Bacillus subtilis microbial air disinfectant for livestock and poultry farm

A Bacillus subtilis, air disinfectant technology, applied in the field of microorganisms, can solve the problems of low content of active substances, no Bacillus subtilis, inconvenient extraction and use, etc., and achieves low nutritional requirements, ideal disinfection effect, and targeted effects.

Active Publication Date: 2013-07-10
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low content of these active substances, it is inconvenient to extract and use. Therefore, there is no report that Bacillus subtilis is used as a microbial air disinfectant alone at present.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The Bacillus subtilis in Example 1 was purchased from the China General Microorganism Culture Collection Center (CGMCC) with a preservation number of 1.1413.

[0028] Example 1

[0029] A kind of Bacillus subtilis livestock and poultry field microbial air disinfectant is prepared by the following method:

[0030] (1) Inoculate Bacillus subtilis in liquid medium for activation culture, shake culture at 35-38°C and 180 rpm for 12-18 hours to obtain activated bacteria;

[0031] The components of the liquid medium are as follows, all in mass percentage: 0.5% yeast extract, 1% peptone, 1% sodium chloride, pH 7.0.

[0032] (2) Inoculate the activated thalline obtained in step (1) into a liquid fermentation medium at 5% by volume, and ferment for 48 hours at 28°C to obtain a microbial fermentation broth; the pH of the microbial fermentation broth is 7.8, the total amount of bacteria reached 2.1 billion / g.

[0033] Described liquid fermentation culture medium component is as...

Embodiment 2

[0037] A kind of Bacillus subtilis livestock and poultry field microbial air disinfectant is prepared by the following method:

[0038] (1) Inoculate Bacillus subtilis in liquid medium for activation culture, shake culture at 35-38°C and 180 rpm for 12-18 hours to obtain activated bacteria;

[0039] The components of the liquid medium are as follows, all in mass percentage: 0.5% yeast extract, 1% peptone, 1% sodium chloride, pH 7.0.

[0040] (2) Inoculate the activated thalline obtained in step (1) into a liquid fermentation medium at 5% by volume, and ferment for 48 hours at 28°C to obtain a microbial fermentation broth; the pH of the microbial fermentation broth is 7.8, the total amount of bacteria reaches 2.3 billion / g.

[0041] Described liquid fermentation culture medium component is as follows, is weight percent:

[0042] Sodium chloride 0.7%, peptone 0.8%, yeast extract 1%, glucose 1.2%, ammonium carbonate 0.5%, water balance, pH natural.

[0043] (3) Spray-dry the m...

Embodiment 3

[0045] Get the microbial air disinfectant prepared in Example 1 and Example 2, mix it with water in a mass ratio of 1:500, and spray it at a temperature of 28 to 32 °C at a spray rate of 5 milliliters per square meter. In the livestock and poultry breeding shed (disinfect before entering the chicken).

[0046] Before the chicks enter the shed, place five plates with different bacteria in different positions (four corners and the middle position) in the shed, cover them after 5 minutes, take them out, and then spray disinfectant on the shed for 8 hours Then put them into different plates in the same way, take them out after 5 minutes, put them in a constant temperature incubator at 37°C for 24 hours, and count the number of bacteria before and after disinfection respectively. Calculate the bacteriostatic rate, the results are as follows: Staphylococcus aureus 51%, Escherichia coli 45%, Salmonella pullorum 63%, Pasteurella multocida 57%.

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Abstract

The invention relates to a bacillus subtilis microbial air disinfectant for a livestock and poultry farm. The bacillus subtilis microbial air disinfectant for the livestock and poultry farm is prepared by the following steps: (1) inoculating bacillus subtilis into a liquid culture medium and performing activation culture until the logarithmic phase to prepare activated bacteria; (2) inoculating the activated bacteria into a liquid fermentation culture medium and performing fermentation culture to prepare microbial fermentation liquid; and (3) spray-drying the microbial fermentation liquid to prepare the microbial disinfectant. The bacillus subtilis microbial air disinfectant serving as spray for the first time is applied to the livestock and poultry farm and achieves antagonistic, inhibiting or killing effect on pathogenic bacteria by utilizing the growth advantage and the metabolite of the bacillus subtilis so as to disinfect and purify the farm environment.

Description

technical field [0001] The invention relates to a microbial air disinfectant for Bacillus subtilis livestock and poultry farms, belonging to the technical field of microorganisms. Background technique [0002] Worldwide, livestock and poultry diseases caused by pathogenic bacteria are one of the main causes of livestock losses. Moreover, most modern large-scale livestock and poultry are raised in closed pens, and the pathogenic bacteria contained in their excrement and secretions cause the air quality of the livestock and poultry houses to decline, and induce frequent outbreaks of livestock and poultry mass diseases. At present, people mainly use chemical disinfectants to prevent and control such diseases, and have achieved remarkable results. However, many chemical disinfectants have the characteristics of high toxicity, high residue, and refractory degradation. With the long-term unreasonable use of these chemical disinfectants, environmental pollution has become increasi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P1/00A61L9/14A61L101/52
Inventor 楚杰刘可春何秋霞郭刚
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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