Cut-log cultivation method of polyporusrhinocerus cooke

A cultivation method and technology of tiger milk ganoderma, applied in the field of artificial cultivation of edible and medicinal fungi, can solve the problems of small sclerotia, immature technology, low yield, etc. Effect

Inactive Publication Date: 2013-06-26
HANGZHOU YIHE AGRI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention provides a section wood cultivation method of tiger milk ganoderma, and the technical problem to be solved is: aiming at defects such as immature technology, low yie

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The tiger milk Ganoderma lucidum was purchased from Sanming Fungi Research Institute, and the tiger milk Ganoderma lucidum was expanded and cultured on the solid mother seed medium for 15 days to obtain the tiger milk Ganoderma lucidum mother seed, and then inoculated on the original seed medium for 40 days until the mycelium was full After inoculation on section wooden bags for cultivation;

[0028] The formula of the solid stock medium is: yeast powder 10g, peptone 10g, glucose 20g, potassium dihydrogen phosphate 0.3g, magnesium sulfate heptahydrate 0.2g, agar powder 15g, distilled water 1000mL, pH6.0, sterilized at 121°C for 30min. Original seed medium: 80% broad-leaved wood chips, 18% wheat bran, 1% sucrose, 1% gypsum powder, stir well and add water to make the water content of the medium reach 55-60%; the original seed medium uses a 500mL original seed bottle, The filling volume should not exceed 400ml to ensure sufficient air; pick a small piece of the solid mothe...

Embodiment 2

[0041] Tiger milk ganoderma strains were purchased from the Institute of Microbiology, Chinese Academy of Sciences, and tiger milk ganoderma strains were expanded and cultured on liquid medium for 10-15 days to obtain tiger milk ganoderma parent species.

[0042] The stock medium is liquid medium: 10g of yeast powder, 10g of peptone, 20g of glucose, 0.3g of potassium dihydrogen phosphate, 0.2g of magnesium sulfate heptahydrate, 1000mL of distilled water, pH 6.0, and sterilized by damp heat at 121°C for 25min. Use a 250m1 or 500ml triangular flask with a capacity of 100m1 or 200ml, sterilize at 121°C for 30 minutes, cool to room temperature, inoculate tiger milk ganoderma strains, rotate on a rotary shaker, and cultivate for 10-15 days to become tiger milk ganoderma liquid mother species . The original seed medium uses 80% oak wood chips, 18% wheat bran, 1% sucrose, and 1% gypsum powder. After stirring evenly, add water to make the water content of the medium reach 50-55%. The ...

Embodiment 3

[0045] Tiger milk ganoderma strains are obtained from wild tiger milk ganoderma sclerotia in Hainan. Firstly, the collected wild tiger milk ganoderma sclerotia is cleaned, rinsed with tap water for a few minutes, wiped dry with clean gauze, and put into the ultra-clean workbench. Scrub the sclerotium skin with disinfectant alcohol, rinse it several times with sterile water, dry the surface moisture with sterile filter paper, cut the sclerotia of wild tiger milk Ganoderma lucidum with a sterile scalpel, dig out with an inoculation shovel and cut about 0.4cm square tissue, After sterilizing with 0.1% mercuric chloride for 3 minutes, place the sclerotium tissue in the center of the culture medium with tweezers. After 5 days of incubation at 30°C, white fluffy bacteria can be seen growing from the tissue block. Transfer the test tube 1-2 times to obtain purified tiger milk Ganoderma lucidum mycelium.

[0046] Production is arranged in Hainan Province, which can be carried out thro...

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Abstract

The invention discloses a cut-log cultivation method of polyporusrhinocerus cooke. The method comprises manufacturing of a polyporusrhinocerus cooke parent seed, an original seed and a cut-log cultivation seed, land selecting and preparing, cut-log cultivation seed burying and field managing and collecting. The method overcomes the detects in the prior art, wild growing environment of the polyporusrhinocerus cooke is simulated, the polyporusrhinocerus cooke is cultivated by adopting the cut-log cultivation method, the requirements of nutrition of polyporusrhinocerus cooke sclerotium are met, and therefore superior sclerotium is formed.

Description

technical field [0001] The invention relates to an artificial cultivation method of edible and medicinal fungi, belonging to the field of artificial cultivation of edible and medicinal fungi. Background technique [0002] Tiger Milk Ganoderma Lignosus rhinocerus (Cooke) Ryvarden, belonging to Eumycota, Basidiomycotin, Hymenomycetes, Aphyllophorales, Polyporaceae, Tiger Milk Ganoderma Lignosus, also known as Tiger Milk, is a kind of aboriginal people in Malaysia Widely used edible and medicinal fungi have a history of about 400 years. Mainly distributed in Malaysia, Philippines, Sri Lanka, Australia and East Africa, etc., China is distributed in Hainan Province. The sclerotium is its medicinal part, which has the effects of relieving cough and relieving asthma, invigorating the spleen and eliminating dampness. It is often used in the folk to treat asthma and cough, and the curative effect is excellent. Traditional Chinese medicine believes that it is a good medicine for tr...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 吴吉安陈向东钟耀强曾念开
Owner HANGZHOU YIHE AGRI TECH CO LTD
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