Method for abating antibiotics resistance gene pollution in water

A technology for antibiotic resistance and water quality, applied in the field of water treatment, can solve problems such as antibiotic resistance gene pollution of water bodies, and achieve the effects of easy industrial application, simple equipment, and simplified treatment process

Inactive Publication Date: 2013-06-19
INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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Problems solved by technology

[0005] 1. The present invention will solve the problem of antibiotic resistance gene pollution of water body, and provide a kind of method for removing antibiotic resistance gene in water body

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  • Method for abating antibiotics resistance gene pollution in water

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Embodiment 1

[0015] A method for removing antibiotic resistance genes in water bodies is carried out according to the following steps: 1. Take 10L of water samples from the wastewater of a certain pig farm in Xiamen City, and through PCR amplification, the following nine kinds of antibiotic resistance genes are detected: integrons Gene int 1. Sulfonamide resistance gene sul 1, sul 2, sul A, Tetracycline resistance gene tet A. tet B. tet E. tet M. tet H; first add coagulant polyaluminum chloride 20mg / L to the water sample, and precipitate for 30min to make the turbidity lower than 20NTU; 2. Filter it through a sand filter to further remove the suspended 3. The effluent obtained in step 2 is imported into an ultraviolet water treatment device equipped with an ultraviolet lamp, and the sewage is treated with ultraviolet radiation. The wavelength range of ultraviolet radiation is 256nm, and the action time of ultraviolet radiation is 30min; , carry out PCR amplification to the ...

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Abstract

The invention discloses a method for abating antibiotics resistance gene pollution in water and belongs to the technical field of water treatment. The method disclosed by the invention aims at solving the problem of the water pollution caused by antibiotics resistance gene. The method comprises the following steps of: firstly removing the content of suspended particles in water through coagulating sedimentation of an ultraviolet ray irradiation method to obtain a further ultraviolet projection distance in subsequent processing; then, through the ultraviolet radiation, generating strong oxidizing substances such as hydroxyl radical on one hand and reacting the substances with the chemical substances composing the antibiotics resistance gene for changing the chemical natures thereof so that the genetic information contained in the antibiotics resistance gene can not be sent, destroying the molecular structure of the antibiotics resistance gene through the ultraviolet physical radiation on the other hand so that the molecular structure is broken into free base segments and the integrity of the genetic information contained therein is lost, thereby achieving the aim of abating antibiotics resistance gene pollution.

Description

technical field [0001] The invention belongs to the field of water treatment, in particular to a method for removing antibiotic resistance genes in water bodies. Background technique [0002] In recent years, antibiotics have been widely used in medicine, animal husbandry and aquaculture in my country. The abuse of antibiotics causes the microbial community system in animals to produce antibiotic resistance genes (antibiotic resistance genes, ARGs) to different types of antibiotics. When these microorganisms enter the environment through livestock feces, livestock carcasses, etc. Sex genes are very easy to spread among bacteria in the environment, and it is possible to produce "super bacteria" that can tolerate a variety of antibiotics. At present, antibiotic resistance genes are a new class of environmental pollutants, and their ecological risks may be far greater than the environmental hazards caused by antibiotics themselves. Antibiotic resistance genes have been listed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F9/08
CPCY02W10/37
Inventor 赵峰王鲁刘玉蕾
Owner INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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