Pine needle extract for preventing phytopathogen
A technology of plant pathogenic bacteria and pine needle extract, applied in the field of biopesticides against plant pathogenic bacteria, can solve the problems of lack of research on the activity of anti-plant pathogenic bacteria, no bactericidal spectrum of plant pathogenic bacteria, etc., achieve low production cost, easy storage, and method The effect of simple process
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Embodiment 1
[0035] In the preparation process of pine needle-derived biopesticides, the collected pine needles were cleaned of impurities, dried in a 50°C electric constant temperature oven, crushed to 40 mesh, and the obtained powder was stored at room temperature for later use. Weigh a certain amount of pine needle powder, add 15 times the volume of 40-70% ethanol solution, reflux in a water bath at 60-90°C for 1 hour, then use a circulating vacuum pump to filter, collect the filtrate, and add 15 times the volume of 40- 70% ethanol solution, for the second extraction, reflux in a water bath for 1 hour and suction filtration, collect the filtrate, extract 3 times in the same way, then combine the filtrate, evaporate the solution with a rotary evaporator to obtain a paste, put it in a vacuum Dry in a drying oven at 50°C to constant weight, take out and grind to obtain the extract powder, which is the pine needle-derived biopesticide, and it can be stored at room temperature.
Embodiment 2
[0037] For the determination of plant pathogenic bacteria resistance, use the production rate method to measure the biological resistance of plant pathogenic bacteria to the pine needle-derived biological pesticide prepared in (1). The specific operation is as follows: take the pine needle-derived biological pesticide prepared in (1) and prepare it with water 20-100mg / ml medicinal solution; preparation of medicinal medium: take 0.5ml of the prepared medicinal solution in a sterilized 10ml test tube, add 9.5ml of PDA medium at about 50°C, shake well, and pour it into a sterilized petri dish Serve. Bacteria culture: select the colony circle in the same growth potential, use a sterile spatula to scoop out the hole puncher to make a cake, and dig out the culture medium in the hole, and place it upside down on the plate of the above-mentioned drug-dosed medium, three times per plate. indivual. 28 ℃ constant temperature inverted culture for 43h. The diameter of the colony circle w...
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