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Cerebroprotein hydrolysate and lyophilized powder thereof for injection

A technology of cerebroprotein hydrolyzate and freeze-dried powder injection, which is applied in the field of medicine, can solve the problems of affecting the drug safety of patients, restricting popularization and application, and poor homogeneity, so as to achieve excellent therapeutic effects, reduce production costs, and increase yields. Effect

Active Publication Date: 2013-06-12
SHANXI PUDE PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the above-mentioned technical solutions can obtain a cerebroprotein hydrolyzate and its preparation, there are generally defects of low extraction rate and poor homogeneity, which indirectly affects the drug safety of patients and limits its popularization and application.

Method used

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  • Cerebroprotein hydrolysate and lyophilized powder thereof for injection
  • Cerebroprotein hydrolysate and lyophilized powder thereof for injection
  • Cerebroprotein hydrolysate and lyophilized powder thereof for injection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The preparation of embodiment 1 cerebroprotein hydrolyzate

[0038] (1) Take fresh healthy pig brain, remove fat, homogenate, then vacuumize to 8Pa, keep the pressure for 10min, and then inject carbon dioxide to the pressure of 15MPa, keep the pressure for 22min;

[0039] (2) At 400rpm, slowly add the carbon dioxide-saturated fat-free brain pulp into the liquid carbon dioxide whose volume is 6.5 times the weight of the fat-free brain pulp. After adding, stir for 40 minutes, cool down to -75°C, keep for 3 hours, and then put it in 8 minutes. The temperature is raised to 45°C at a constant speed to vaporize the carbon dioxide;

[0040] (3) Add 3 times the weight of water for injection to the frozen-thawed brain pulp obtained in step 2, stir evenly, then add papain equivalent to 0.8% of the weight of the frozen-thawed brain pulp, and hydrolyze at 35°C for 3.5 hours to obtain an enzymatic hydrolyzate;

[0041] (4) Centrifuge the enzymatic hydrolyzate at 2500 rpm for 25 minut...

Embodiment 2

[0042] The preparation of embodiment 2 cerebroprotein hydrolyzate

[0043] (1) Take fresh healthy pig brain, remove fat, homogenate, then vacuumize to 5Pa, keep the pressure for 15min, and then inject carbon dioxide to the pressure of 10MPa, keep the pressure for 25min;

[0044] (2) Slowly add the degreased brain pulp saturated with carbon dioxide into the liquid carbon dioxide whose volume is 5 times the weight of the degreased brain pulp at a rotation speed of 300 rpm. After adding, stir for 60 minutes, cool down to -70°C, keep for 2 hours, and then lower the temperature for 5 minutes. Raise the temperature to 50°C to vaporize the carbon dioxide;

[0045] (3) Add 2 times the weight of water for injection to the frozen-thawed brain pulp obtained in step 2, stir evenly, then add papain equivalent to 0.5% of the weight of the frozen-thawed brain pulp, and hydrolyze at 40°C for 3 hours to obtain an enzymatic hydrolyzate;

[0046] (4) Centrifuge the enzymatic hydrolyzate at 2500...

Embodiment 3

[0047] The preparation of embodiment 3 cerebroprotein hydrolyzates

[0048] (1) Take fresh healthy pig brain, remove fat, homogenate, then vacuumize to 10Pa, keep the pressure for 5min, and then inject carbon dioxide to the pressure of 18MPa, keep the pressure for 20min;

[0049] (2) Slowly add the carbon dioxide-saturated defatted brain pulp into the liquid carbon dioxide whose volume is 8 times the weight of the defatted brain pulp at a rotation speed of 500 rpm. After adding, stir for 20 minutes, cool down to -80°C, keep for 4 hours, and then lower the temperature for 10 minutes. Raise the temperature to 40°C to vaporize the carbon dioxide;

[0050] (3) Add 4 times the weight of water for injection to the frozen-thawed brain plasma obtained in step 2, stir evenly, then add pepsin equivalent to 1% of the weight of the frozen-thawed brain plasma, and hydrolyze at 30°C for 4 hours to obtain an enzymatic hydrolyzate;

[0051] (4) Centrifuge the enzymatic hydrolyzate at 2500rpm...

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Abstract

The invention discloses a cerebroprotein hydrolysate and a preparation method thereof. The extract is prepared by adopting the following method: (1) taking fresh and healthy pig brains, removing fat, homogenizing, then performing vacuum-pumping till 5-10Pa, keeping the pressure for 5-15 minutes, then introducing carbon dioxide until the pressure is 10-18MPa, and keeping the pressure for 20-25 minutes; (2) slowly adding the defatted brains with the saturated carbon dioxide into liquid carbon dioxide in the volume which is 5-8 times of the weight of the defatted brains, stirring for 0.5-2 hours after the end of adding, cooling down to minus 70-minus 80 DEG C, keeping for 2-4 hours, and then increasing the temperature to 40-50 DEG C within 5-10 minutes to gasify the carbon dioxide; (3) adding water for injection in the weight of 2-4 times of that of the frozen-thawed brains obtained in the step (2), uniformly stirring, further adding papain or pepsin which is equivalent to 0.5-1% of the weight of the frozen-thawed brains, and performing hydrolysis at the temperature of 30-40 DEG C for 3-4 hours to get an enzymatic hydrolysate; and (4) centrifugating the enzymatic hydrolysate, taking a supernatant liquid, performing activated carbon adsorption, performing decarbonization and filtering.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a cerebroprotein hydrolyzate and its freeze-dried powder injection. Background technique [0002] Cerebroprotein hydrolyzate is a kind of peptide energy neurotrophic drug unique to the brain. It can act on the central nervous system in various ways, regulate and improve the metabolism of neurons, promote the formation of synapses, induce the differentiation of neurons, and further protect nerve cells from various ischemia and neurotoxin damage. Commonly used for the improvement of symptoms of craniocerebral trauma, sequelae of cerebrovascular disease accompanied by memory loss and attention deficit disorder. The prior art discloses a variety of preparation methods and preparations of cerebroprotein hydrolyzate, such as: [0003] Chinese patent ZL01130523.1 discloses a production process of cerebroprotein hydrolyzate injection, specifically: remove the mucous membrane and blood stains on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/01A61K35/30A61K9/19A61P25/28A61P25/00
Inventor 李立忠王勇解晓荣李润宝苏志强姚荷云闫洁胡成伟
Owner SHANXI PUDE PHARMA CO LTD
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