Method for extracting liquorice flavonoids from liquorice residue

A technology of licorice flavonoids and licorice residue is applied in the directions of pharmaceutical formulations, medical preparations containing active ingredients, food science, etc., and achieves the effects of cost safety, non-toxicity, improved yield, and simple process operation.

Active Publication Date: 2013-06-12
惠州市九惠药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned modern technology of enzymatic hydrolysis and ultrasonic wall breaking has been introduced into the technical scheme of extracting total plant flavonoids, but there are still some deficiencies that need to be improved

Method used

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  • Method for extracting liquorice flavonoids from liquorice residue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Extract total flavonoids from licorice

[0025] (1) Do the preparatory work first, that is, dry the licorice residue and crush it into a coarse powder; take an equal amount of cellulase and pectinase and add water to prepare a compound enzyme solution with a concentration of 0.1%; take chitosan 0.8g, added to 80mL concentration of 1% acetic acid solution to make a chitosan colloid solution with a chitosan concentration of 1%, and set aside; take 4g of 101 fruit juice clarifier, add it to 80mL distilled water to make a concentration of 101 fruit juice clarifier It is 5% aqueous solution of 101 fruit juice clarifier, and it is set aside.

[0026] (2) Take 1000g of licorice residue coarse powder and add it to the prepared compound enzyme solution. ) after 1.0 h of treatment, the temperature was raised rapidly to 90°C, kept for 10 min to inactivate the enzyme, filtered, and the filtrate was concentrated under reduced pressure to a relative density of 1.05 (60°C) to obta...

Embodiment 2

[0035](1) Dry the licorice residue and crush it into a coarse powder; take an equal amount of cellulase and pectinase and add water to prepare a compound enzyme solution with a concentration of 0.15%; prepare a chitosan concentration of 1% chitosan colloid solution and 101 fruit juice clarifying agent concentration are 5% 101 fruit juice clarifying agent aqueous solution, standby.

[0036] (2) Take 1000g of licorice residue coarse powder, add it to the prepared compound enzyme solution, the amount added is 1g licorice residue / 25ml compound enzyme solution, then adjust the pH value of the solution to 4.5, and then ultrasonicate at 40°C (50KHZ, 160W ) after 1.0 h of treatment, the temperature was raised rapidly to 90°C, kept for 10 min to inactivate the enzyme, filtered, and the filtrate was concentrated under reduced pressure to a relative density of 1.05 (60°C) to obtain a concentrate.

[0037] (3) At 60°C, add 80mL of chitosan colloid solution to the 1000ml concentrated solut...

Embodiment 3

[0041] (1) Dry the licorice residue and crush it into a coarse powder; take an equal amount of cellulase and pectinase and add water to prepare a compound enzyme solution with a concentration of 0.05%; prepare the chitosan concentration by the same method as in Example 1 1% chitosan colloid solution and 101 fruit juice clarifying agent concentration are 5% 101 fruit juice clarifying agent aqueous solution, standby.

[0042] (2) Take 1000g of licorice residue coarse powder and add it to the prepared compound enzyme solution. ) after 1.5 hours of treatment, the temperature was rapidly raised to 90°C, kept for 10 minutes to inactivate the enzyme, filtered, and the filtrate was concentrated under reduced pressure to a relative density of 1.05 (60°C) to obtain a concentrate.

[0043] (3) At 60°C, add 80mL of chitosan colloid solution to the 1000ml concentrated solution obtained in the previous step, stir well, then add 80mL of 101 juice clarifier aqueous solution, stir well, let st...

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Abstract

The invention relates to a method for extracting liquorice flavonoids, and in particular relates to a method for extracting liquorice flavonoids from liquorice residue. The method comprises the following steps of: (1) ultrasonic enzymolysis: drying and grinding the liquorice residue, and performing ultrasonic treatment on a mixed solution of cellulase and pectinase; (2) flocculation purification: adding a chitosan colloidal solution and an aqueous solution of 101 fruit juice clarifying agent into a concentrated solution; and (3) passing through a macroporous resin column: passing the supernate through a D101 resin column, eluting with ethanol with the volume concentration of 50%, concentrating the elution liquid, and drying under reduced pressure to obtain liquorice total flavonoids. The method provided by the invention can be used for obviously improving the extraction rate and purity of liquorice total flavonoids.

Description

Technical field: [0001] The present invention involves a method of extracting licorice flavonoids, especially the method of extracting licorice flavonoids from licorice residue. Background technique: [0002] Licorice is the dry roots and rhizomes of Glycyrrhiza Inflata Bat or Glycyrrhiza Glabra LIt is rich in components of triangles and flavonoids, where the five -ring triangular compound licoric acid is one of the main active ingredients.Pharmaceutical manufacturers often use water lifting, water -containing alcohol, alkali and other methods to extract licorice from licorice medicinal materials. After extraction, the remaining licorice drugs are usually abandoned as industrial waste.Further research shows that a large amount of flavonoids are rich in flavonoids, which have extensive biological activity, such as bacteriostatic, antifungal, enhanced the body's immune function, anti -tumor, anti -ulcer, antioxidant, antioxidant, Preservation of liver and lowering blood sugar.Ther...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/484A61K8/97A23L1/29A23L33/00
Inventor 黄辉球刘强江洁
Owner 惠州市九惠药业有限公司
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