Preparation method for rhodiola rosea ferment by using red ginseng and composition containing same and capable of improving fatigue and increasing exercise capacity
A manufacturing method and technology of Rhodiola rosea, applied in the direction of drug combination, medical preparations containing active ingredients, functions of food ingredients, etc., can solve the problems that Rhodiola rosea cannot be fermented, and there is no record of Rhodiola rosea fermented products, etc., to achieve Excellent anti-fatigue, high content effect
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manufacture example 1
[0044] Production example 1: Production of fermented product of Rhodiola rosea alone
[0045] Armor: material
[0046] The main raw material Rhodiola rosea (Rhodiola rosea, figure 1 ) was purchased from China in 2010, after micro-grinding, a pulverizer (Cyclotec TM 1093, FOSS Co., Denmark) was used for powdering. Red ginseng uses commercially available red ginseng powder (NH Korean ginseng, 6-year-old root).
[0047] The strains used in this experiment utilized 10 species of lactic acid bacteria belonging to the genus Lactobacillus, Pediococcus and Bifidobacterium deposited in Korea Food Research Institute shown in Table 1. The strains were suspended in sterile 50% glycerol and stored in a deep freezer at -70°C for a long time.
[0048] Table 1
[0049] differentiate
strain name
medium used
L1
Lactobacillus acidophilus 128
MRS
L2
Lactobacillus plantarum 144
MRS
L3
MRS
...
experiment example 1
[0055] Experimental Example 1: Confirmation of Fermentation Properties of Rhodiola Solo Fermentation Product
[0056] In order to confirm the fermentation characteristics of the Rhodiola rosea extract-only fermented product of Production Example 1, changes in the pH value and the number of viable cells of the fermented product at 24 hours and 48 hours after inoculation were confirmed.
[0057] The number of viable bacteria is calculated as follows: Dilute 1 m of the sample with 9 ml of sterilized physiological saline and mix it thoroughly, then dilute it step by step, take 0.1 ml, smear it on MRS agar, double Smear the genus Bifidobacterium on BL agar containing 5% horse blood, store it in an anaerobic state as much as possible in an anaerobic culture tank (BBL Gas Pak anaerobic system), culture it at 37°C for 24 to 48 hours, and measure The number of colonies generated was calculated by multiplying the average number of colonies by the dilution factor to calculate the number ...
manufacture example 2
[0078] Production example 2: Production of Rhodiola rosea and red ginseng fermented product
[0079] A. Manufacture of mixed extract of rhodiola rosea and red ginseng
[0080] The solid content of the production example 1 is the red ginseng extract with a solid content of 0.5 wt% of the Rhodiola rosea extract of 0.5% by weight and the same method as the production method of the rhodiola rosea extract of Production example 1, The mixed extracts of Rhodiola rosea and red ginseng were mixed according to the mixing ratio in Table 4 below.
[0081] Table 4
[0082] differentiate
Rhodiola rosea: red ginseng (by weight)
2-1
100:0
2-2
90:10
2-3
80:20
2-4
70:30
2-5
60:40
2-6
50:50
[0083] B. Manufacture of Rhodiola rosea and red ginseng mixed fermented product
[0084] Using L.acidophilus 128 (L.acidophilus 128) and Bifidobacterium longum (B.longum), which grow relatively w...
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