering it through a 60-mesh sieve to obtain moso bamboo powder with a particle size less than or equal to 0.25 mm;

[0034] 2) React the moso bamboo powder obtained in step 1) in a 6.0wt% KOH solution at 95°C for 180 min under mechanical stirring, the mass ratio of moso bamboo powder to KOH solution is 1:10, repeat twice, and wash with distilled water Net residual alkali, obtain moso bamboo fiber;

[0035] 3) Using 3.0wt% NaClO solution under the action of mechanical stirring, the bamboo fiber obtained in step 2) was bleached at 90°C for 150 minutes. The mass ratio of moso bamboo fiber to NaClO solution was 1:5, repeated 4 times, and washed with distilled water Residual chlorine to obtain high-purity moso bamboo fiber;

[0036] 4) The high-purity moso bamboo fiber obtained in step 3) was placed in a solution of extracellular cellulase produced by Trichoderma reesei at pH 3, 70°C...

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Abstract

The invention discloses a method for preparing nano microcrystalline cellulose by hydrolyzing phyllostachys edulis fiber with cellulase. The key point of the method is that the phyllostachys edulis is treated by an alkaline and chloride-containing solution to purity cellulose, the phyllostachys edulis fiber is hydrolyzed by use of extracellular cellulase generated by trichoderma reesei, and the phyllostachys edulis nano microcrystalline cellulose product is collected by an ultrafiltration membrane. The reaction conditions are moderate, the preparation process is environment-friendly, and the method is particularly applicable to the low-pollution preparation of phyllostachys edulis nano microcrystalline cellulose. The method disclosed by the invention introduces phyllostachys edulis resource into the preparation of the fiber material of nano microcrystalline cellulose, expands the range of raw material for preparing nano microcrystalline cellulose, and is favorable for accelerating the breakthrough of the limitation on the source of the raw material of nano microcrystalline cellulose; and meanwhile, the method hydrolyzes phyllostachys edulis fiber by use of cellulase instead of a chemical reagent, relieves the problem of environmental pollution caused in the traditional preparation process of nano microcrystalline cellulose, and has important economic and social benefits.

Description

technical field [0001] The invention relates to a method for preparing nano-microcrystalline cellulose, in particular to a method for preparing nano-microcrystalline cellulose by hydrolyzing moso bamboo fiber with cellulase, and belongs to the field of pulp and papermaking engineering. Background technique [0002] Cellulose molecules in natural fibers are linear chains composed of β-(1,4)-D-glucose groups, composed of nematically ordered crystalline regions and disordered amorphous regions, relying on hydrogen inside and outside the molecule Bonds and van der Waals forces maintain the self-assembled supramolecular structure and fibril morphology. Nano-microcrystalline cellulose is a solid product with a limit degree of polymerization with nanoscale and excellent performance obtained from natural cellulose through microcrystallization. Nanocrystalline cellulose not only has the basic structure and properties of ordinary cellulose, but also has the characteristics of nanopar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B15/08
Inventor 张勇姚菊明马兰曹建军庄源
Owner ZHEJIANG SCI-TECH UNIV
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